DNA repair-and nucleotide metabolism-related genes exhibit differential CHG methylation patterns in natural and synthetic polyploids (Brassica napus L.)

Polyploidization plays a crucial role in the evolution of angiosperm species.Almost all newly formed polyploids encounter genetic or epigenetic instabilities.However,the molecular mechanisms contributing to genomic instability in synthetic polyploids have not been clearly elucidated.Here,we performed a comprehensive transcriptomic and methylomic analysis of natural and synthetic polyploid rapeseeds(Brassica napus).Our results showed that the CHG methylation levels of synthetic rapeseed in different genomic contexts(genes,transposon regions,and repeat regions)were signi fi cantly lower than those of natural rapeseed.The total number and length of CHG-DMRs between natural and synthetic polyploids were much greater than those of CG-DMRs and CHH-DMRs,and the genes overlapping with these CHG-DMRs were signi fi cantly enriched in DNA damage repair and nucleotide metabolism pathways.These results indicated that CHG methylation may be more sensitive than CG and CHH methylation in regulating the stability of the polyploid genome of B.napus.In addition,many genes involved in DNA damage repair,nucleotide metabolism,and cell cycle control were significantly differentially expressed between natural and synthetic rapeseeds.Our results highlight that the genes related to DNA repair and nucleotide metabolism display differential CHG methylation patterns between natural and synthetic polyploids and reveal the potential connection between the genomic instability of polyploid plants with DNA methylation defects and dysregulation of the DNA repair system.In addition,it was found that the maintenance of CHG methylation in B.napus might be partially regulated by MET1.Our study provides novel insights into the establishment and evolution of polyploid plants and offers a potentialidea for improving the genomic stability of newly formed Brassica polyploids.

Thermal buckling and postbuckling of FGM circular plates with in-plane elastic restraints

Based on von Karman＇s plate theory, the axisymmetric thermal buckling and post-buckling of the functionally graded material （FGM） circular plates with in- plane elastic restraints under transversely non-uniform temperature rise are studied. The properties of the FGM media are varied through the thickness based on a simple power law. The governing equations are numerically solved by a shooting method. The results of the critical buckling temperature, post-buckling equilibrium paths, and configurations for the in-plane elastically restrained plates are presented. The effects of the in-plane elastic restraints, material property gradient, and temperature variation on the responses of thermal buckling and post-buckling are examined in detail.

Development and Validation of a Prognostic Risk Score System for COVID-19 Inpatients:A Multi-Center Retrospective Study in China

Coronavirus disease 2019(COVID-19)has become a worldwide pandemic.Hospitalized patients of COVID-19 suffer from a high mortality rate,motivating the development of convenient and practical methods that allow clinicians to promptly identify high-risk patients.Here,we have developed a risk score using clinical data from 1479 inpatients admitted to Tongji Hospital,Wuhan,China(development cohort)and externally validated with data from two other centers:141 inpatients from Jinyintan Hospital,Wuhan,China(validation cohort 1)and 432 inpatients from The Third People’s Hospital of Shenzhen,Shenzhen,China(validation cohort 2).The risk score is based on three biomarkers that are readily available in routine blood samples and can easily be translated into a probability of death.The risk score can predict the mortality of individual patients more than 12 d in advance with more than 90%accuracy across all cohorts.Moreover,the Kaplan-Meier score shows that patients can be clearly differentiated upon admission as low,intermediate,or high risk,with an area under the curve(AUC)score of 0.9551.In summary,a simple risk score has been validated to predict death in patients infected with severe acute respiratory syndrome coronavirus 2(SARS-CoV-2);it has also been validated in independent cohorts.

Clinical analysis of peritoneal mesothelioma

A case of Peritoneal mesothelioma(PM)in the Third Affiliated Hospital of Inner Mongolia Medical University was collected and analyzed on the basis of diagnosis,physical examination and treatment.Misdiagnosis of PM is very common since it is a rare disease.So this paper aims to enhance the doctors’awareness of peritoneal mesothelioma during clinical practice.

双排尼龙阻沙网-草方格联合沙障防风效应的风洞实验

为了解双排尼龙阻沙网-草方格联合沙障的防风效应,进行孔隙度为40%、45%、55%的双排尼龙阻沙网-草方格联合沙障风洞实验,研究在6、10、14 m·s^(-1)等3种风速下的风速加速率等值线、防风效能和风速廓线指标。结果表明:(1)在不同风速条件下,双排尼龙阻沙网-草方格联合沙障防风效应随着来流风速增大而明显降低;(2)40%孔隙度双排尼龙阻沙网-草方格联合沙障防风效应较好,而45%孔隙度时防风效应较差,55%孔隙度防风效应最差;(3)双排尼龙阻沙网-草方格联合沙障在防风效应方面存在累加。因此,建议在实际应用中采用40%孔隙度双排尼龙阻沙网-草方格联合沙障,以获得最佳防风效应。

绿色人力资源管理与绿色组织绩效:理论机制与未来研究方向

绿色发展是高质量发展的核心组成部分,人作为第一资源是推动企业绿色转型最为活跃的因素。因此,绿色人力资源管理对绿色组织绩效的作用成为近期热点研究领域。近年来,尽管两者关系的研究逐步增多,但尚无系统性文献综述聚焦其间机制。这不利于系统、深入地思考和澄清为何,乃至是否绿色人力资源管理对绿色组织绩效至关重要的问题。本文通过系统回顾文献,从行为论、资源与能力、社会心理视角归纳了现有理论机制的三大脉络。总体而言,现有研究要么假定绿色人力资源管理能提升个体的绿色动机、态度或知识能力,进而转化为组织层面的绩效;要么依赖绿色文化、绿色创新、绿色领导等组织层面的中介变量进行“从宏观到宏观”的解释。基于此,本文认为,在新质生产力发展背景下,未来研究应以可续管理原理为核心驱动力,深化对绿色人力资源管理如何驱动特定的集体互动行为,以及由此带来的绿色实践积累、绿色增长临界点的形成,以及组织绿色涌现状态的显现,最终形成宏观结果的过程性、动态性和复杂性的理论认知。文章最后讨论了相关研究对绿色人力资源管理实践的启示。

DeceFL:a principled fully decentralized federated learning framework

Traditional machine learning relies on a centralized data pipeline for model training in various applications;however,data are inherently fragmented.Such a decentralized nature of databases presents the serious challenge for collaboration:sending all decentralized datasets to a central server raises serious privacy concerns.Although there has been a joint effort in tackling such a critical issue by proposing privacy-preserving machine learning frameworks,such as federated learning,most state-of-the-art frameworks are built still in a centralized way,in which a central client is needed for collecting and distributing model information(instead of data itself)from every other client,leading to high communication burden and high vulnerability when there exists a failure at or an attack on the central client.Here we propose a principled decentralized federated learning algorithm(DeceFL),which does not require a central client and relies only on local information transmission between clients and their neighbors,representing a fully decentralized learning framework.It has been further proven that every client reaches the global minimum with zero performance gap and achieves the same convergence rate O(1=T)(where T is the number of iterations in gradient descent)as centralized federated learning when the loss function is smooth and strongly convex.Finally,the proposed algorithm has been applied to a number of applications to illustrate its effectiveness for both convex and nonconvex loss functions,time-invariant and time-varying topologies,as well as IID and Non-IID of datasets,demonstrating its applicability to a wide range of real-world medical and industrial applications.

Pre-incubated with BSA-complexed free fatty acids alters ER stress/autophagic gene expression by carboxylated multi-walled carbon nanotube exposure in THP-1 macrophages

Recently we found that multi-walled carbon nanotube (MWCNT) exposure alters the mRNA levels of endoplasmic reticulum (ER) stress/autophagic genes, but the impact of biological molecules on this response is unclear. Herein, we compared the different actions of carboxylated MWCNTs (c-MWCNTs) pre-incubated with bovine serum albumin (BSA) or BSA-complexed free fatty acid (denoted as FFA) on macrophages derived from THP-1 monocytes (denoted as THP-1 macrophages). C-MWCNTs exhibited increased diameter and hydrodynamic size as well as decreased absolute zeta potential value after pre-incubation with BSA or FFA, which suggested a coating effect. Cytotoxicity or oxidative stress were not significantly induced after exposure to BSA-or FFA-coated c-MWCNTs. BSA-pre-incubated c-MWCNTs significantly enhanced the expression of the ER stress gene, DDIT3 and the autophagic genes, ATG5, BECN1, and PLIN2, but the mRNA levels of these genes was significantly decreased by FFA-pre-incubated c-MWCNTs. FFA-pre-incubated c-MWCNTs induced significantly higher lipid accumulation and interleukin-6 (IL-6) protein level compared with BSA-pre-incubated c-MWCNTs, which suggested that FFA-pre-incubated c-MWCNTs may more effectively induce the formation of macrophage foam cells. Collectively, our data indicated that pre-incubation with FFA may influence c-MWCNT-induced ER stress/autophagic gene expression and foam cell formation in THP-1 macrophages.

Effects of Cu^2＋ Counter Ions on the Actuation Performance of Flexible Ionic Polymer Metal Composite Actuators

The resistance of Ionic Polymer Metal Composite （IPMC） electrodes plays an important role in the actuation performance of IPMC actuators. Owing to crack formation on the surface of platinum electrode, the surface resistance of the electrode increases, which greatly limits its actuating performance. In this paper, we proposed a new method of dynamic self-repair electrodes by ex- changing Cu2＋ into the IPMC basement membrane. IPMC actuators with Cu2＋ were prepared and the actuation performance in the air was subsequently measured. Compared with conventional IPMC actuators containing Li＋ counter ions, those containing Cu2＋ counter ions exhibited 2 times - 3 times larger displacement and 2 times -3 times bigger blocking force. In the morphology observation, we found that many small copper particles scattered in the middle of cracks after several bending cycles, which leads to an obvious decrease in electrode resistance. In the Cyclic Voltammetry （CV） scan measurement, we observed that the oxidation reaction of copper alternates with reduction reaction of copper ions with the change of voltage polarity, which was a dynamic process. Based on these analyses, it is concluded that the presence of Cu2＋ can repair the damaged electrodes and induce lower electrode resistance, thus leading to the performance improvement of actuation.