Genome editing serves as a powerful approach to interrogate the functions of both coding and noncoding sequences.In particular,clustered regularly interspaced short palindromic repeat(CRISPR)/CRISPR associated protein...Genome editing serves as a powerful approach to interrogate the functions of both coding and noncoding sequences.In particular,clustered regularly interspaced short palindromic repeat(CRISPR)/CRISPR associated protein 9(Cas9)system-based editing tools have revolutionized the way we study genome function in mammalian cells,and are being widely used for interrogating critical genes and DNA elements essential for many biological processes.Here,we review CRISPR/Cas9-based genetic tools with an emphasis on CRISPR-mediated high throughput genetic screens in the mammalian genome.展开更多
文摘Genome editing serves as a powerful approach to interrogate the functions of both coding and noncoding sequences.In particular,clustered regularly interspaced short palindromic repeat(CRISPR)/CRISPR associated protein 9(Cas9)system-based editing tools have revolutionized the way we study genome function in mammalian cells,and are being widely used for interrogating critical genes and DNA elements essential for many biological processes.Here,we review CRISPR/Cas9-based genetic tools with an emphasis on CRISPR-mediated high throughput genetic screens in the mammalian genome.
