Inoculation of alfalfa seedlings with root growth promoting microorganisms under semi-arid climate condition may improve biomass production and nutritive value.The current study aimed to investigate the effect of inoc...Inoculation of alfalfa seedlings with root growth promoting microorganisms under semi-arid climate condition may improve biomass production and nutritive value.The current study aimed to investigate the effect of inoculation of alfalfa seedlings with Piriformospora indica(Pi) and co-inoculating Pi with Glomus intraradices(Gi+Pi) or Sinorhizobium meliloti(Sm+Pi) on hay yield,chemical composition,molecular structures by Fourier transformed infrared(FTIR) spectroscopy,in situ ruminal degradability and in vitro gas production.Seedlings were grown in experimental pots in a greenhouse until first cut and then transferred outside and cut a further 4 times.Biomass yield was similar across the treatments.Acid detergent fiber(ADF) concentration was higher in Pi than in control hay,and ADF decreased further with co-inoculation(P < 0.05).The ether extract(EE) concentration was lower for Pi and Gi+Pi compared with control hay,and control,Pi and Gi+Pi hays had lower EE concentration compared with Sm+Pi(P < 0.05).The FTIR spectroscopic vibration peak height ratio related to proteins(amide1+amide 2): total carbohydrate ratio was lower for the inoculation treatments compared with control hay(P < 0.05).In situ ruminal degradability of dry matter and organic matter were higher for hay of inoculated and co-inoculated seedlings than for control hay(P < 0.05).In conclusion,hay of alfalfa seedlings inoculated and co-inoculated with root growth promoting microorganisms had improved nutritional value compared with hay from non-treated alfalfa seedlings,and co-inoculation was the most effective,however,changes were relatively minor.展开更多
文摘Inoculation of alfalfa seedlings with root growth promoting microorganisms under semi-arid climate condition may improve biomass production and nutritive value.The current study aimed to investigate the effect of inoculation of alfalfa seedlings with Piriformospora indica(Pi) and co-inoculating Pi with Glomus intraradices(Gi+Pi) or Sinorhizobium meliloti(Sm+Pi) on hay yield,chemical composition,molecular structures by Fourier transformed infrared(FTIR) spectroscopy,in situ ruminal degradability and in vitro gas production.Seedlings were grown in experimental pots in a greenhouse until first cut and then transferred outside and cut a further 4 times.Biomass yield was similar across the treatments.Acid detergent fiber(ADF) concentration was higher in Pi than in control hay,and ADF decreased further with co-inoculation(P < 0.05).The ether extract(EE) concentration was lower for Pi and Gi+Pi compared with control hay,and control,Pi and Gi+Pi hays had lower EE concentration compared with Sm+Pi(P < 0.05).The FTIR spectroscopic vibration peak height ratio related to proteins(amide1+amide 2): total carbohydrate ratio was lower for the inoculation treatments compared with control hay(P < 0.05).In situ ruminal degradability of dry matter and organic matter were higher for hay of inoculated and co-inoculated seedlings than for control hay(P < 0.05).In conclusion,hay of alfalfa seedlings inoculated and co-inoculated with root growth promoting microorganisms had improved nutritional value compared with hay from non-treated alfalfa seedlings,and co-inoculation was the most effective,however,changes were relatively minor.
