This study evaluated the therapeutic effects of the phytomedicine,ethyl acetate fraction of the Cynanchum peniculatum (EACP) and bee venom (BV) of the honey bee (Apis mellifera) for the treatment of experimental stifl...This study evaluated the therapeutic effects of the phytomedicine,ethyl acetate fraction of the Cynanchum peniculatum (EACP) and bee venom (BV) of the honey bee (Apis mellifera) for the treatment of experimental stifle osteoarthritis (OA) in 24 skeletally mature mixed small breed dogs (age 2 to 6 years,weight 2. 8 to 9 kg). One year after induction of OA,the animals were randomly allocated in 2 groups; the EACP-group received 10% solution of EACP 0. 5mL plus 0. 2 mL lidocaine; and the BV-group received 10% solution of BV 0. 5 mL plus 0. 2 mL lidocaine intraarticularly twice in a week in the OA-induced stifles for one month. The joint tissue specimens,synovial fluid (SF) and blood samples were collected prior to and 12 months after induction of OA,and one month posttreatment. TRAP levels in SF and serum were measured using a spectrophotometer,and TRAP-positive cells in joint tissues were identified by enzyme histochemistry. TIMP-2 in SF and serum was detected by ELISA. Histochemistry revealed an increased number of TRAP positive cells in tissues from OA-affected joints which was decreased after one month treatment with BV and EACP. After one-month administration of EACP and BV,the levels of TRAP in SF of the index stifles as well as that in the serum were significantly decreased (P<0. 05),whereas,the levels of TIMP-2 in SF of the index stifles as well as that in the serum were increased indicating the therapeutic effects of the EACP and BV on improvement of the conditions of OA.The intraarticular administration of EACP and BV were found effective for the treatment of OA in the dog.展开更多
文摘This study evaluated the therapeutic effects of the phytomedicine,ethyl acetate fraction of the Cynanchum peniculatum (EACP) and bee venom (BV) of the honey bee (Apis mellifera) for the treatment of experimental stifle osteoarthritis (OA) in 24 skeletally mature mixed small breed dogs (age 2 to 6 years,weight 2. 8 to 9 kg). One year after induction of OA,the animals were randomly allocated in 2 groups; the EACP-group received 10% solution of EACP 0. 5mL plus 0. 2 mL lidocaine; and the BV-group received 10% solution of BV 0. 5 mL plus 0. 2 mL lidocaine intraarticularly twice in a week in the OA-induced stifles for one month. The joint tissue specimens,synovial fluid (SF) and blood samples were collected prior to and 12 months after induction of OA,and one month posttreatment. TRAP levels in SF and serum were measured using a spectrophotometer,and TRAP-positive cells in joint tissues were identified by enzyme histochemistry. TIMP-2 in SF and serum was detected by ELISA. Histochemistry revealed an increased number of TRAP positive cells in tissues from OA-affected joints which was decreased after one month treatment with BV and EACP. After one-month administration of EACP and BV,the levels of TRAP in SF of the index stifles as well as that in the serum were significantly decreased (P<0. 05),whereas,the levels of TIMP-2 in SF of the index stifles as well as that in the serum were increased indicating the therapeutic effects of the EACP and BV on improvement of the conditions of OA.The intraarticular administration of EACP and BV were found effective for the treatment of OA in the dog.
