Aims Changes in light and temperature are among the most common and most profound environmental perturbations. The independent effects of light and temperature on photosynthesis and respiration are well studied in sin...Aims Changes in light and temperature are among the most common and most profound environmental perturbations. The independent effects of light and temperature on photosynthesis and respiration are well studied in single leaves, but are less well studied in whole plants. The short and long term influence of light and temperature on carbon use efficiency is also poorly understood, and is commonly modeled to remain constant over a wide range of conditions. We sought to determine the primary effects of changing light at two growth temperatures on photosynthesis, respiration, and their balance, as defined by carbon use efficiency. Methods We separated respiration into growth and maintenance components using whole-canopy gas-exchange in an elevated CO2 environment in a controlled environment, and supplemented that information with tissue analysis. Important findings Decreases in light level decreased carbon use efficiency through a reduction in the maintenance coefficient, increased the growth coefficient, and reduced partitioning of N in protein. Growth temperature did not significantly affect either maintenance or growth respiration coefficients, suggesting that long-term temperature responses can differ greatly from short-term observations.展开更多
Shade tolerance is a highly desirable trait when breeding new bermudagrass(Cynodon spp.)lines,but current screening methods can take many years to complete.There is a clear need for large-scale turfgrass breeding prog...Shade tolerance is a highly desirable trait when breeding new bermudagrass(Cynodon spp.)lines,but current screening methods can take many years to complete.There is a clear need for large-scale turfgrass breeding programs to reliably,accurately,and quickly predict shade tolerance in their germplasm.The objectives of this research were to:(i)build custom chambers to reproducibly estimate photosynthetic characteristics from turfgrass canopies at different light intensities and(ii)determine which photosynthetic characteristics are indicative of past shade performance.A custom-built chamber was constructed to determine average photosynthetic characteristics for the whole plant by studying the turfgrass canopy at natural leaf angles for light interception.Shade tolerant(11-T-56)and shade sensitive(Tifway)bermudagrass cultivars were used to examine the effectiveness of the chamber within an array of photosynthetic characteristics when the grasses were grown in full-sun and 73%shade environments.Light compensation point,chlorophyll content,quantum yield,dark respiration rate,and maximum quantum yield of photosystem II were evaluated in this trial.Based on the results of this study,the authors recommend that light compensation point or maximum quantum yield be further evaluated as an accurate indicator of shade tolerance when performed in the field on spring days with grasses grown in full sun or 73%shade environments.Lower Rd rates and ambient temperatures on spring days appear to minimize unexplained variance in the data,which would allow researchers to better detect genotypic differences during this season.展开更多
基金Supported by the National Aeronautics and Space Administration Advanced Life Support Programand the National Aeronautics and Space Administration Graduate Student Research Program
文摘Aims Changes in light and temperature are among the most common and most profound environmental perturbations. The independent effects of light and temperature on photosynthesis and respiration are well studied in single leaves, but are less well studied in whole plants. The short and long term influence of light and temperature on carbon use efficiency is also poorly understood, and is commonly modeled to remain constant over a wide range of conditions. We sought to determine the primary effects of changing light at two growth temperatures on photosynthesis, respiration, and their balance, as defined by carbon use efficiency. Methods We separated respiration into growth and maintenance components using whole-canopy gas-exchange in an elevated CO2 environment in a controlled environment, and supplemented that information with tissue analysis. Important findings Decreases in light level decreased carbon use efficiency through a reduction in the maintenance coefficient, increased the growth coefficient, and reduced partitioning of N in protein. Growth temperature did not significantly affect either maintenance or growth respiration coefficients, suggesting that long-term temperature responses can differ greatly from short-term observations.
基金funded in part by the University of Georgia College of Agriculture&Environmental Sciences,and by the National Institute of Food and Agriculture,U.S.Department of Agriculture,under award number 2010-51181-21064.
文摘Shade tolerance is a highly desirable trait when breeding new bermudagrass(Cynodon spp.)lines,but current screening methods can take many years to complete.There is a clear need for large-scale turfgrass breeding programs to reliably,accurately,and quickly predict shade tolerance in their germplasm.The objectives of this research were to:(i)build custom chambers to reproducibly estimate photosynthetic characteristics from turfgrass canopies at different light intensities and(ii)determine which photosynthetic characteristics are indicative of past shade performance.A custom-built chamber was constructed to determine average photosynthetic characteristics for the whole plant by studying the turfgrass canopy at natural leaf angles for light interception.Shade tolerant(11-T-56)and shade sensitive(Tifway)bermudagrass cultivars were used to examine the effectiveness of the chamber within an array of photosynthetic characteristics when the grasses were grown in full-sun and 73%shade environments.Light compensation point,chlorophyll content,quantum yield,dark respiration rate,and maximum quantum yield of photosystem II were evaluated in this trial.Based on the results of this study,the authors recommend that light compensation point or maximum quantum yield be further evaluated as an accurate indicator of shade tolerance when performed in the field on spring days with grasses grown in full sun or 73%shade environments.Lower Rd rates and ambient temperatures on spring days appear to minimize unexplained variance in the data,which would allow researchers to better detect genotypic differences during this season.
