Attention should be paid to the sulfate reduction behavior in a pressure-bearing leachate saturated zone.In this study,within the relative pressure range of 0–0.6 MPa,the ambient temperature with the highest sulfate ...Attention should be paid to the sulfate reduction behavior in a pressure-bearing leachate saturated zone.In this study,within the relative pressure range of 0–0.6 MPa,the ambient temperature with the highest sulfate reduction rate of 50℃ was selected to explore the difference in sulfate reduction behavior in a pressure-bearing leachate saturated zone.The results showed that the sulfate reduction rate might further increase with an increase in pressure;however,owing to the effect of pressure increase,the generated hydrogen sulfide (H_(2)S) could not be released on time,thereby decreasing its highest concentration by approximately 85%,and the duration extended to about two times that of the atmospheric pressure.Microbial community structure and functional gene abundance analyses showed that the community distribution of sulfate-reducing bacteria was significantly affected by pressure conditions,and there was a negative correlation between disulfide reductase B(dsrB) gene abundance and H_(2)S release rate.Other sulfate reduction processes that do not require disulfide reductase A (dsrA) and dsrB genes may be the key pathways affecting the sulfate reduction rate in the pressure-bearing leachate saturated zone.This study improves the understanding of sulfate reduction in landfills as well as provides a theoretical basis for the operation and management of landfills.展开更多
基金supported by the National Natural Science Foundation of China (Nos.41977331,51778579,21876165 and42177203)the Natural Science Foundation of Zhejiang Province (No.LGF20E080004).
文摘Attention should be paid to the sulfate reduction behavior in a pressure-bearing leachate saturated zone.In this study,within the relative pressure range of 0–0.6 MPa,the ambient temperature with the highest sulfate reduction rate of 50℃ was selected to explore the difference in sulfate reduction behavior in a pressure-bearing leachate saturated zone.The results showed that the sulfate reduction rate might further increase with an increase in pressure;however,owing to the effect of pressure increase,the generated hydrogen sulfide (H_(2)S) could not be released on time,thereby decreasing its highest concentration by approximately 85%,and the duration extended to about two times that of the atmospheric pressure.Microbial community structure and functional gene abundance analyses showed that the community distribution of sulfate-reducing bacteria was significantly affected by pressure conditions,and there was a negative correlation between disulfide reductase B(dsrB) gene abundance and H_(2)S release rate.Other sulfate reduction processes that do not require disulfide reductase A (dsrA) and dsrB genes may be the key pathways affecting the sulfate reduction rate in the pressure-bearing leachate saturated zone.This study improves the understanding of sulfate reduction in landfills as well as provides a theoretical basis for the operation and management of landfills.