期刊文献+
共找到1篇文章
< 1 >
每页显示 20 50 100
Oligonucleotide delivery by chitosan-functionalized porous silicon nanoparticles 被引量:2
1
作者 Morteza Hasanzadeh Kafshgari Bahman Delalat +4 位作者 Wing Yin Tong Frances J. Harding martti kaasalainen Jarno Salonen Nicolas H. Voelcker 《Nano Research》 SCIE EI CAS CSCD 2015年第6期2033-2046,共14页
Porous silicon nanoparficles (pSiNPs) are a promising nanocarrier system for drug delivery owing to their biocompatibility, biodegradability, and non-inflammatory nature. Here, we investigate the fabrication and cha... Porous silicon nanoparficles (pSiNPs) are a promising nanocarrier system for drug delivery owing to their biocompatibility, biodegradability, and non-inflammatory nature. Here, we investigate the fabrication and characterization of thermally hydrocarbonized pSiNPs (THCpSiNPs) and chitosan-coated THCpSiNPs for therapeutic oligonucleotide delivery. Chitosan coating after oligonucleotide loading significantly improves sustained oligonucleotide release and suppresses burst release effects. Moreover, cellular uptake, endocytosis, and cytotoxicity of oligonucleotide-loaded THCpSiNPs have been evaluated in vitro. Standard cell viability assays demonstrate that cells incubated with the NPs at a concentration of 0.1 mg/mL are 95% viable. In addition, chitosan coating significantly enhances the uptake of oligonucleotide-loaded THCpSiNPs across the cell membrane. Moreover, histopathological analysis of liver, kidney, spleen, and skin tissue collected from mice receiving NPs further demonstrates the biocompatible and non-inflammatory properties of the NPs as a gene delivery vehicle for intravenous and subcutaneous administration in vivo. Taken together, these results suggest that THCpSiNPs provide a versatile platform that could be used as efficient vehicles for the intracellular delivery of oligonucleotides for gene therapy. 展开更多
关键词 NANOPARTICLES porous silicon CHITOSAN gene delivery
原文传递
上一页 1 下一页 到第
使用帮助 返回顶部