Adaptive optics is becoming a valuable tool for high resolution microscopy,providing correction for aberrations introduced by the refractive index structure of specimens.This is proving particularly promising for appl...Adaptive optics is becoming a valuable tool for high resolution microscopy,providing correction for aberrations introduced by the refractive index structure of specimens.This is proving particularly promising for applications that require images from deep within biological tissue specimens.We review recent developments in adaptive microscopy,including methods and applications.A range of advances in different microscope modalities is covered and prospects for the future are discussed.展开更多
The spectral dispersion of ultrashort pulses allows the simultaneous focusing of light in both space and time,which creates socalled spatiotemporal foci.Such space–time coupling may be combined with the existing holo...The spectral dispersion of ultrashort pulses allows the simultaneous focusing of light in both space and time,which creates socalled spatiotemporal foci.Such space–time coupling may be combined with the existing holographic techniques to give a further dimension of control when generating focal light fields.In the present study,it is shown that a phase-only hologram placed in the pupil plane of an objective and illuminated by a spatially chirped ultrashort pulse can be used to generate threedimensional arrays of spatio-temporally focused spots.By exploiting the pulse front tilt generated at focus when applying simultaneous spatial and temporal focusing(SSTF),it is possible to overlap neighboring foci in time to create a smooth intensity distribution.The resulting light field displays a high level of axial confinement,with experimental demonstrations given through two-photon microscopy and the non-linear laser fabrication of glass.展开更多
基金This work was supported by the following grants:EPSRC(EP/E055818/1)BBSRC(BB/J020907/1)the Wellcome Trust(095927/A/11/Z)
文摘Adaptive optics is becoming a valuable tool for high resolution microscopy,providing correction for aberrations introduced by the refractive index structure of specimens.This is proving particularly promising for applications that require images from deep within biological tissue specimens.We review recent developments in adaptive microscopy,including methods and applications.A range of advances in different microscope modalities is covered and prospects for the future are discussed.
基金supported through funding from the Erlangen Graduate School in Advanced Optical Technologies(SAOT)by the German Research Foundation(DFG)in the framework of the German excellence initiative,the Leverhulme Trust(RPG-2013-044)the European Research Council(ERC)under the Horizon 2020 research and innovation programme(grant agreement no.695140).
文摘The spectral dispersion of ultrashort pulses allows the simultaneous focusing of light in both space and time,which creates socalled spatiotemporal foci.Such space–time coupling may be combined with the existing holographic techniques to give a further dimension of control when generating focal light fields.In the present study,it is shown that a phase-only hologram placed in the pupil plane of an objective and illuminated by a spatially chirped ultrashort pulse can be used to generate threedimensional arrays of spatio-temporally focused spots.By exploiting the pulse front tilt generated at focus when applying simultaneous spatial and temporal focusing(SSTF),it is possible to overlap neighboring foci in time to create a smooth intensity distribution.The resulting light field displays a high level of axial confinement,with experimental demonstrations given through two-photon microscopy and the non-linear laser fabrication of glass.
