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Microbial communities biostimulated by ethanol during uranium (VI) bioremediation in contaminated sediment as shown by stable isotope probing 被引量:5
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作者 mary beth leigh Wei-Min WU +8 位作者 Erick CARDENAS Ondrej UHLIK Sue CARROLL Terry GENTRY Terence L. MARSH Jizhong ZHOU Philip JARDINE Craig S. CRIDDLE James M. TIEDJE 《Frontiers of Environmental Science & Engineering》 SCIE EI CAS CSCD 2015年第3期453-464,共12页
Stable isotope probing (SIP) was used to identify microbes stimulated by ethanol addition in microcosms containing two sediments collected from the bioremediation test zone at the US Department of Energy Oak Ridge s... Stable isotope probing (SIP) was used to identify microbes stimulated by ethanol addition in microcosms containing two sediments collected from the bioremediation test zone at the US Department of Energy Oak Ridge site, TN, USA. One sample was highly bioreduced with ethanol while another was less reduced. Microcosms with the respective sediments were amended with ^13C labeled ethanol and incubated for 7 days for SIP. Ethanol was rapidly converted to acetate within 24h accompanied with the reduction of nitrate and sulfate. The accumulation of acetate persisted beyond the 7 d period. Aqueous U did not decline in the microcosm with the reduced sediment due to desorption of U but continuously declined in the less reduced sample. Microbial growth and concomitant 13C-DNA production was detected when ethanol was exhausted and abundant acetate had accumulated in both microcosms. This coincided with U(VI) reduction in the less reduced sample. I3C originating from ethanol was ultimately utilized for growth, either directly or indirectly, by the dominant microbial community members within 7 days of incubation. The microbial community was comprised predominantly of known denitrifiers, sulfate-reducing bacteria and iron (Ⅲ) reducing bacteria including Desulfovibrio, Sphingomonas, Ferribacterium, Rhodanobacter, Geothrix, Thiobacillus and others, including the known U(VI)-redueing bacteria Acidovorax, Anaeromyxobacter, Desulfovibrio, Geobac- ter and Desulfosporosinus. The findings suggest that ethanol biostimulates the U(VI)-reducing microbial com- munity by first serving as an electron donor for nitrate, sulfate, iron (IH) and U(VI) reduction, and acetate which then functions as electron donor for U(VI) reduction and carbon source for microbial growth. 展开更多
关键词 Stable isotope probing (S/P) ETHANOL ACETATE uranium reduction SEDIMENT BIOREMEDIATION
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