In recent years, the rate of infertility has not stopped increasing in the world. The aim of the present study was to evaluate the effects of the aqueous extract of Schumanniophyton magnificum (Rubiaceae) on cyclicity...In recent years, the rate of infertility has not stopped increasing in the world. The aim of the present study was to evaluate the effects of the aqueous extract of Schumanniophyton magnificum (Rubiaceae) on cyclicity, ovulation and gestation in mature rats. Methods: After a qualitative phytochemical analysis of these aqueous extracts, the experimental studies carried out were based on the evaluation of the pro-fertility effects of this extract in mature rats. For this purpose, 35 rats were used for the estrous cyclicity test and treated for 21 days at the end of which vaginal smears were taken and the duration, as well as the frequency of the appearance of the phases of the cycle, were evaluated. The ovulation test was performed on 80 female rats, which were divided into two groups of 40 animals and treated respectively in the morning and evening with distilled water, β-oestradiol or plant extract at doses of 200, 400 and 800 mg/kg. At the end of estrus, the rats were sacrificed, the ovaries were removed and weighed, the hemorrhagic points counted and the blood samples were taken for hormonal studies. The last phase of the study consisted in evaluating the effects of these plant extracts on the evolution of gestation. Thus, 42 mature rats were treated during the periods from the 1st to the 10th day (1st stage), and from the 11th to the 17th day (2nd stage). At the end of these two phases, a laparotomy was performed and the number of implantation sites and corpus luteum was counted. And finally, at parturition, from the 18th to the 22nd day (3rd stage), the number of living pups was performed and the gestational parameters were calculated. Results: Administration at the beginning of prœstrus allowed a significant increase (p S. magnificum extract. On the other hand, a significant decrease in progesterone levels (p S. magnificum at doses of 200, 800 mg/kg (p S. magnificum when compared to the control (23 ± 0.16 d). Conclusion: It appears from all these investigations that the aqueous extract of S. magnificum promotes fertility in the rat but represents a danger for the good development of gestation. All these results obtained would be closely related to the presence of certain chemical compounds contained in these various extracts;which would justify their use in traditional medicine for the treatment of certain cases of female infertility in Cameroon.展开更多
Aqueous roots extract of Alchornea cordifolia was studied to evaluate its traditional antifertility claim. Antifertility effect of Alchornea cordifolia was evaluated according to the stages of pregnancy of female rats...Aqueous roots extract of Alchornea cordifolia was studied to evaluate its traditional antifertility claim. Antifertility effect of Alchornea cordifolia was evaluated according to the stages of pregnancy of female rats. In the first stage, forty female rats were randomly divided into four groups (10 rats/group) and respectively treated with 1 ml distilled water (control), 100, 200 and 400 mg/kg body weight (BW) per day of Alchornea cordifolia. After laparotomy and at parturition, sites of implantation and resorption on the uterine horns were determined. The ovaries and uterus were collected and weighed. Blood samples were collected for hormonal assay. In the second and third stage of pregnancy, treatment schedule and antifertility parameters were assessed as above for the first stage. Treated groups received daily 400 mg/kg bw of extract of Alchornea cordifolia while Control group received distilled water. It was observed in the first stage of pregnancy that, the number of resorption sites significantly (p < 0.05) decreased at 100 and 200 mg/kg. No implantation site was observed at dose 400 mg/kg after laparotomy as well as the number of pups at parturition at all doses. The body weight of ovaries and uterine horns at dose 400 mg/kg significantly (p < 0.05) decreased when compared to the control. The extract dose dependently decreased significantly the concentrations of serum progesterone. In the second stage of pregnancy after laparotomy and at parturition, the number of live fetus and the survival ratio significantly (p < 0.001) decreased and were associated with the vaginal discharge and bleeding of animals. In the third stage of pregnancy, the extract caused an early parturition associated with a decreased number of live fetus and the survival ratio with p The results indicate that aqueous roots extract of Alchornea cordifolia could induce inhibitory effects on reproductive functions in female albino rats.展开更多
文摘In recent years, the rate of infertility has not stopped increasing in the world. The aim of the present study was to evaluate the effects of the aqueous extract of Schumanniophyton magnificum (Rubiaceae) on cyclicity, ovulation and gestation in mature rats. Methods: After a qualitative phytochemical analysis of these aqueous extracts, the experimental studies carried out were based on the evaluation of the pro-fertility effects of this extract in mature rats. For this purpose, 35 rats were used for the estrous cyclicity test and treated for 21 days at the end of which vaginal smears were taken and the duration, as well as the frequency of the appearance of the phases of the cycle, were evaluated. The ovulation test was performed on 80 female rats, which were divided into two groups of 40 animals and treated respectively in the morning and evening with distilled water, β-oestradiol or plant extract at doses of 200, 400 and 800 mg/kg. At the end of estrus, the rats were sacrificed, the ovaries were removed and weighed, the hemorrhagic points counted and the blood samples were taken for hormonal studies. The last phase of the study consisted in evaluating the effects of these plant extracts on the evolution of gestation. Thus, 42 mature rats were treated during the periods from the 1st to the 10th day (1st stage), and from the 11th to the 17th day (2nd stage). At the end of these two phases, a laparotomy was performed and the number of implantation sites and corpus luteum was counted. And finally, at parturition, from the 18th to the 22nd day (3rd stage), the number of living pups was performed and the gestational parameters were calculated. Results: Administration at the beginning of prœstrus allowed a significant increase (p S. magnificum extract. On the other hand, a significant decrease in progesterone levels (p S. magnificum at doses of 200, 800 mg/kg (p S. magnificum when compared to the control (23 ± 0.16 d). Conclusion: It appears from all these investigations that the aqueous extract of S. magnificum promotes fertility in the rat but represents a danger for the good development of gestation. All these results obtained would be closely related to the presence of certain chemical compounds contained in these various extracts;which would justify their use in traditional medicine for the treatment of certain cases of female infertility in Cameroon.
文摘Aqueous roots extract of Alchornea cordifolia was studied to evaluate its traditional antifertility claim. Antifertility effect of Alchornea cordifolia was evaluated according to the stages of pregnancy of female rats. In the first stage, forty female rats were randomly divided into four groups (10 rats/group) and respectively treated with 1 ml distilled water (control), 100, 200 and 400 mg/kg body weight (BW) per day of Alchornea cordifolia. After laparotomy and at parturition, sites of implantation and resorption on the uterine horns were determined. The ovaries and uterus were collected and weighed. Blood samples were collected for hormonal assay. In the second and third stage of pregnancy, treatment schedule and antifertility parameters were assessed as above for the first stage. Treated groups received daily 400 mg/kg bw of extract of Alchornea cordifolia while Control group received distilled water. It was observed in the first stage of pregnancy that, the number of resorption sites significantly (p < 0.05) decreased at 100 and 200 mg/kg. No implantation site was observed at dose 400 mg/kg after laparotomy as well as the number of pups at parturition at all doses. The body weight of ovaries and uterine horns at dose 400 mg/kg significantly (p < 0.05) decreased when compared to the control. The extract dose dependently decreased significantly the concentrations of serum progesterone. In the second stage of pregnancy after laparotomy and at parturition, the number of live fetus and the survival ratio significantly (p < 0.001) decreased and were associated with the vaginal discharge and bleeding of animals. In the third stage of pregnancy, the extract caused an early parturition associated with a decreased number of live fetus and the survival ratio with p The results indicate that aqueous roots extract of Alchornea cordifolia could induce inhibitory effects on reproductive functions in female albino rats.
