Objective:To uncover the molecular prevalence of Giardia duodenalis by PCR-restriction fragment length polymorphism(RFLP)in Zabul city,Iran.Methods:Twenty-four stool samples were collected from 215 patients with suspe...Objective:To uncover the molecular prevalence of Giardia duodenalis by PCR-restriction fragment length polymorphism(RFLP)in Zabul city,Iran.Methods:Twenty-four stool samples were collected from 215 patients with suspected giardiasis by microscopic examination.To increase the sensitivity of the PCR,the total genomic DNA from isolates was extracted by applying glass beads and the QIAamp Kit.A one-step PCR-RFLP method,targeting the glutamate dehydrogenase gene,was utilized to differentiate the assemblages A and B among isolates.Results:The PCR fragment was determined from 30 isolates,RFLP assay of 24 isolates showed 24(100)isolates as Genotype B group BIII.Conclusions:The results with the glutamate dehydrogenase gene assay demonstrated that the predominant subtype of Giardia duodenalis in the area is BIII,which showed animals are the main reservoir of the isolates in this area.展开更多
基金Supported by Zabol University of Medical Sciences(Grant No.1393/3478a.)
文摘Objective:To uncover the molecular prevalence of Giardia duodenalis by PCR-restriction fragment length polymorphism(RFLP)in Zabul city,Iran.Methods:Twenty-four stool samples were collected from 215 patients with suspected giardiasis by microscopic examination.To increase the sensitivity of the PCR,the total genomic DNA from isolates was extracted by applying glass beads and the QIAamp Kit.A one-step PCR-RFLP method,targeting the glutamate dehydrogenase gene,was utilized to differentiate the assemblages A and B among isolates.Results:The PCR fragment was determined from 30 isolates,RFLP assay of 24 isolates showed 24(100)isolates as Genotype B group BIII.Conclusions:The results with the glutamate dehydrogenase gene assay demonstrated that the predominant subtype of Giardia duodenalis in the area is BIII,which showed animals are the main reservoir of the isolates in this area.
