AIM: To studythe effect of indomethacin (IN) on human colon cancer cell line SW480 with p53 mutant and SW480transfected wild-type p53 (wtp53/SW480) in vitro and investigate molecular mechanism of anti-tumor effect of ...AIM: To studythe effect of indomethacin (IN) on human colon cancer cell line SW480 with p53 mutant and SW480transfected wild-type p53 (wtp53/SW480) in vitro and investigate molecular mechanism of anti-tumor effect of IN on colon cancer.METHODS: SW480 cells and wtp53/SW480 cells were treated with different concentrations of IN respectively,the expressions of CDK2, CDK4 and p21WAF1/CIP1 protein were detected by Western blotting.RESULTS: IN gradually down-regulated the expression of CDK2, CDK4 protein of wtp53/SW480 cells in a dosedependent manner, and inhibitory effect reached the maximum level at 600 μmol/L; IN up-regulated the expression of p21WAF1/CIP1 protein in a dose-dependent manner at a certain concentration range, and the expression reached the maximum level at 400 μmol/L,and returned to the base level at 600 μmol/L. The expression of CDK2, CDK4 and p21WAF1/CIP1 protein of SW480cells did not change.CONCLUSION: IN exerts antitumor effect partly through down regulation of the expression of CDK2, CDK4 protein and up regulation of the expression of p21WAF1/PIC1.展开更多
基金Supported by the Ministry of Health, Natural Science Foundation of China, No. 98-2-088
文摘AIM: To studythe effect of indomethacin (IN) on human colon cancer cell line SW480 with p53 mutant and SW480transfected wild-type p53 (wtp53/SW480) in vitro and investigate molecular mechanism of anti-tumor effect of IN on colon cancer.METHODS: SW480 cells and wtp53/SW480 cells were treated with different concentrations of IN respectively,the expressions of CDK2, CDK4 and p21WAF1/CIP1 protein were detected by Western blotting.RESULTS: IN gradually down-regulated the expression of CDK2, CDK4 protein of wtp53/SW480 cells in a dosedependent manner, and inhibitory effect reached the maximum level at 600 μmol/L; IN up-regulated the expression of p21WAF1/CIP1 protein in a dose-dependent manner at a certain concentration range, and the expression reached the maximum level at 400 μmol/L,and returned to the base level at 600 μmol/L. The expression of CDK2, CDK4 and p21WAF1/CIP1 protein of SW480cells did not change.CONCLUSION: IN exerts antitumor effect partly through down regulation of the expression of CDK2, CDK4 protein and up regulation of the expression of p21WAF1/PIC1.
