Most rhizobial inoculants that stimulate legume yield are applied with carriers that enhance root contact. The physicochemical properties of biochar are suitable for microbial growth, and it could be an alternative to...Most rhizobial inoculants that stimulate legume yield are applied with carriers that enhance root contact. The physicochemical properties of biochar are suitable for microbial growth, and it could be an alternative to peat, which comes from decreasing reserves but is the commonest solid inoculant carrier. The aim of the current research was to evaluate biochars as carriers of bradyrhizobia in solid inoculant and as coatings for seeds. Biochars and peat were inoculated with Bradyrhizobium japonicum strain 532C and storage time was assessed. A seed coating system was developed using biochar, bacteria liquid culture, water, and guar gum. The viability of bacteria in the coating and in solid biochar was evaluated at 4°C and 21°C. Two biochars were selected for a germination assay. Finally, greenhouse experimentation investigated the effect of biochar inoculant and seed coating on soybean growth and nutrient uptake. The storage time experiment showed that not all biochars equally sustain bacteria survival over time. The germination assay demonstrated that biochar seed coating had no effect on soybean germination. Greenhouse experimentation indicated that the effect of Pyrovac biochar on soybean growth characteristics and nutrient uptake depended on the fertilizer. The main finding was that biochar solid inoculant positively affected plant growth metrics, root characteristics, and the chemical composition of plants supplied with N-free nutrient solution.展开更多
For a constantly growing human population, healthy and productive soil is critical for sustainable delivery of agricultural products. The soil microorganisms play a crucial role in soil structure and functioning. They...For a constantly growing human population, healthy and productive soil is critical for sustainable delivery of agricultural products. The soil microorganisms play a crucial role in soil structure and functioning. They are responsible for soil formation, ecosystem biogeochemistry, cycling of nutrients and degradation of plant residues and xenobiotics. Certain agricultural treatments, such as fertilizers and pesticides applications, crop rotation, or soil amendment addition, influence the composition, abundance and function of bacteria and fungi in the soil ecosystems. Some of these practices have rather negative effects;others can help soil microorganisms by creating a friendlier habitat or providing nutrients. The changes in microbial community structure cannot be fully captured with traditional methods that are limited only to culturable organisms, which represent less than 1% of the whole population. The use of new molecular techniques such as metagenomics offers the possibility to better understand how agriculture affects soil microbiota. Therefore, the main goal of this review is to discuss how common farming practices influence microbial activity in the soil, with a special focus on pesticides, fertilizers, heavy metals and crop rotation. Furthermore, potential practices to mitigate the negative effects of some treatments are suggested and treatments that can beneficially influence soil microbiota are pointed out. Finally, application of metagenomics technique in agriculture and perspectives of developing efficient molecular tools in order to assess soil condition in the context of microbial activities are underlined.展开更多
Although the quality of organic crops has been intensively studied for over three decades, there is still a lack of conclusive data that can prove the superiority of organic crops over conventional ones. There is no d...Although the quality of organic crops has been intensively studied for over three decades, there is still a lack of conclusive data that can prove the superiority of organic crops over conventional ones. There is no doubt that the organic food is safer due to the lack of pesticide residues;however, it is still not entirely sure whether vegetables and fruits produced in this way are better in the context of nutritional values. The study of commonly used vegetables in the European diet was performed to evaluate the difference in concentration of macro nutrients such as Ca, Mg, Na, N, K and P between organic and conventional crops. Additionally, the soil analysis was performed on the material from two growing systems. The results showed that organically cultivated vegetables had generally a higher level of macro nutrients. Also, soil samples showed higher content of these elements in the organically treated soil. From all vegetables, the highest concentrations of macro elements were found in parsley leaves and celery roots.展开更多
文摘Most rhizobial inoculants that stimulate legume yield are applied with carriers that enhance root contact. The physicochemical properties of biochar are suitable for microbial growth, and it could be an alternative to peat, which comes from decreasing reserves but is the commonest solid inoculant carrier. The aim of the current research was to evaluate biochars as carriers of bradyrhizobia in solid inoculant and as coatings for seeds. Biochars and peat were inoculated with Bradyrhizobium japonicum strain 532C and storage time was assessed. A seed coating system was developed using biochar, bacteria liquid culture, water, and guar gum. The viability of bacteria in the coating and in solid biochar was evaluated at 4°C and 21°C. Two biochars were selected for a germination assay. Finally, greenhouse experimentation investigated the effect of biochar inoculant and seed coating on soybean growth and nutrient uptake. The storage time experiment showed that not all biochars equally sustain bacteria survival over time. The germination assay demonstrated that biochar seed coating had no effect on soybean germination. Greenhouse experimentation indicated that the effect of Pyrovac biochar on soybean growth characteristics and nutrient uptake depended on the fertilizer. The main finding was that biochar solid inoculant positively affected plant growth metrics, root characteristics, and the chemical composition of plants supplied with N-free nutrient solution.
文摘For a constantly growing human population, healthy and productive soil is critical for sustainable delivery of agricultural products. The soil microorganisms play a crucial role in soil structure and functioning. They are responsible for soil formation, ecosystem biogeochemistry, cycling of nutrients and degradation of plant residues and xenobiotics. Certain agricultural treatments, such as fertilizers and pesticides applications, crop rotation, or soil amendment addition, influence the composition, abundance and function of bacteria and fungi in the soil ecosystems. Some of these practices have rather negative effects;others can help soil microorganisms by creating a friendlier habitat or providing nutrients. The changes in microbial community structure cannot be fully captured with traditional methods that are limited only to culturable organisms, which represent less than 1% of the whole population. The use of new molecular techniques such as metagenomics offers the possibility to better understand how agriculture affects soil microbiota. Therefore, the main goal of this review is to discuss how common farming practices influence microbial activity in the soil, with a special focus on pesticides, fertilizers, heavy metals and crop rotation. Furthermore, potential practices to mitigate the negative effects of some treatments are suggested and treatments that can beneficially influence soil microbiota are pointed out. Finally, application of metagenomics technique in agriculture and perspectives of developing efficient molecular tools in order to assess soil condition in the context of microbial activities are underlined.
文摘Although the quality of organic crops has been intensively studied for over three decades, there is still a lack of conclusive data that can prove the superiority of organic crops over conventional ones. There is no doubt that the organic food is safer due to the lack of pesticide residues;however, it is still not entirely sure whether vegetables and fruits produced in this way are better in the context of nutritional values. The study of commonly used vegetables in the European diet was performed to evaluate the difference in concentration of macro nutrients such as Ca, Mg, Na, N, K and P between organic and conventional crops. Additionally, the soil analysis was performed on the material from two growing systems. The results showed that organically cultivated vegetables had generally a higher level of macro nutrients. Also, soil samples showed higher content of these elements in the organically treated soil. From all vegetables, the highest concentrations of macro elements were found in parsley leaves and celery roots.