This paper reports the fabrication and testing of a helical cell separator that uses insulator-based dielectrophoresis as the driving force of its separation. The helical channel shape’s main advantage is its constan...This paper reports the fabrication and testing of a helical cell separator that uses insulator-based dielectrophoresis as the driving force of its separation. The helical channel shape’s main advantage is its constant curvature radius which generates a constant electric field gradient. The presented separator was fabricated by extruding a sacrificial ink on rotating spindles using a computer-controlled robot. After being assembled, connected to the reservoir and encapsulated in epoxy resin, the ink was removed to create a helical microchannel. The resulting device was tested by circulating polystyrene microbeads of 4 and 10 μm diameter through its channel using a voltage of 900 VDC. The particles were separated with efficiencies of 94.0% and 92.5%, respectively. However, roughness in some parts of the channel and connections that had larger diameters compared to the channel created local electric field gradients which, doubtless, hindered separation. It is a promising device that could lead the way toward portable and affordable medical devices.展开更多
Parkinson’s disease(PD)is a progressive neurodegenerative disorder characterized by motor and non-motor symptoms.More than 200 years after its first clinical description,PD remains a serious affliction that affects a...Parkinson’s disease(PD)is a progressive neurodegenerative disorder characterized by motor and non-motor symptoms.More than 200 years after its first clinical description,PD remains a serious affliction that affects a growing proportion of the population.Prevailing treatments only alleviate symptoms;there is still neither a cure that targets the neurodegenerative processes nor therapies that modify the course of the disease.Over the past decades,several animal models have been developed to study PD.Although no model precisely recapitulates the pathology,they still provide valuable information that contributes to our understanding of the disease and the limitations of our treatment options.This review comprehensively summarizes the different animal models available for Parkinson’s research,with a focus on those induced by drugs,neurotoxins,pesticides,genetic alterations,α-synuclein inoculation,and viral vector injections.We highlight their characteristics and ability to reproduce PD-like phenotypes.It is essential to realize that the strengths and weaknesses of each model and the induction technique at our disposal are determined by the research question being asked.Our review,therefore,seeks to better aid researchers by ensuring a concrete discernment of classical and novel animal models in PD research.展开更多
文摘This paper reports the fabrication and testing of a helical cell separator that uses insulator-based dielectrophoresis as the driving force of its separation. The helical channel shape’s main advantage is its constant curvature radius which generates a constant electric field gradient. The presented separator was fabricated by extruding a sacrificial ink on rotating spindles using a computer-controlled robot. After being assembled, connected to the reservoir and encapsulated in epoxy resin, the ink was removed to create a helical microchannel. The resulting device was tested by circulating polystyrene microbeads of 4 and 10 μm diameter through its channel using a voltage of 900 VDC. The particles were separated with efficiencies of 94.0% and 92.5%, respectively. However, roughness in some parts of the channel and connections that had larger diameters compared to the channel created local electric field gradients which, doubtless, hindered separation. It is a promising device that could lead the way toward portable and affordable medical devices.
基金supported by a grant to ML.from Canadian Institutes of Health Research.ML.also receives a salary award from the Fonds de recherche du Québec in partnership with Parkinson Quebec.
文摘Parkinson’s disease(PD)is a progressive neurodegenerative disorder characterized by motor and non-motor symptoms.More than 200 years after its first clinical description,PD remains a serious affliction that affects a growing proportion of the population.Prevailing treatments only alleviate symptoms;there is still neither a cure that targets the neurodegenerative processes nor therapies that modify the course of the disease.Over the past decades,several animal models have been developed to study PD.Although no model precisely recapitulates the pathology,they still provide valuable information that contributes to our understanding of the disease and the limitations of our treatment options.This review comprehensively summarizes the different animal models available for Parkinson’s research,with a focus on those induced by drugs,neurotoxins,pesticides,genetic alterations,α-synuclein inoculation,and viral vector injections.We highlight their characteristics and ability to reproduce PD-like phenotypes.It is essential to realize that the strengths and weaknesses of each model and the induction technique at our disposal are determined by the research question being asked.Our review,therefore,seeks to better aid researchers by ensuring a concrete discernment of classical and novel animal models in PD research.
