Objective:To find a potent antibiotic producer from the sponge-associated bacteria as well as to profile the important substances.Methods:Sponge collection,bacteria isolation,extraction and characterization of potent ...Objective:To find a potent antibiotic producer from the sponge-associated bacteria as well as to profile the important substances.Methods:Sponge collection,bacteria isolation,extraction and characterization of potent active compounds were carried out for this study.Results:Approximately 59 single strains of bacteria were isolated from this sponge.Totally 40 strains showed activity against Escerichia coli,Staphylococcus aureus and Vibrio eltor.The chemical separation of the potent strain Bacterium sp.Lb.10%.2.1.1.b,using n-phase column chromatography revealed 7 active fractions(7,8,9,10,11,14 and 15).The gas chromatography-mass spectrometer analysis of Fraction 7 indicated some phenolic compounds including 4-nonylphenol,methyl 3-(3,5-di-tert-butyl-4-hydroxyphenyl)propionate,acetosyringone,2,4-bis(1-phenylethyl)phenol,1,2-benzenedicarboxylic acid,mono(2-ethylhexyl)ester,tri(2-ethylhexyl)trimellitate and oleamide.Conclusions:Indeed,this is a preliminary information in profiling chemical substances,produced by Bacterium sp.Lb.10%.2.1.1.b.Further purification and structural chemical determination were needed to find a comprehensive result.展开更多
基金Supported by the Riset Unggulan LIPI(Grant No.3403.02.066A).
文摘Objective:To find a potent antibiotic producer from the sponge-associated bacteria as well as to profile the important substances.Methods:Sponge collection,bacteria isolation,extraction and characterization of potent active compounds were carried out for this study.Results:Approximately 59 single strains of bacteria were isolated from this sponge.Totally 40 strains showed activity against Escerichia coli,Staphylococcus aureus and Vibrio eltor.The chemical separation of the potent strain Bacterium sp.Lb.10%.2.1.1.b,using n-phase column chromatography revealed 7 active fractions(7,8,9,10,11,14 and 15).The gas chromatography-mass spectrometer analysis of Fraction 7 indicated some phenolic compounds including 4-nonylphenol,methyl 3-(3,5-di-tert-butyl-4-hydroxyphenyl)propionate,acetosyringone,2,4-bis(1-phenylethyl)phenol,1,2-benzenedicarboxylic acid,mono(2-ethylhexyl)ester,tri(2-ethylhexyl)trimellitate and oleamide.Conclusions:Indeed,this is a preliminary information in profiling chemical substances,produced by Bacterium sp.Lb.10%.2.1.1.b.Further purification and structural chemical determination were needed to find a comprehensive result.
