This study aimed to show that the physicochemical proprieties obtained by Fourier transform infrared spectroscopy(FTIR), thermogravimetry(TG), and scanning electronic microscopy(SEM) can be useful tools for evaluating...This study aimed to show that the physicochemical proprieties obtained by Fourier transform infrared spectroscopy(FTIR), thermogravimetry(TG), and scanning electronic microscopy(SEM) can be useful tools for evaluating the quality of active pharmaceutical ingredients(APIs) and pharmaceutical products.In addition, a simple, sensitive, and efficient method employing HPLC-DAD was developed for simultaneous determination of lidocaine(LID), ciprofloxacin(CFX) and enrofloxacin(EFX) in raw materials and in veterinary pharmaceutical formulations. Compounds were separated using a Gemini C18(250 mm ?4.6 mm, 5 mm) Phenomenexscolumn, at a temperature of 25 °C, with a mobile phase containing 10 m M of phosphoric acid(p H 3.29): acetonitrile(85.7:14.3, v/v) and a flow rate of 1.5 m L/min. Physicochemical characterization by TG, FTIR, and SEM of raw materials of LID, CFX, and EFX provided information useful for the evaluation, differentiation, and qualification of raw materials. Finally, the HPLC method was proved to be useful for evaluation of raw material and finished products, besides satisfying the need for an analytical method that allows simultaneous determination of EFX, CFX, and LID, which can also be extended to other matrices and applications.展开更多
基金the Brazilian agencies CNPq(Conselho Nacional de Desenvolvimento Científico e Tecnológico)CAPES(Coordenacao de Aperfeicoamento de Pessoal de Nível Superior)+3 种基金FAPES(Fundacao de AmparoàPesquisa e Inovacao do Espírito Santo)FAPEMIG(Fundacao de AmparoàPesquisa do Estado de Minas Gerais)for financial supporta collaborative research project with members of Rede Mineira de Química(RQ-MG)supported by FAPEMIG(Project:REDE-113/10Project:CEX-RED-0010–14)
文摘This study aimed to show that the physicochemical proprieties obtained by Fourier transform infrared spectroscopy(FTIR), thermogravimetry(TG), and scanning electronic microscopy(SEM) can be useful tools for evaluating the quality of active pharmaceutical ingredients(APIs) and pharmaceutical products.In addition, a simple, sensitive, and efficient method employing HPLC-DAD was developed for simultaneous determination of lidocaine(LID), ciprofloxacin(CFX) and enrofloxacin(EFX) in raw materials and in veterinary pharmaceutical formulations. Compounds were separated using a Gemini C18(250 mm ?4.6 mm, 5 mm) Phenomenexscolumn, at a temperature of 25 °C, with a mobile phase containing 10 m M of phosphoric acid(p H 3.29): acetonitrile(85.7:14.3, v/v) and a flow rate of 1.5 m L/min. Physicochemical characterization by TG, FTIR, and SEM of raw materials of LID, CFX, and EFX provided information useful for the evaluation, differentiation, and qualification of raw materials. Finally, the HPLC method was proved to be useful for evaluation of raw material and finished products, besides satisfying the need for an analytical method that allows simultaneous determination of EFX, CFX, and LID, which can also be extended to other matrices and applications.
