Apple replant disease(ARD)is a major limitation to the establishment of economically viable orchards on replant sites due to the buildup and long-term survival of pathogen inoculum.Several soilborne necrotrophic fungi...Apple replant disease(ARD)is a major limitation to the establishment of economically viable orchards on replant sites due to the buildup and long-term survival of pathogen inoculum.Several soilborne necrotrophic fungi and oomycetes are primarily responsible for ARD,and symptoms range from serious inhibition of growth to the death of young trees.Chemical fumigation has been the primary method used for control of ARD,and manipulating soil microbial ecology to reduce pathogen density and aggressiveness is being investigated.To date,innate resistance of apple rootstocks as a means to control this disease has not been carefully explored,partly due to the complex etiology and the difficulty in phenotyping the disease resistance.Molecular defense responses of plant roots to soilborne necrotrophic pathogens are largely elusive,although considerable progress has been achieved using foliar disease systems.Plant defense responses to necrotrophic pathogens consist of several interacting modules and operate as a network.Upon pathogen detection by plants,cellular signals such as the oscillation of Ca^(2+)concentration,reactive oxygen species(ROS)burst and protein kinase activity,lead to plant hormone biosynthesis and signaling.Jasmonic acid(JA)and ethylene(ET)are known to be fundamental to the induction and regulation of defense mechanisms toward invading necrotrophic pathogens.Complicated hormone crosstalk modulates the fine-tuning of transcriptional reprogramming and metabolic redirection,resulting in production of antimicrobial metabolites,enzyme inhibitors and cell wall refortification to restrict further pathogenesis.Transcriptome profiling of apple roots in response to inoculation with Pythium ultimum demonstrated that there is a high degree of conservation regarding the molecular framework of defense responses compared with those observed with foliar tissues.It is conceivable that the timing and intensity of genotype-specific defense responses may lead to different outcomes between rootstocks in response to invasion by necrotrophic pathogens.Elucidation of host defense mechanisms is critical in developing molecular tools for genomics-assisted breeding of resistant apple rootstocks.Due to their perennial nature,use of resistant rootstocks as a component for disease management might offer a durable and cost-effective benefit to tree performance than the standard practice of soil fumigation for control of ARD.展开更多
Apple replant disease(ARD)is a significant economic restraint to the successful re-establishment of new apple orchards on sites previously planted to the same crop.Pythium ultimum,an oomycete,is a significant componen...Apple replant disease(ARD)is a significant economic restraint to the successful re-establishment of new apple orchards on sites previously planted to the same crop.Pythium ultimum,an oomycete,is a significant component of the ARD pathogen complex.Although ethylene(ET)-and jasmonic acid(JA)-mediated defense responses are intensively studied in the foliar pathosystem,the transferability of this knowledge to the interaction between a perennial root system and soilborne pathogens is unknown.The aim of this study was to test the hypothesis that the ET/JA-mediated defense response is conserved in roots of tree crops in response to infection by P.ultimum.Apple genes with the annotated function of ET/JA biosynthesis,MdERF(ethylene response factor)for signaling transduction and a gene encoding a pathogenesis-related(PR)protein(b-chitinase,the target of ERF)were identified from the apple genome sequences.The transcriptional profiles of these genes during P.ultimum infection and after exogenous ET and/or JA treatment were characterized using qRT-PCR.Several genes showed a 10-to 60-fold upregulation in apple root tissue 24-48 h post inoculation(hpi).Exogenous ET and JA treatment exhibited either a positive or negative influence on expression of ET or JA biosynthesis genes,depending upon gene isoforms and the tissue types,while the expression of MdERF and the PR protein encoding gene was upregulated by both ET and JA treatment.Our data are consistent with the hypothesis that ET/JA-mediated defense pathways are functional in the root system of perennial tree species defending soilborne pathogens.展开更多
文摘Apple replant disease(ARD)is a major limitation to the establishment of economically viable orchards on replant sites due to the buildup and long-term survival of pathogen inoculum.Several soilborne necrotrophic fungi and oomycetes are primarily responsible for ARD,and symptoms range from serious inhibition of growth to the death of young trees.Chemical fumigation has been the primary method used for control of ARD,and manipulating soil microbial ecology to reduce pathogen density and aggressiveness is being investigated.To date,innate resistance of apple rootstocks as a means to control this disease has not been carefully explored,partly due to the complex etiology and the difficulty in phenotyping the disease resistance.Molecular defense responses of plant roots to soilborne necrotrophic pathogens are largely elusive,although considerable progress has been achieved using foliar disease systems.Plant defense responses to necrotrophic pathogens consist of several interacting modules and operate as a network.Upon pathogen detection by plants,cellular signals such as the oscillation of Ca^(2+)concentration,reactive oxygen species(ROS)burst and protein kinase activity,lead to plant hormone biosynthesis and signaling.Jasmonic acid(JA)and ethylene(ET)are known to be fundamental to the induction and regulation of defense mechanisms toward invading necrotrophic pathogens.Complicated hormone crosstalk modulates the fine-tuning of transcriptional reprogramming and metabolic redirection,resulting in production of antimicrobial metabolites,enzyme inhibitors and cell wall refortification to restrict further pathogenesis.Transcriptome profiling of apple roots in response to inoculation with Pythium ultimum demonstrated that there is a high degree of conservation regarding the molecular framework of defense responses compared with those observed with foliar tissues.It is conceivable that the timing and intensity of genotype-specific defense responses may lead to different outcomes between rootstocks in response to invasion by necrotrophic pathogens.Elucidation of host defense mechanisms is critical in developing molecular tools for genomics-assisted breeding of resistant apple rootstocks.Due to their perennial nature,use of resistant rootstocks as a component for disease management might offer a durable and cost-effective benefit to tree performance than the standard practice of soil fumigation for control of ARD.
文摘Apple replant disease(ARD)is a significant economic restraint to the successful re-establishment of new apple orchards on sites previously planted to the same crop.Pythium ultimum,an oomycete,is a significant component of the ARD pathogen complex.Although ethylene(ET)-and jasmonic acid(JA)-mediated defense responses are intensively studied in the foliar pathosystem,the transferability of this knowledge to the interaction between a perennial root system and soilborne pathogens is unknown.The aim of this study was to test the hypothesis that the ET/JA-mediated defense response is conserved in roots of tree crops in response to infection by P.ultimum.Apple genes with the annotated function of ET/JA biosynthesis,MdERF(ethylene response factor)for signaling transduction and a gene encoding a pathogenesis-related(PR)protein(b-chitinase,the target of ERF)were identified from the apple genome sequences.The transcriptional profiles of these genes during P.ultimum infection and after exogenous ET and/or JA treatment were characterized using qRT-PCR.Several genes showed a 10-to 60-fold upregulation in apple root tissue 24-48 h post inoculation(hpi).Exogenous ET and JA treatment exhibited either a positive or negative influence on expression of ET or JA biosynthesis genes,depending upon gene isoforms and the tissue types,while the expression of MdERF and the PR protein encoding gene was upregulated by both ET and JA treatment.Our data are consistent with the hypothesis that ET/JA-mediated defense pathways are functional in the root system of perennial tree species defending soilborne pathogens.
