目的观察促胰液素(secretin,SCT)对去卵巢骨质疏松大鼠骨转换指标和骨密度的影响。方法采用双侧卵巢去除法制备绝经后骨质疏松大鼠模型,将60只SD大鼠随机分为假手术组、模型对照组、雌激素治疗组和促胰液素治疗组,每组各15只。干预3个月...目的观察促胰液素(secretin,SCT)对去卵巢骨质疏松大鼠骨转换指标和骨密度的影响。方法采用双侧卵巢去除法制备绝经后骨质疏松大鼠模型,将60只SD大鼠随机分为假手术组、模型对照组、雌激素治疗组和促胰液素治疗组,每组各15只。干预3个月后,测定腰椎骨密度(bone mineral density,BMD),采取ELISA法测定血清I型胶原N前端肽(procollagen I N-Terminal propeptide,PINP)和I型胶原C末端肽(collagen type I C-terminal cross-linked telopeptide,CTX),另使用STRING10.0蛋白相互作用网络分析工具分析骨质疏松相关差异蛋白。结果与假手术组比较,模型对照组、雌激素治疗组、促胰液素治疗组的PINP含量升高(P<0.05),模型对照组的CTX含量升高(P<0.05),模型对照组的BMD下降(P<0.05),雌激素治疗组和促胰液素治疗组的CTX、BMD含量无显著差异(P>0.05);与模型对照组比较,雌激素治疗组、促胰液素治疗组PINP、CTX含量有所下降,而BMD含量升高(P<0.05);雌激素组与促胰液素组之间PINP、CTX、BMD含量无显著差异(P>0.05)。结论促胰液素能改善去卵巢骨质疏松大鼠的PINP和CTX含量,增加骨密度,抑制骨质丢失,具有较好的抗骨质疏松效果。展开更多
Aimed at the problem that Fourier decomposition method(FDM)is sensitive to noise and existing mode mixing cannot accurately extract gearbox fault features,a gear fault feature extraction method combining compound dict...Aimed at the problem that Fourier decomposition method(FDM)is sensitive to noise and existing mode mixing cannot accurately extract gearbox fault features,a gear fault feature extraction method combining compound dictionary noise reduction and optimized FDM(OFDM)is proposed.Firstly,the characteristics of the gear signals are used to construct a compound dictionary,and the orthogonal matching pursuit algorithm(OMP)is combined to reduce the noise of the vibration signal.Secondly,in order to overcome the mode mixing phenomenon occuring during the decomposition of FDM,a method of frequency band division based on the extremum of the spectrum is proposed to optimize the decomposition quality.Then,the OFDM is used to decompose the signal into several analytic Fourier intrinsic band functions(AFIBFs).Finally,the AFIBF with the largest correlation coefficient is selected for Hilbert envelope spectrum analysis.The fault feature frequencies of the vibration signal can be accurately extracted.The proposed method is validated through analyzing the gearbox fault simulation signal and the real vibration signals collected from an experimental gearbox.展开更多
文摘目的观察促胰液素(secretin,SCT)对去卵巢骨质疏松大鼠骨转换指标和骨密度的影响。方法采用双侧卵巢去除法制备绝经后骨质疏松大鼠模型,将60只SD大鼠随机分为假手术组、模型对照组、雌激素治疗组和促胰液素治疗组,每组各15只。干预3个月后,测定腰椎骨密度(bone mineral density,BMD),采取ELISA法测定血清I型胶原N前端肽(procollagen I N-Terminal propeptide,PINP)和I型胶原C末端肽(collagen type I C-terminal cross-linked telopeptide,CTX),另使用STRING10.0蛋白相互作用网络分析工具分析骨质疏松相关差异蛋白。结果与假手术组比较,模型对照组、雌激素治疗组、促胰液素治疗组的PINP含量升高(P<0.05),模型对照组的CTX含量升高(P<0.05),模型对照组的BMD下降(P<0.05),雌激素治疗组和促胰液素治疗组的CTX、BMD含量无显著差异(P>0.05);与模型对照组比较,雌激素治疗组、促胰液素治疗组PINP、CTX含量有所下降,而BMD含量升高(P<0.05);雌激素组与促胰液素组之间PINP、CTX、BMD含量无显著差异(P>0.05)。结论促胰液素能改善去卵巢骨质疏松大鼠的PINP和CTX含量,增加骨密度,抑制骨质丢失,具有较好的抗骨质疏松效果。
基金The National Natural Science Foundation of China(No.51975117)the Key Research&Development Program of Jiangsu Province(No.BE2019086).
文摘Aimed at the problem that Fourier decomposition method(FDM)is sensitive to noise and existing mode mixing cannot accurately extract gearbox fault features,a gear fault feature extraction method combining compound dictionary noise reduction and optimized FDM(OFDM)is proposed.Firstly,the characteristics of the gear signals are used to construct a compound dictionary,and the orthogonal matching pursuit algorithm(OMP)is combined to reduce the noise of the vibration signal.Secondly,in order to overcome the mode mixing phenomenon occuring during the decomposition of FDM,a method of frequency band division based on the extremum of the spectrum is proposed to optimize the decomposition quality.Then,the OFDM is used to decompose the signal into several analytic Fourier intrinsic band functions(AFIBFs).Finally,the AFIBF with the largest correlation coefficient is selected for Hilbert envelope spectrum analysis.The fault feature frequencies of the vibration signal can be accurately extracted.The proposed method is validated through analyzing the gearbox fault simulation signal and the real vibration signals collected from an experimental gearbox.