Plant environmental DNA extracted from lacustrine sediments(sedimentary DNA,sedDNA)has been increasingly used to investigate past vegetation changes and human impacts at a high taxonomic resolution.However,the represe...Plant environmental DNA extracted from lacustrine sediments(sedimentary DNA,sedDNA)has been increasingly used to investigate past vegetation changes and human impacts at a high taxonomic resolution.However,the representation of vegetation communities surrounding the lake is still unclear.In this study,we compared plant sedDNA metabarcoding and pollen assemblages from 27 lake surface-sediment samples collected from alpine meadow on the central-eastern Tibetan Plateau to investigate the representation of sedDNA data.In general,the identified components of sedDNA are consistent with the counted pollen taxa and local plant communities.Relative to pollen identification,sedDNA data have higher taxonomic resolution,thus providing a potential approach for reconstructing past plant diversity.The sedDNA signal is strongly influenced by local plants while rarely affected by exogenous plants.Because of the overrepresentation of local plants and PCR bias,the abundance of sedDNA sequence types is very variable among sites,and should be treated with caution when investigating past vegetation cover and climate based on sedDNA data.Our finding suggests that sedDNA analysis can be a complementary approach for investigating the presence/absence of past plants and history of human land-use with higher taxonomic resolution.展开更多
The climate of the North-Central China during the Early Holocene is still debated,particularly with regard to precipitation,despite various paleoclimate records based on multi proxies and models(Chen et al.,2015;Liu J...The climate of the North-Central China during the Early Holocene is still debated,particularly with regard to precipitation,despite various paleoclimate records based on multi proxies and models(Chen et al.,2015;Liu J B et al.,2015;Liu Z Y et al.,2014).The temperature pattern is also uncertain because of a lack of temperature records for north-central China.Pollen sequences are still the most abundant paleo-environmental record particularly for north-central China and have the potential to explore the past precipitation and temperature changes,because both precipitation and temperature are essential for plant growth,and the sensitivity to climatic variables varies among different plants(Liang et al.,2020).Quantitative climate reconstructions based on the modern pollen-climate relationship have been widely generated for east Asia,and the results used to explore the spatial-temporal patterns and mechanisms of past climate changes(Chen et al.,2020,2015;Herzschuh et al.,2019).展开更多
基金supported by the National Natural Science Foundation of China(Grant Nos.42071107 and 41877459)the Mobility program of the Sino-German Center for Research Promotion(No.M-0359)+1 种基金the CAS Pioneer Hundred Talents Program(Xianyong Cao)the Russian Science Foundation(No.20-17-00110).
文摘Plant environmental DNA extracted from lacustrine sediments(sedimentary DNA,sedDNA)has been increasingly used to investigate past vegetation changes and human impacts at a high taxonomic resolution.However,the representation of vegetation communities surrounding the lake is still unclear.In this study,we compared plant sedDNA metabarcoding and pollen assemblages from 27 lake surface-sediment samples collected from alpine meadow on the central-eastern Tibetan Plateau to investigate the representation of sedDNA data.In general,the identified components of sedDNA are consistent with the counted pollen taxa and local plant communities.Relative to pollen identification,sedDNA data have higher taxonomic resolution,thus providing a potential approach for reconstructing past plant diversity.The sedDNA signal is strongly influenced by local plants while rarely affected by exogenous plants.Because of the overrepresentation of local plants and PCR bias,the abundance of sedDNA sequence types is very variable among sites,and should be treated with caution when investigating past vegetation cover and climate based on sedDNA data.Our finding suggests that sedDNA analysis can be a complementary approach for investigating the presence/absence of past plants and history of human land-use with higher taxonomic resolution.
基金supported by the Basic Science Center for Tibetan Plateau Earth System (No.41988101)the National Natural Science Foundation of China (No.41877459)the CAS Pioneer Hundred Talents Program。
文摘The climate of the North-Central China during the Early Holocene is still debated,particularly with regard to precipitation,despite various paleoclimate records based on multi proxies and models(Chen et al.,2015;Liu J B et al.,2015;Liu Z Y et al.,2014).The temperature pattern is also uncertain because of a lack of temperature records for north-central China.Pollen sequences are still the most abundant paleo-environmental record particularly for north-central China and have the potential to explore the past precipitation and temperature changes,because both precipitation and temperature are essential for plant growth,and the sensitivity to climatic variables varies among different plants(Liang et al.,2020).Quantitative climate reconstructions based on the modern pollen-climate relationship have been widely generated for east Asia,and the results used to explore the spatial-temporal patterns and mechanisms of past climate changes(Chen et al.,2020,2015;Herzschuh et al.,2019).
