早发现、早治疗是改善肝癌患者预后和降低病死率的关键。筛选具有高灵敏度和高特异性的分子识别探针对肝癌的早期诊断、预警、转移和疗效评估等具有重要意义。利用体外指数富集配体系统进化技术(systematic evolution of ligands by exp...早发现、早治疗是改善肝癌患者预后和降低病死率的关键。筛选具有高灵敏度和高特异性的分子识别探针对肝癌的早期诊断、预警、转移和疗效评估等具有重要意义。利用体外指数富集配体系统进化技术(systematic evolution of ligands by exponential enrichment,SELEX)筛选获得的核酸适配体,能够高特异性、高亲和力地与多种靶标(包括小分子、蛋白质、细胞、组织、微生物等)结合,为肝癌的特异识别、早期诊断、病理分型提供了有效方法。本研究主要阐述以肝癌细胞系、肝癌血清、临床肝癌组织为靶标的核酸适配体的筛选研究进展。展开更多
c-Myc was one of the first oncogenes to be identified and its overexpression at the RNA and protein levels has subsequently been linked to a wide variety of human cancers[1]. Overexpression of the c-Myc protein or c-M...c-Myc was one of the first oncogenes to be identified and its overexpression at the RNA and protein levels has subsequently been linked to a wide variety of human cancers[1]. Overexpression of the c-Myc protein or c-Myc gene has been shown in 80% of breast cancers, 70% of colon cancers, 90% of gynecological cancers, 50% of hepatocellular carcinomas and a variety of hematological tumors.展开更多
Multiple links between miRNA activity and cancer have been established. Several miRNAs have been describedas oncogenes while others act as tumour suppressors[1]. MiR-449a is a member of miR-34 family which locates onh...Multiple links between miRNA activity and cancer have been established. Several miRNAs have been describedas oncogenes while others act as tumour suppressors[1]. MiR-449a is a member of miR-34 family which locates onhuman chromosome 5q11.2, a region identified as a susceptibility locus in a variety of malignancies, includingprostate cancer[2]. In line with the tumor-suppressive role of miR-34, miR-449a was shown to be significantly downregulatedin prostate cancer cell lines and tissue relative to normal tissues and plays a critical role in growth ofprostate cancer cells [3;4].展开更多
p53 mainly exerts its function through transcriptional regulation of its targets. In response to various stresssignals, including the DNA damage response after IR, ATM phosphorylates p53 leads to its dissociation from...p53 mainly exerts its function through transcriptional regulation of its targets. In response to various stresssignals, including the DNA damage response after IR, ATM phosphorylates p53 leads to its dissociation from MDM-2, an inhibitor of p53, and accumulation of p53[1]. The accumulation of p53 leads to the transcriptional activation ofits target genes and initiates various cellular responses. Several studies have demonstrated that miRNA expressionand biogenesis are controlled by p53 in cellular response to IR.展开更多
Prostate cancer is the most commonly diagnosed cancer in worldwide men and radiation therapy is one of the most common definitive options for localized prostate cancer[1].MicroRNAs(miRNAs)are small non-coding RNAs tha...Prostate cancer is the most commonly diagnosed cancer in worldwide men and radiation therapy is one of the most common definitive options for localized prostate cancer[1].MicroRNAs(miRNAs)are small non-coding RNAs that specifically regulate gene expression by directly cleaving the targeted mRNAs or inhibiting translation by interacting with the 3′untranslated regions(UTRs)of the mRNA targets to increase or inhibit their translation[2].Increasing evidence suggests that the expression levels of miRNAs significantly change when treated by X ray irradiation,which indicates that miRNAs may play a critical role in the DNA damage response in tumor radiation response[3].展开更多
文摘早发现、早治疗是改善肝癌患者预后和降低病死率的关键。筛选具有高灵敏度和高特异性的分子识别探针对肝癌的早期诊断、预警、转移和疗效评估等具有重要意义。利用体外指数富集配体系统进化技术(systematic evolution of ligands by exponential enrichment,SELEX)筛选获得的核酸适配体,能够高特异性、高亲和力地与多种靶标(包括小分子、蛋白质、细胞、组织、微生物等)结合,为肝癌的特异识别、早期诊断、病理分型提供了有效方法。本研究主要阐述以肝癌细胞系、肝癌血清、临床肝癌组织为靶标的核酸适配体的筛选研究进展。
基金Key Program of National Natural Science Foundation of China (U1432248), National Natural Science Foundation of China (11305226)
文摘c-Myc was one of the first oncogenes to be identified and its overexpression at the RNA and protein levels has subsequently been linked to a wide variety of human cancers[1]. Overexpression of the c-Myc protein or c-Myc gene has been shown in 80% of breast cancers, 70% of colon cancers, 90% of gynecological cancers, 50% of hepatocellular carcinomas and a variety of hematological tumors.
基金Key Program of National Natural Science Foundation of China (U1432248), National Natural Science Foundationof China (10835011, 11205219), Western Talent Program of Chinese Academy of Sciences(Y260230XB0).
文摘Multiple links between miRNA activity and cancer have been established. Several miRNAs have been describedas oncogenes while others act as tumour suppressors[1]. MiR-449a is a member of miR-34 family which locates onhuman chromosome 5q11.2, a region identified as a susceptibility locus in a variety of malignancies, includingprostate cancer[2]. In line with the tumor-suppressive role of miR-34, miR-449a was shown to be significantly downregulatedin prostate cancer cell lines and tissue relative to normal tissues and plays a critical role in growth ofprostate cancer cells [3;4].
基金Key Program of National Natural Science Foundation of China (U1432248), National Natural Science Foundationof China (11205219, 11305224)
文摘p53 mainly exerts its function through transcriptional regulation of its targets. In response to various stresssignals, including the DNA damage response after IR, ATM phosphorylates p53 leads to its dissociation from MDM-2, an inhibitor of p53, and accumulation of p53[1]. The accumulation of p53 leads to the transcriptional activation ofits target genes and initiates various cellular responses. Several studies have demonstrated that miRNA expressionand biogenesis are controlled by p53 in cellular response to IR.
基金Key Program of National Natural Science Foundation of China(U1432248)National Natural Science Foundation of China(11775280)National Natural Science Foundation of China(11665003)。
文摘Prostate cancer is the most commonly diagnosed cancer in worldwide men and radiation therapy is one of the most common definitive options for localized prostate cancer[1].MicroRNAs(miRNAs)are small non-coding RNAs that specifically regulate gene expression by directly cleaving the targeted mRNAs or inhibiting translation by interacting with the 3′untranslated regions(UTRs)of the mRNA targets to increase or inhibit their translation[2].Increasing evidence suggests that the expression levels of miRNAs significantly change when treated by X ray irradiation,which indicates that miRNAs may play a critical role in the DNA damage response in tumor radiation response[3].
