Trigonella foenum-graecum seeds extract plays a beneficial role on brain antioxidant and oxidative status in alloxan-induced Wistar rats

Context:Trigonella foenum-graecum(TriFG)exhibits increased scavenger enzymatic activities and reduces the production of reactive oxygen species in diabetic rats.Objective:The present study was aimed to investigate the effect of TriFG on lipid peroxidation levels and antioxidant status in brain tissue of rats exposed to alloxan.Materials and Methods:Healthy male rats(180±10 g)were allocated into five groups.Animals in group 1 maintained on normal tap water served as controls and rats in groups 2,3,4,and 5 were treated as experimental groups.Rats in group 2 were intraperitoneally injected with alloxan(120 mg/kg BW)and treated as diabetic rats,whereas rats in groups 3 and 4 were maintained on same experimental regimen as that of rats in groups 1 and 2,respectively,and in addition,they were orally gavaged with herbal extracts of TriFG(0.25 g/kg BW).Diabetic rats treated with glibenclamide in group 5 were used as positive controls.Results and Discussion:Significant(P<0.001)increase in the antioxidant enzymes with a significant(P<0.001)decrease in the lipid peroxidation levels were observed in the brain tissue of diabetic rats treated with TriFG extract as compared to diabetic and glibenclamide-treated rats.No significant changes were observed in pro-and antioxidant levels in brain tissue of rats treated with TriFG extract alone when compared to normal rats.In diabetic rats,brain mitochondrial and cytosolic enzymes like succinate dehydrogenase,glutamate dehydrogenase,and glucose-6-phosphate dehydrogenase activity levels were significantly(P<0.05)decreased with reversely increased was observed in lactate dehydrogenase activity(P<0.05).Conclusions:The findings of the present study suggested that TriFG,through its antioxidant properties,protects brain tissue by mitigating oxidative stress induced by alloxan-exposed rats.TriFG extract significantly increased the antioxidant and oxidative properties in diabetic rats when compared with the control group rats.