Plants have been used as good bio-indicators and genetic toxicity of environmental pollution in recent years. In this study, aquatic plants Hydrilla verticillata and Ceratophyllum demersum treated with 10umol/L Cd, 5 ...Plants have been used as good bio-indicators and genetic toxicity of environmental pollution in recent years. In this study, aquatic plants Hydrilla verticillata and Ceratophyllum demersum treated with 10umol/L Cd, 5 umol/L Hg, and 20 umol/L Cu for 96 h, showed changes in chlorophyll, protein content, and in DNA profiles. The changes in DNA profiles included variation in band intensity, presence or absence of certain bands and even appearance of new bands. Genomic template stability test performed for the qualitative measurement of changes in randomly amplified polymorphic DNA （RAPD） profiles, showed significant effect at the given concentration of metals. Cloning and sequencing of bands suggested that these markers although may not be homologous to any known gene but its conversion as a sequence characterized amplified region （SCAR） marker is useful in detecting the effects of genotoxin agents.展开更多
Arsenic(As),one of the most harmful toxicant at the global level,severely affects plant metabolism when taken up.Interestingly,the presence of silicon(Si)as a fertilizer in As-contaminated soil is an effective strateg...Arsenic(As),one of the most harmful toxicant at the global level,severely affects plant metabolism when taken up.Interestingly,the presence of silicon(Si)as a fertilizer in As-contaminated soil is an effective strategy to decrease As accumulation in plants.Brassica juncea(var.Varuna)were grown hydroponically to investigate the role of Si at biochemical and molecular levels under arsenite(As^3+)stress.Seedlings of B.juncea were exposed to As^3+,Si,and a combination of both elements.Our data demonstrated that seedlings exposed to As^3+showed an inhibition in shoot length,chlorophyll,carotenoid,and protein,while co-application of Si improved these growth parameters.Silicon supplementation reduced As accumulation in shoot.Increase/decrease was observed in stress-related parameters(cysteine and proline),antioxidant enzymes(superoxide dismutase,ascorbate peroxidase,and catalase),and oxidative stress markers(malondialdehyde and H2O2),which were improved upon co-application of Si as compared to As^3+alone treatment.Random amplified polymorphic DNA(RAPD)is a suitable biomarker assay for plants for assessing the genotoxicity.Seven RAPD primers produced a total of 39 and 48 bands in the leaves of the untreated and treated seedlings,respectively.The RAPD band-profiles and genomic template stability were consistent with other growth and physiological parameters.In conclusion,the genotoxic alterations along with the biochemical parameters indicate that the exposure to Si mitigates As^3+-induced oxidative stress by improving the stress-related parameters and antioxidant system in B.juncea.展开更多
基金supported financially by Department of Science and Technology, New Delhi, India under the SERC-DST fasttrack scheme.
文摘Plants have been used as good bio-indicators and genetic toxicity of environmental pollution in recent years. In this study, aquatic plants Hydrilla verticillata and Ceratophyllum demersum treated with 10umol/L Cd, 5 umol/L Hg, and 20 umol/L Cu for 96 h, showed changes in chlorophyll, protein content, and in DNA profiles. The changes in DNA profiles included variation in band intensity, presence or absence of certain bands and even appearance of new bands. Genomic template stability test performed for the qualitative measurement of changes in randomly amplified polymorphic DNA （RAPD） profiles, showed significant effect at the given concentration of metals. Cloning and sequencing of bands suggested that these markers although may not be homologous to any known gene but its conversion as a sequence characterized amplified region （SCAR） marker is useful in detecting the effects of genotoxin agents.
基金The first author thanks Department of Biotechnology,Government of India for fellowship(No.DBT/JRF/14/AL/250).
文摘Arsenic(As),one of the most harmful toxicant at the global level,severely affects plant metabolism when taken up.Interestingly,the presence of silicon(Si)as a fertilizer in As-contaminated soil is an effective strategy to decrease As accumulation in plants.Brassica juncea(var.Varuna)were grown hydroponically to investigate the role of Si at biochemical and molecular levels under arsenite(As^3+)stress.Seedlings of B.juncea were exposed to As^3+,Si,and a combination of both elements.Our data demonstrated that seedlings exposed to As^3+showed an inhibition in shoot length,chlorophyll,carotenoid,and protein,while co-application of Si improved these growth parameters.Silicon supplementation reduced As accumulation in shoot.Increase/decrease was observed in stress-related parameters(cysteine and proline),antioxidant enzymes(superoxide dismutase,ascorbate peroxidase,and catalase),and oxidative stress markers(malondialdehyde and H2O2),which were improved upon co-application of Si as compared to As^3+alone treatment.Random amplified polymorphic DNA(RAPD)is a suitable biomarker assay for plants for assessing the genotoxicity.Seven RAPD primers produced a total of 39 and 48 bands in the leaves of the untreated and treated seedlings,respectively.The RAPD band-profiles and genomic template stability were consistent with other growth and physiological parameters.In conclusion,the genotoxic alterations along with the biochemical parameters indicate that the exposure to Si mitigates As^3+-induced oxidative stress by improving the stress-related parameters and antioxidant system in B.juncea.
