Foxtail millet(Setaria italica)growth was inhibited because of waterlogging stress,which has caused yield reduc-tion.ERF family plays an important role to plant adversity tolerance.In our study,we obtained 19,819 diff...Foxtail millet(Setaria italica)growth was inhibited because of waterlogging stress,which has caused yield reduc-tion.ERF family plays an important role to plant adversity tolerance.In our study,we obtained 19,819 differential expressed genes(DEGs)between the two treatments based on the RNA-seq sequencing of foxtail millet of water-logging stress.Furthermore,a total of 28 ERF family members were obtained,which have a complete open read-ing frame.We studied the evolution and function of SiERF family and how they affected the waterlogging tolerance.It was found that SiERF1A/B/C(GenBank ID:OR775217,OR775219,OR775218)and SiRAP2-12(GenBank ID:OR775216)have similar functions to the known waterlogging tolerance genes of other plants.Among them,the SiRAP2-12 expression was obviously significantly up-regulated in foxtail millet after 5d water-logging stress.After SiRAP2-12 was silenced,the activity of defense enzymes in millet decreased significantly.In details,superoxide dismutase(SOD),catalase(CAT)and peroxidase(POD),the osmotic regulator proline(Pro),and the activity of the anaerobic respiratory enzyme alcohol dehydrogenase(ADH)content were decreased by 78.61%,29.52%,79.95%,19.41%and 54.77%,respectively.In contrast,the relative electrical conductivity contents(REC),malondialdehyde(MDA),and hydrogen peroxide(H_(2)O_(2))of the foxtail millet subjected to virus-induced gene silencing clearly increased by 1.03-fold,36.09%,and 15.21%,respectively.The content of sodium(Na^(+))in the SiRAP2-12-silenced foxtail millet also increased,but that of potassium(K^(+))decreased.Interestingly,we found that ethylene content was significantly reduced.Further,the SiAOC1 expression,an essential gene for ethylene synthesis,was inhibited in SiRAP2-12-silenced foxtail millet after waterlogging stress.Taken together,we hypothesized that SiRAP2-12 might be a positive regulator of millet tolerance to waterlogging stress.展开更多
Electrolyte interface resistance and low ionic conductivity are essential issues for commercializing solid-state lithium metal batteries(SSLMBs).This work details the fabrication of a double-layer solid composite elec...Electrolyte interface resistance and low ionic conductivity are essential issues for commercializing solid-state lithium metal batteries(SSLMBs).This work details the fabrication of a double-layer solid composite electrolyte(DLSCE)for SSLMBs.The composite comprises poly(vinylidene fluoride-co-hexafluoropropylene)(PVDF-HFP)and poly(methyl methacrylate)(PMMA)combined with 10 wt.%of Li_(6.4)La_(3)Zr_(1.4)Ta_(0.6)O_(12)(LLZTO),synthesized through an ultraviolet curing process.The ionic conductivity of the DLSCE(2.6×10^(-4) S·cm^(-1))at room temperature is the high lithium-ion transference number(0.57),and the tensile strength is 17.8 MPa.When this DLSCE was assembled,the resulted LFP/DLSCE/Li battery exhibited excellent rate performance,with the discharge specific capacities of 162.4,146.9,93.6,and 64.0 mA·h·g^(-1) at 0.1,0.2,0.5,and 1 C,respectively.Furthermore,the DLScE demonstrates remarkable stability with lithium metal batteries,facilitating the stable operation of a Li/Li symmetric battery for over 200 h at both 0.1 and 0.2 mA-cm-2.Notably,the formation of lithium dendrites is also effectively inhibited during cycling.This work provides a novel design strategy and preparation method for solid composite electrolytes.展开更多
In this work, retinal penetration of fluorescein was achieved in vitro by covalent attachment of taurine to fluorescein, yielding the F-Tau conjugate. Nuclear magnetic resonance (NMR) and high resolution mass spectrom...In this work, retinal penetration of fluorescein was achieved in vitro by covalent attachment of taurine to fluorescein, yielding the F-Tau conjugate. Nuclear magnetic resonance (NMR) and high resolution mass spectrometry (HRMS) were used to confirm the successful synthesis of F-Tau. The cellular uptake of F-Tau in adult retinal pigment epithelial cells (ARPE-19) and human retinal microvascular endothelial cells (hRMECs) was visualized via confocal scanning microscopy. The results indicated an improvement of solubility and a reduction of logP of F-Tau compared with fluorescein. As compared with fluorescein, F-Tau showed little toxicity, and was retained longer by cells in uptake experiments. F-Tau also displayed higher transepithelial permeabilities than fluorescein in ARPE-19 and hRMECs monolayer cells (Po0.05). These results showed that taurine may be a useful ligand for targeting small-molecule hydrophobic pharmaceuticals into the retina.展开更多
基金This research was supported by the China Agricultural Research System(CARS-06-14.5-A23)HAAFS Basic Science and Technology Contract Project(Grant No.HBNKY-BGZ-02)Technical System of Foxtail Millet Industry in Hebei Province.
文摘Foxtail millet(Setaria italica)growth was inhibited because of waterlogging stress,which has caused yield reduc-tion.ERF family plays an important role to plant adversity tolerance.In our study,we obtained 19,819 differential expressed genes(DEGs)between the two treatments based on the RNA-seq sequencing of foxtail millet of water-logging stress.Furthermore,a total of 28 ERF family members were obtained,which have a complete open read-ing frame.We studied the evolution and function of SiERF family and how they affected the waterlogging tolerance.It was found that SiERF1A/B/C(GenBank ID:OR775217,OR775219,OR775218)and SiRAP2-12(GenBank ID:OR775216)have similar functions to the known waterlogging tolerance genes of other plants.Among them,the SiRAP2-12 expression was obviously significantly up-regulated in foxtail millet after 5d water-logging stress.After SiRAP2-12 was silenced,the activity of defense enzymes in millet decreased significantly.In details,superoxide dismutase(SOD),catalase(CAT)and peroxidase(POD),the osmotic regulator proline(Pro),and the activity of the anaerobic respiratory enzyme alcohol dehydrogenase(ADH)content were decreased by 78.61%,29.52%,79.95%,19.41%and 54.77%,respectively.In contrast,the relative electrical conductivity contents(REC),malondialdehyde(MDA),and hydrogen peroxide(H_(2)O_(2))of the foxtail millet subjected to virus-induced gene silencing clearly increased by 1.03-fold,36.09%,and 15.21%,respectively.The content of sodium(Na^(+))in the SiRAP2-12-silenced foxtail millet also increased,but that of potassium(K^(+))decreased.Interestingly,we found that ethylene content was significantly reduced.Further,the SiAOC1 expression,an essential gene for ethylene synthesis,was inhibited in SiRAP2-12-silenced foxtail millet after waterlogging stress.Taken together,we hypothesized that SiRAP2-12 might be a positive regulator of millet tolerance to waterlogging stress.
基金supported by the Liuzhou Science and Technology Fund Project(Grant No.2023PRj0103)the National Natural Science Foundation of China(Grant Nos.52161033 and 22262005)+1 种基金the Guangxi Key Laboratory of Automobile Components and Vehicle Technology Fund Project(Grant Nos.2022GKLACVTKF02 and 2023GKLACVTZZ02)the Fund Project of the Key Lab of Guangdong Science and Technology Innovation Strategy Special Fund Project in 2023(Grant No.pdjh2023a0819).
文摘Electrolyte interface resistance and low ionic conductivity are essential issues for commercializing solid-state lithium metal batteries(SSLMBs).This work details the fabrication of a double-layer solid composite electrolyte(DLSCE)for SSLMBs.The composite comprises poly(vinylidene fluoride-co-hexafluoropropylene)(PVDF-HFP)and poly(methyl methacrylate)(PMMA)combined with 10 wt.%of Li_(6.4)La_(3)Zr_(1.4)Ta_(0.6)O_(12)(LLZTO),synthesized through an ultraviolet curing process.The ionic conductivity of the DLSCE(2.6×10^(-4) S·cm^(-1))at room temperature is the high lithium-ion transference number(0.57),and the tensile strength is 17.8 MPa.When this DLSCE was assembled,the resulted LFP/DLSCE/Li battery exhibited excellent rate performance,with the discharge specific capacities of 162.4,146.9,93.6,and 64.0 mA·h·g^(-1) at 0.1,0.2,0.5,and 1 C,respectively.Furthermore,the DLScE demonstrates remarkable stability with lithium metal batteries,facilitating the stable operation of a Li/Li symmetric battery for over 200 h at both 0.1 and 0.2 mA-cm-2.Notably,the formation of lithium dendrites is also effectively inhibited during cycling.This work provides a novel design strategy and preparation method for solid composite electrolytes.
文摘In this work, retinal penetration of fluorescein was achieved in vitro by covalent attachment of taurine to fluorescein, yielding the F-Tau conjugate. Nuclear magnetic resonance (NMR) and high resolution mass spectrometry (HRMS) were used to confirm the successful synthesis of F-Tau. The cellular uptake of F-Tau in adult retinal pigment epithelial cells (ARPE-19) and human retinal microvascular endothelial cells (hRMECs) was visualized via confocal scanning microscopy. The results indicated an improvement of solubility and a reduction of logP of F-Tau compared with fluorescein. As compared with fluorescein, F-Tau showed little toxicity, and was retained longer by cells in uptake experiments. F-Tau also displayed higher transepithelial permeabilities than fluorescein in ARPE-19 and hRMECs monolayer cells (Po0.05). These results showed that taurine may be a useful ligand for targeting small-molecule hydrophobic pharmaceuticals into the retina.