多端直流系统分区协调保护策略

针对多端直流系统的故障保护问题,首先分析了直流故障特性及多端直流系统保护难点,总结了现有多端直流系统故障保护方法。然后,基于一种典型的多端直流系统拓扑结构,以使用最少数量直流断路器为目标,提出了多端直流系统区域保护划分原则和分区保护策略。该方法将直流故障区域通过直流断路器与非故障区域进行隔离,从而使非故障区域可以继续维持正常运行,而故障区域则通过换流站交流侧断路器和直流侧开关进行保护和隔离。最后,通过MATLAB/Simulink仿真平台建模及仿真分析,验证了多端直流系统分区保护的有效性和经济性。

Genetic mapping of quantitative trait loci for the stigma exsertion rate in rice(Oryza sativa L.)

The efficiency of hybrid rice seed production can be improved by increasing the percentage of exserted stigmas. To identify quantitative trait loci （QTLs） for this trait, we conducted QTL mapping using 75 chromosome segment substitution lines （CSSLs） developed from a cross between the donor parent, Xieqingzao B （XQZB）, a maintainer line which has high stigma exsertion and the recurrent parent, Zhonghui 9308 （ZH9308）, a restorer line which has low stigma exsertion. Atotal of nine QTLs （qSSE5, qSSE10, qSSE11, qDSE10, qDSE11, qTSE5, qTSE6, qTSE10, and qTSE11） for single stigma exsertion （ssE）, dual stigma exsertion （DSE） and total stigma exsertion （TSE） were assessed in two environments （Hainan and Zhejiang）. Six of these QTLs （qSSE10, qSSE11, qDSE10, qDSE11, qTSE10, and qTSE11） were found in both environ- ments, while one QTL （qTSE6） was found in only Hainan, and two QTLs （qSSE5 and qTSE5） were found in only Zhejiang. The qSSE10, qSSE11, qDSE10, qDSE11, qTSE6, qTSE10, and qTSE11 alleles, which are derived from the parent XQZB, exhibited a positive additive effect. In contrast, the qSSE5 and qTSE5 alleles, which are derived from the parent ZH9308, exhibited a negative additive effect. The SSE, DSE and TSE traits were significantly correlated with each other in an en- vironmentally dependent manner. These results indicated that the lines showing higher values for SSE were more likely to exhibit increased values for DSE, which would ultimately increase TSE. To evaluate the advantage of exserted stigmas for cross-pollination, single, dual and total stigma exsertion should be considered separately in future attempts at genetic improvement to achieve increased production of rice hybrid seeds. This study also provides information for fine mapping, gene cloning and particularly marker-assisted selection （MAS）, on the latter and with an emphasis the phenotypic effects and implications of the QTLs for practical use in hybrid rice breeding.

Protection of large partitioned MTDC Networks Using DC-DC converters and circuit breakers

This paper proposes a DC fault protection strategy for large multi-terminal HVDC(MTDC)network where MMC based DC-DC converter is configured at strategic locations to allow the large MTDC network to be operated interconnected but partitioned into islanded DC network zones following faults.Each DC network zone is protected using either AC circuit breakers coordinated with DC switches or slow mechanical type DC circuit breakers to minimize the capital cost.In case of a DC fault event,DC-DC converters which have inherent DC fault isolation capability provide‘firewall’between the faulty and healthy zones such that the faulty DC network zone can be quickly isolated from the remaining of the MTDC network to allow the healthy DC network zones to remain operational.The validity of the proposed protection arrangement is confirmed using MATLAB/SIMULINK simulations.

Demarcating the membrane damage for the extraction of functional mitochondria

Defective mitochondria have been linked to several critical human diseases such as neurodegenerative disorders,cancers and cardiovascular disease.However,the detailed characterization of mitochondria has remained relatively unexplored,largely due to the lack of effective extraction methods that may sufficiently retain the functionality of mitochondria,particularly when limited amount of sample is considered.In this study,we explore the possibility of modulating hydrodynamic stress through a cross-junction geometry at microscale to selectively disrupt the cellular membrane while mitochondrial membrane is secured.The operational conditions are empirically optimized to effectively shred the cell membranes while keeping mitochondria intact for the model mammalian cell lines,namely human embryonic kidney cells,mouse muscle cells and neuroblastoma cells.Unsurprisingly,the disruption of cell membranes with higher elastic moduli(neuroblastoma)requires elevated stress.This study also presents a comparative analysis of total protein yield and concentrations of extracted functional mitochondria with two commercially available mitochondria extraction approaches,the Dounce Homogenizer and the Qproteome®Mitochondria Isolation Kit,in a range of cell concentrations.Our findings show that the proposed“microscale cell shredder”yields at least 40%more functional mitochondria than the two other approaches and is able to preserve the morphological integrity of extracted mitochondria,particularly at low cell concentrations(5–20×10^(4) cells/mL).Characterized by its capability of rapidly processing a limited quantity of samples(200μL),demarcating the membrane damage through the proposed microscale cell shredder represents a novel strategy to extract subcellular organelles from clinical samples.