The existence and stability of stationary solutions for a reaction-diffusion-ODE system are investigated in this paper.We first show that there exist both continuous and discontinuous stationary solutions.Then a good ...The existence and stability of stationary solutions for a reaction-diffusion-ODE system are investigated in this paper.We first show that there exist both continuous and discontinuous stationary solutions.Then a good understanding of the stability of discontinuous stationary solutions is gained under an appropriate condition.In addition,we demonstrate the influences of the diffusion coefficient on stationary solutions.The results we obtained are based on the super-/sub-solution method and the generalized mountain pass theorem.Finally,some numerical simulations are given to illustrate the theoretical results.展开更多
To overcome the disadvantages of current silkworm Bombyx mori transgenic technology, such as costly and time-consuming to maintain non-diapause transgenic silkworms, we report here on the development of treatments for...To overcome the disadvantages of current silkworm Bombyx mori transgenic technology, such as costly and time-consuming to maintain non-diapause transgenic silkworms, we report here on the development of treatments for the germline transformation of diapause silkworm strains. Our results showed that HC1 treatment within 3 h of oviposition was able to prevent the diapause of eggs from Japanese lineage diapause silkworm strains and was also suitable for germline transformation of the same strains. By incu- bating developing mother eggs from Chinese lineage diapause silkworm strains at 15℃ （15℃-IME）, we were able to prevent the diapause of their daughter eggs; a similar strategy （15℃-IMES） for the germline transformation of the same strains was that the mother eggs were incubated at 15℃, and the daughter eggs were then microinjected according to the conventional microinjection methods used for non-diapause eggs. By combining tempera- ture and light controls, the improved 15℃-IMES strategy prevented diapause in daughter eggs, and also enabled the germline transformation of both Japanese and Chinese lineage diapause silkworm strains. Although each of the strategies developed here has advantages and disadvantages, we suggest that the 15℃-IMES strategy is a good reference for the establishment of germline transformation technologies of other egg diapause insects. These new strategies for the efficient germline transformation of diapause silkworm strains are likely to improve the practical use of silkworm transgenic lines in sericulture and also highlight silkworm functional genomics research and its modeling.展开更多
基金supported by National Natural Science Foundation of China(Grant No.11790273,52276028).
文摘The existence and stability of stationary solutions for a reaction-diffusion-ODE system are investigated in this paper.We first show that there exist both continuous and discontinuous stationary solutions.Then a good understanding of the stability of discontinuous stationary solutions is gained under an appropriate condition.In addition,we demonstrate the influences of the diffusion coefficient on stationary solutions.The results we obtained are based on the super-/sub-solution method and the generalized mountain pass theorem.Finally,some numerical simulations are given to illustrate the theoretical results.
文摘To overcome the disadvantages of current silkworm Bombyx mori transgenic technology, such as costly and time-consuming to maintain non-diapause transgenic silkworms, we report here on the development of treatments for the germline transformation of diapause silkworm strains. Our results showed that HC1 treatment within 3 h of oviposition was able to prevent the diapause of eggs from Japanese lineage diapause silkworm strains and was also suitable for germline transformation of the same strains. By incu- bating developing mother eggs from Chinese lineage diapause silkworm strains at 15℃ （15℃-IME）, we were able to prevent the diapause of their daughter eggs; a similar strategy （15℃-IMES） for the germline transformation of the same strains was that the mother eggs were incubated at 15℃, and the daughter eggs were then microinjected according to the conventional microinjection methods used for non-diapause eggs. By combining tempera- ture and light controls, the improved 15℃-IMES strategy prevented diapause in daughter eggs, and also enabled the germline transformation of both Japanese and Chinese lineage diapause silkworm strains. Although each of the strategies developed here has advantages and disadvantages, we suggest that the 15℃-IMES strategy is a good reference for the establishment of germline transformation technologies of other egg diapause insects. These new strategies for the efficient germline transformation of diapause silkworm strains are likely to improve the practical use of silkworm transgenic lines in sericulture and also highlight silkworm functional genomics research and its modeling.
