In plants,sugars are the key source of energy and metabolic building blocks.The systemic transport of sugars is essential for plant growth and morphogenesis.Plants evolved intricate molecular networks to effectively d...In plants,sugars are the key source of energy and metabolic building blocks.The systemic transport of sugars is essential for plant growth and morphogenesis.Plants evolved intricate molecular networks to effectively distribute sugars.The dynamic distribution of these osmotically active compounds is a handy tool for regulating cell turgor pressure,an instructive force in developmental biology.In this study,we have investigated the molecular mechanism behind the dual role of the receptor-like kinase CANAR.We functionally characterized a long non-coding RNA,CARMA,as a negative regulator of CANAR.Sugarresponsive CARMA specifically fine-tunes CANAR expression in the phloem,the route of sugar transport.Our genetic,molecular,microscopy,and biophysical data suggest that the CARMA–CANAR module controls the shoot-to-root phloem transport of sugars,allows cells to flexibly adapt to the external osmolality by appropriate water uptake,and thus adjust the size of vascular cell types during organ growth and development.Our study identifies a nexus of plant vascular tissue formation with cell internal pressure monitoring,revealing a novel functional aspect of long non-coding RNAs in developmental biology.展开更多
基金supported by the EMBO long-term fellowship(ALTF 217-2021)by the project JG_2024_003 implemented within the PalackýUniversity Young Researcher Grantsupported by NSF CAREER award 1751385.
文摘In plants,sugars are the key source of energy and metabolic building blocks.The systemic transport of sugars is essential for plant growth and morphogenesis.Plants evolved intricate molecular networks to effectively distribute sugars.The dynamic distribution of these osmotically active compounds is a handy tool for regulating cell turgor pressure,an instructive force in developmental biology.In this study,we have investigated the molecular mechanism behind the dual role of the receptor-like kinase CANAR.We functionally characterized a long non-coding RNA,CARMA,as a negative regulator of CANAR.Sugarresponsive CARMA specifically fine-tunes CANAR expression in the phloem,the route of sugar transport.Our genetic,molecular,microscopy,and biophysical data suggest that the CARMA–CANAR module controls the shoot-to-root phloem transport of sugars,allows cells to flexibly adapt to the external osmolality by appropriate water uptake,and thus adjust the size of vascular cell types during organ growth and development.Our study identifies a nexus of plant vascular tissue formation with cell internal pressure monitoring,revealing a novel functional aspect of long non-coding RNAs in developmental biology.
