Aim:Patients with glioblastomas demonstrate well‑documented immunological impairments including decreased numbers of mature dendritic cells(DCs).Recent data identified karyopherin a2(KPNA2),a nucleocytoplasmic shuttli...Aim:Patients with glioblastomas demonstrate well‑documented immunological impairments including decreased numbers of mature dendritic cells(DCs).Recent data identified karyopherin a2(KPNA2),a nucleocytoplasmic shuttling receptor,as diagnostic and prognostic biomarker for gliomas.The aim of this ongoing study is to correlate parameters of immunity and nucleocytoplasmic transport in glioblastoma patients.Methods:We preoperatively collected serum from 17 patients with glioblastomas and determined DC subsets(HLA DR+Lin-,CD34-,CD45+,CD123+,CD11+were analyzed)using a 6‑color flow cytometry panel.Expression levels of KPNA2 and nuclear accumulation of p53 were evaluated semi‑quantitatively by immunohistochemistry.O6‑methylguanine DNA methyltransferase(MGMT)and isocitrate dehydrogenase‑1(IDH‑1)status were assessed by pyrosequencing and immunohistochemistry,respectively.Results:Median expression levels for both KPNA2 and p53 were 5-10%.IDH‑1‑R132H mutation and MGMT promoter hypermethylation was detected in 3/16 and 1/9 patients,respectively.Mean counts of total mature DCs,myeloid DCs and plasmacytoid DCs were 9.6,2.1,3.4 cells/μL.A preliminary analysis suggests an association between low KPNA2 nuclear expression and increased numbers of mature DCs.However,this correlation did not reach statistical significance so far(P=0.077).Conclusion:Our preliminary data may indicate a role of KPNA2 in the impaired maturation of DCs observed in glioblastoma patients.展开更多
A curved image sensor on plastic foil has been developed for cone beam computed tomography(CBCT)X-ray imaging.The image sensor of about 6×8 cm^(2) size has been built on a thin polyimide foil with an indium galli...A curved image sensor on plastic foil has been developed for cone beam computed tomography(CBCT)X-ray imaging.The image sensor of about 6×8 cm^(2) size has been built on a thin polyimide foil with an indium gallium zinc oxide(IGZO)backplane and an organic photodetectors(OPD)frontplane.A flexible cesium iodide(CsI)scintillator has been attached to the optical sensor with 480×640 pixels of 126µm size.Dark current density of the OPD was low with less than 10^(−7) mA/cm^(2) at−2 V,while an external quantum efficiency(EQE)of about 50%was reached in the visible wavelength range matched to the scintillator output.The image quality of the digital X-ray detector allowed for 3D reconstruction images of a bone phantom on a rotating stage with a lab setup.The curved detector with 32 cm curvature radius opens up the path for very compact CBCT gantries with largely reduced footprint.展开更多
文摘Aim:Patients with glioblastomas demonstrate well‑documented immunological impairments including decreased numbers of mature dendritic cells(DCs).Recent data identified karyopherin a2(KPNA2),a nucleocytoplasmic shuttling receptor,as diagnostic and prognostic biomarker for gliomas.The aim of this ongoing study is to correlate parameters of immunity and nucleocytoplasmic transport in glioblastoma patients.Methods:We preoperatively collected serum from 17 patients with glioblastomas and determined DC subsets(HLA DR+Lin-,CD34-,CD45+,CD123+,CD11+were analyzed)using a 6‑color flow cytometry panel.Expression levels of KPNA2 and nuclear accumulation of p53 were evaluated semi‑quantitatively by immunohistochemistry.O6‑methylguanine DNA methyltransferase(MGMT)and isocitrate dehydrogenase‑1(IDH‑1)status were assessed by pyrosequencing and immunohistochemistry,respectively.Results:Median expression levels for both KPNA2 and p53 were 5-10%.IDH‑1‑R132H mutation and MGMT promoter hypermethylation was detected in 3/16 and 1/9 patients,respectively.Mean counts of total mature DCs,myeloid DCs and plasmacytoid DCs were 9.6,2.1,3.4 cells/μL.A preliminary analysis suggests an association between low KPNA2 nuclear expression and increased numbers of mature DCs.However,this correlation did not reach statistical significance so far(P=0.077).Conclusion:Our preliminary data may indicate a role of KPNA2 in the impaired maturation of DCs observed in glioblastoma patients.
文摘A curved image sensor on plastic foil has been developed for cone beam computed tomography(CBCT)X-ray imaging.The image sensor of about 6×8 cm^(2) size has been built on a thin polyimide foil with an indium gallium zinc oxide(IGZO)backplane and an organic photodetectors(OPD)frontplane.A flexible cesium iodide(CsI)scintillator has been attached to the optical sensor with 480×640 pixels of 126µm size.Dark current density of the OPD was low with less than 10^(−7) mA/cm^(2) at−2 V,while an external quantum efficiency(EQE)of about 50%was reached in the visible wavelength range matched to the scintillator output.The image quality of the digital X-ray detector allowed for 3D reconstruction images of a bone phantom on a rotating stage with a lab setup.The curved detector with 32 cm curvature radius opens up the path for very compact CBCT gantries with largely reduced footprint.
