Follow-up evaluation with ultrasonography of peripheral nerve injuries after an earthquake

Published data on earthquake-associated peripheral nerve injury is very limited. Ultrasonography has been proven to be efficient in the clinic to diagnose peripheral nerve injury. The aim of this study was to assess the role of ultrasound in the evaluation of persistent peripheral nerve injuries 1 year after the Wenchuan earthquake. Thirty-four patients with persistent clinical symptoms and neurologic signs of impaired nerve function were evaluated with sonography prior to surgi- cal repair. Among 34 patients, ultrasonography showed that 48 peripheral nerves were entrapped, and 11 peripheral nerves were disrupted. There was one case of misdiagnosis on ultrasonogra- phy. The concordance rate of ultrasonographic findings with those of surgical findings was 98%. A total of 48 involved nerves underwent neurolysis and the symptoms resolved. Only five nerves had scar tissue entrapment. Preoperative and postoperative clinical and ultrasonographic results were concordant, which verified that ultrasonography is useful for preoperative diagnosis and postoperative evaluation of injured peripheral nerves.

Trimetallic Metal-Organic Framework Nanoframe Superstructures: A Stress-Buffering Architecture Engineering of Anode Material toward Boosted Lithium Storage Performance

Metal-organic frameworks(MOFs)can serve as prevailing anodes for lithium-ion batteries,due to their multiple redox-active sites and prominent structural compatibility.However,the poor electronic conductivity and inferior cyclability hinder their further implementation.Herein,a synthetic methodology for trimetallic Fe-Co-Ni MOFs with nanoframe superstructures architecture(Fe-Co-Ni NFSs)via structural evolution is proposed for versatile anode materials for lithium storage.Ascribed to optimal compositional and structural optimization,the Fe-Co-Ni NFSs achieve exceptional electrochemical performance with superior specific capacity(1030 mAh g^(−1) at 0.1 A g^(−1)),outstanding rate capacity(414 mAh g^(−1) at 2 A g^(−1)),and prolonged cyclability(489 mAh g^(−1) upon 1000 cycles at 1 A g^(−1)).Both experimental and theoretical investigations reveal that the multi-component metal centers could boost electronic conductivity,confer multiple active sites,and energetically favor Li adsorption capability.Additionally,the nanoframe superstructures of Fe-Co-Ni NFSs could facilitate stress-buffering effect on volumetric expansion and prevent electrode pulverization,further improving the lithium storage capability.This work envisions a meticulous protocol for high-performance MOF anode materials for lithium-ion batteries.

地方应用型本科高校智库高质量发展:功能定位、现实困境与路径创新

[目的/意义]地方应用型本科高校智库通过深度参与政企科学民主依法决策,在知识创新与实践应用中实现高质量发展,充分彰显“应用型”和“地方性”特征,助推应用型本科高校高质量发展。[方法/过程]本文通过文献梳理、数据挖掘与分析、案例阐释等方法,首先归纳地方应用型本科高校智库的功能定位,然后通过整体与局部比较分析出地方应用型本科高校智库建设存在的功能定位模糊、智库人才不足和成果水平有限3个方面的现实困境,最后提出了创新发展路径。[结果/结论]地方应用型本科高校智库具有服务政企决策的基本功能、提升学科建设和科研能力的支撑功能以及提高人才培养质量的独特功能,应通过校地政企深度融合、人才培养深入推进、多措并举深化支撑、智库联盟深刻联系等4个大方面13项具体举措创新建设路径,聚焦微中观社会现实问题,推动智库高质量发展。

APPROXIMATION PROBLEMS ON THE SMOOTHNESS CLASSES

This paper investigates the relative Kolmogorov n-widths of 2π-periodic smooth classes in■.We estimate the relative widths of■and its generalized class K_(p)■(P_(r)),where K_(p)H^(ω)(Pr)is defined by a self-conjugate differential operator P_(r)(D)induced by■Also,the modulus of continuity of the r-th derivative,or r-th self-conjugate differential,does not exceed a given modulus of continuityω.Then we obtain the asymptotic results,especially for the case p=∞,1≤q≤∞.

Dexmedetomidine ameliorates diabetic intestinal injury by promoting the polarization of M2 macrophages through the MMP23B pathway

BACKGROUND Diabetes is often associated with gastrointestinal dysfunctions,which can lead to hypoglycemia.Dexmedetomidine(DEX)is a commonly used sedative in perioperative diabetic patients and may affect gastrointestinal function.AIM To investigate whether sedative doses of DEX alleviate diabetes-caused intestinal dysfunction.METHODS Sedation/anesthesia scores and vital signs of streptozotocin(STZ)-induced diabetic mice under DEX sedation were observed.Diabetic mice were divided into saline and DEX groups.After injecting sedatives intraperitoneally,tight junctions(TJs)and apoptotic levels were evaluated 24 hours later to assess the intestinal barrier function.The role of DEX was validated using Villin-MMP23B flox/flox mice with intestinal epithelial deletion.In vitro,high glucose and hyperosmolarity were used to culture Caco-2 monolayer cells with STZ intervention.Immunofluorescence techniques were used to monitor the barrier and mitochondrial functions.RESULTS MMP23B protein levels in the intestinal tissue of STZ-induced diabetic mice were significantly higher than those in the intestinal tissue of control mice,with the DEX group displaying decreased MMP23B levels.Diabetes-mediated TJ disruption,increased intestinal mucosal permeability,and systemic inflammation in wild-type mice might be reversed by DEX.In Caco-2 cells,MMP23B was associated with increased reactive oxygen species accumulation,mitochondrial membrane potential depolarization,and TJ disruption.CONCLUSION DEX reduces MMP23B,which may potentially contribute to STZ-induced intestinal barrier dysfunction,affecting TJ modification through mitochondrial dysfunction.

基于学科专业一体化建设的应用型人才培养模式改革探索——以山东青年政治学院为例

文章基于学科专业一体化建设理念,对应用型人才培养体系建设与模式改革进行实践研究。依据应用型本科高校人才培养定位、经济社会变革要求和行业产业发展需求,通过研究应用型本科高校学科建设、专业建设、课程教学、产业需求的关系,围绕行业产业相关领域,以递进式和平行式一体化融合优化学科专业知识体系组建学院,推动应用型人才培养模式改革与实践。本研究对深化应用型本科高校教育教学改革,培养大学生的应用创新能力有重要意义。

超声辅助下甲状腺术前甲状旁腺正显影定位初步研究

目的:探讨一种有效、精准的甲状旁腺正显影定位技术,实现甲状腺手术中甲状旁腺正显影精准定位,提高甲状旁腺辨认阳性率,有效保护甲状旁腺,提高手术安全性及根治效果,同时评估彩超检测甲状旁腺的效能。方法:1)对四川省肿瘤肿瘤医院头颈外科拟行甲状腺手术的患者行甲状腺患侧正常甲状旁腺的彩超检查,同时记录彩超下甲状旁腺的情况。2)在甲状腺手术患者知情同意情况下术前在彩超引导下局部注射0.1~0.5 mL亚甲蓝染色定位甲状旁腺,术中记录染色情况,术后记录患者是否出现甲状旁腺功能减退症状,如手足麻木、抽搐等,以及血清PTH、血钙情况。结果:1)研究共纳入拟行甲状腺手术患者50例,共检测出甲状旁腺76枚,总甲状旁腺检出率为54.29%(76/140),上甲状旁腺的检出率为40.00%(28/70),下甲状旁腺的检出率为68.57%(48/70);2)检测出的76枚甲状旁腺在彩超上均表现为中等增强回声结节,回声强度高于甲状腺,边界清楚,回声均匀,形态多为椭圆形、类圆形等;3)研究共纳入33例术前甲状旁腺染色定位患者,术前共染色患侧甲状旁腺50枚,术中共37枚显色,总显色率为74.00%(37/50),在显色的37枚甲状旁腺中有10枚甲状旁腺术中冰冻或PTH Test证实为甲状旁腺。在纳入的33例患者中有9例出现暂时性甲状旁腺功能减退症。结论:彩超对于正常甲状旁腺可有效识别,并且彩超引导下甲状旁腺染色定位可在术前精准定位甲状旁腺,帮助甲状腺术中快速、有效识别。

妊娠期贫血对分娩结局的影响探讨

目的评估妊娠期贫血对分娩结局的影响。方法回顾性分析400例孕妇的病历资料,将200例存在妊娠期贫血的孕妇设置为研究组,将200例健康孕妇设置为对照组。统计两组分娩结局,比较产妇不良妊娠结局、胎儿不良结局的发生率。结果研究组不良妊娠结局总发生率为42.00%,显著高于对照组的6.00%,差异有统计学意义(P<0.05)。研究组胎儿不良结局总发生率为40.00%,显著高于对照组的4.00%,差异有统计学意义(P<0.05)。结论妊娠期贫血会对产妇及胎儿的结局产生严重不良影响,需引起临床高度重视。可通过加强产前宣教、重视药物及膳食补充、动态监测红细胞及血红蛋白水平等方式对孕妇进行干预,及时发现异常并进行针对性处理,以达到预防不良妊娠结局发生的目的。

Novel Reaction of Nitroxide with Hantzsch Dihydropyridines

4-oxo-2. 2, 6, 6-tetramethylpiperidinyl-1-oxy radical (TEMPO), a nitroxide, oxidized 4-substituted Hantzsch 1,4-dihydropyridines (DHP) under UV irradiation in CH3CN to give aromatic products in good yields. A possible mechanism for the oxidation was suggested.

Behavioral Differentiation between Anas poecilorhyncha and Domestic Duck

Anas poecilorhyncha is one of improved variety of mallards, which was the protected bird species listed by International Union for Conservation of Nature （1UCN）. Little is known until now about behavioral characteristics of the mallard. The objective of this study was to compare the behavioral differentials between A. poecilorhyncha and domestic duck in order to make a strategy to manage A. poecilorhyncha under the condition of animal welfare. A total of 180 birds were distributed into six groups with 30 birds for each group. They were placed in a room of 3 m ~ 4 m and fed for one year old. Sansui ducks, a kind of native domestic duck （Sansui laying duck） were used for behavioral comparison. Two different rooms in the same building were applied, one room for A. poecilorhyncha and another one for Sansui ducks. All behaviors for A. poecilorhyncha and Sansui ducks were coded using the program The Observer XT 11.5 （Noldus Information Technology, Beijing）. The duration of observation was from 8：00 am to 18：00 pm daily and lasted 5 d. The results showed that there were similar behavioral percentages between A. poecilorhyncha and domestic duck. Percentages of standing activity spent for A. poecilorhyncha and Sansui duck were 34.59% and 30.25%, respectively. Accordingly, the activities, such as wing plugging, preening and head stretching, were more than 5.51%. The other activities, including walking, drinking and tail wagging, occupied less proportions （〈 3%）. While the specific behaviors, like aggression, alerting, wing dithering, clawing, nodding, pendulum clawing and crawling, took less than 1% of percentage. There were large differences between A. poecilorhyncha and domestic duck when comparing eight behavioral peaks. Lag sequential analysis was used to calculate frequency of transition between a pair of activities. Some parameters were very significant, like the frequency value from foraging converted to drinking in Sansui duck was 369-515, but 37-65 in A. poecilorhyncha. The best explaining could be given that Sansui duck was better domesticated than spot-billed duck. This study provides the basic data to study and develop spot-billed duck.

Effect of Nutritional Diet Therapy in Pediatric Diabetic Patients

Objective:To analyze the effect nutritional diet therapy in pediatric diabetic patients.Methods:The study object was 60 cases of children with diabetes,which were divided into groups and treated separately according to the time of admission,and the treatment effects were compared.Results:After treatment,the glycated hemoglobin level,fasting blood glucose level,blood glucose level at 2 hours after meal,serum calcium level,transferrin level,albumin level,prealbumin level,hemoglobin level,the height,weight,and head circumference of the experimental group were better than those of the control group,and there were significant differences between the groups(P<0.05).Conclusion:The application of nutritional diet therapy in the treatment of pediatric diabetic patients can significantly improve blood glucose levels,nutritional indicators and physical indicators of patients.

应用型本科高校学科专业一体化建设的路径选择与创新

本科教育与专科教育的重要区别之一在于学科对人才培养的贡献度和支撑度。区别于传统大学,应用型本科高校学科建设以解决行业产业实际问题为导向,以培养创新应用型、技术技能型人才为目标。应用型本科高校以学科建设基础为专业发展提供实践应用知识体系,以专业发展和课程建设为基础凝练学科研究方向,以促进"科教+产教"双融合为导向推进学科专业一体化建设。教师以应用型科研成果为载体提升解决实际问题的能力,并为以应用性人才培养为导向的专业建设、课程建设和教材建设提供最新的学科知识体系。

Phase 1 clinical trial demonstrated that MUC1 positive metastatic seminal vesicle cancer can be effectively eradicated by modified Anti-MUC1 chimeric antigen receptor transduced T cells

Recent progress in chimeric antigen receptor-modified T-cell(CAR-T cell) technology in cancer therapy is extremely promising, especially in the treatment of patients with B-cell acute lymphoblastic leukemia. In contrast, due to the hostile immunosuppressive microenvironment of a solid tumor, CAR T-cell accessibility and survival continue to pose a considerable challenge, which leads to their limited therapeutic efficacy. In this study, we constructed two anti-MUC1 CAR-T cell lines. One set of CAR-T cells contained SM3 single chain variable fragment(sc Fv) sequence specifically targeting the MUC1 antigen and co-expressing interleukin(IL) 12(named SM3-CAR). The other CAR-T cell line carried the SM3 sc Fv sequence modified to improve its binding to MUC1 antigen(named p SM3-CAR) but did not co-express IL-12. When those two types of CAR-T cells were injected intratumorally into two independent metastatic lesions of the same MUC1+ seminal vesicle cancer patient as part of an interventional treatment strategy, the initial results indicated no side-effects of the MUC1 targeting CAR-T cell approach, and patient serum cytokines responses were positive. Further evaluation showed that p SM3-CAR effectively caused tumor necrosis, providing new options for improved CAR-T therapy in solid tumors.

Reading Time and DNA Sequence Preference of TET3 CXXC Domain Revealed by Single-Molecule Profiling

Recognition of CpG dinucleotide DNA in epigenetic information flow plays a pivotal role for cellular differentiation and development.The TET3 CXXC domain binds to CpG DNA,serving a basic epigenetic information reading mechanism.During the selective recognition of a CpG motif by a CXXC domain from crowded binding sites in a gene sequence,the protein-DNA interactions are beyond CpG dinu-cleotide.However,the selective binding dynamics of CpG within a long DNA context by epigenetic enzymes have been rarely exploit-ed,which is hard for ensemble methods to probe.Here,we used single-molecule magnetic tweezers to quantitatively examine the dynamics of TET3's CXXC domain on a Hoxa9 promoter DNA.Our single-molecule binding profile revealed that CXXC-DNA interactions involve both CpG motifs and their flanking sequences.The residence time of TET3 CXXC differs by about 1000 times in five distin-guished CpG clusters in the context of a CpG island.Moreover,we performed multi-state hidden Markov modeling analysis on the zip-ping/unzipping dynamics of a CpG hairpin,discovering TET3 CXXC's preference on CpG motifs regarding the-2 to+2 flanking bases.Our results shed light on the selective binding dynamics of a CXXC on a gene sequence,facilitating studies on epigenetic information reading mechanisms.

运用蛋白质组学技术探讨针刀抗KOA兔软骨细胞凋亡的作用机制

ObjectiveTo elucidate the mechanism underlying the inhibitory effect of acupotomy on chondrocyte apoptosis in rabbits with knee osteoarthritis (KOA) through screening, analysis, and verification of apoptosis-related differentially expressed proteins via proteomics.MethodsThirty New Zealand white rabbits were randomly divided into the normal, model, and acupotomy groups, with 10 rabbits in each group. The Vidman method was used to prepare KOA models in the model and acupotomy groups. In the acupotomy group, acupotomy was performed at the midpoints of the patellar ligament and medial and lateral collateral ligaments on the left knees once a week for three weeks. Rabbit articular cartilage protein was quantified in each group, and the proteins with differential expression were profiled via proteomics. The apoptosis-related proteins were further screened, and differentially expressed proteins common among the normal, model, and acupotomy groups were collected for cluster analysis. Pathological changes in chondrocytes were observed via HE staining, and chondrocyte apoptosis was observed under an electron microscope. Quantitative real-time PCR (RT-PCR) and western blot were performed to determine the mRNA and protein expression levels, respectively, of the common apoptosis-related proteins in the three groups.ResultsWestern blot and RT-PCR showed that the protein and mRNA expression levels of TXN, CAP1 and FABP3 were lower (all P < 0.05), whereas that of LTF, SCIN and IL-10 were higher (all P < 0.01) in the acupotomy group than that in the model group.ConclusionThe antiapoptotic effect of acupotomy on chondrocytes in KOA rabbits may be achieved by modulating the protein and mRNA expression of differentially expressed apoptosis-related proteins (TXN, CAP1, FABP3, LTS, SCIN, and IL-10).

卵巢癌中新型DC疫苗的研究进展

近年来,上皮性卵巢癌(epithelial ovarian cancer,EOC)的发病率和死亡率迅速上升,严重威胁女性生命安全和健康。然而目前的标准疗法治疗效果欠佳,无法满足患者的迫切需求。免疫疗法最近已在多种实体瘤中显示出临床益处,这也为卵巢癌的治疗提供了新的思路和方法。作为一种重要的免疫治疗形式,树突状细胞(dendritic cells,DC)疫苗已经显示出较强的诱导免疫应答的能力,其临床实验也取得了一定的进展。下一代测序和生物信息工具等技术的进步,有助于研究出更多的新型癌症疫苗,现就近几年研究的新型DC疫苗做一综述。

Targeting growth hormone function: strategies and therapeutic applications

Human growth hormone(GH)is a classical pituitary endocrine hormone that is essential for normal postnatal growth and has pleiotropic effects across multiple physiological systems.GH is also expressed in extrapituitary tissues and has localized autocrine/paracrine effects at these sites.In adults,hypersecretion of GH causes acromegaly,and strategies that block the release of GH or that inhibit GH receptor(GHR)activation are the primary forms of medical therapy for this disease.Overproduction of GH has also been linked to cancer and the microvascular complications that are associated with diabetes.However,studies to investigate the therapeutic potential of GHR antagonism in these diseases have been limited,most likely due to difficulty in accessing therapeutic tools to study the pharmacology of the receptor in vivo.This review will discuss current and emerging strategies for antagonizing GH function and the potential disease indications.