Ganoderma lucidum is a mushroom widely used for its edible and medicinal properties.Primary bioactive constituents of G.lucidum are ganoderic triterpenoids(GTs),which exhibit important pharmacological activity.Abscisi...Ganoderma lucidum is a mushroom widely used for its edible and medicinal properties.Primary bioactive constituents of G.lucidum are ganoderic triterpenoids(GTs),which exhibit important pharmacological activity.Abscisic acid(ABA),a plant hormone,is associated with plant growth,development,and stress responses.ABA can also affect the growth,metabolism,and physiological activities of different fungi and participates in the regulation of the tetracyclic triterpenes of some plants.Our findings indicated that ABA treatment promoted GT accumulation by regulating the gene expression levels(squalene synthase(sqs),3-hydroxy-3-methylglutaryl-CoA reductase(hmgr),and lanosterol synthase(ls)),and also activated cytosolic Ca2 channels.Furthermore,under ABA mediation.展开更多
Large-scale electrolysis of water to produce high-purity hydrogen is one of the effective ways to solve the energy crisis and environmental pollution problems.However,efficient,cheap and stable catalysts are one of th...Large-scale electrolysis of water to produce high-purity hydrogen is one of the effective ways to solve the energy crisis and environmental pollution problems.However,efficient,cheap and stable catalysts are one of the bottlenecks for industrial application in water splitting.Herein,a facile one-step hydrothermal process was applied to fabricate Mn-doped nickel ferrite nanosheets(Mn-NiFe_(2)O_(4))which shown a low overpotential of 200 mV at 50 mA·cm^(-2)and a small Tafel slope of 47 mV·dec^(-1),together with a prominent turnover frequency(TOF)value(0.14 s^(-1))and robust stability.The in-situ UV-vis spectroscopy unveiled the surface reconstruction to generate NiOOH as active sites during oxygen evolution reaction(OER).The excellent electrocatalytic activity of Mn-NiFe_(2)O_(4)is attributed to the vertically grown nanosheets for exposure more active sites,rich oxygen vacancies,and the hybridization between Ni 3d and O 2p orbitals caused by Mn doping.This work should provide a facile strategy by Mn-doping to simultaneously engineer oxygen vacancies and electronic structure for synergistically triggering oxygen evolution reaction.展开更多
Ganoderic triterpenoids(GTs)are the primary bioactive constituents of the Basidiomycotina fungus,Ganoderma lucidum.These compounds exhibit antitumor,anti-hyperlipidemic,and immune-modulatory pharmacological activities...Ganoderic triterpenoids(GTs)are the primary bioactive constituents of the Basidiomycotina fungus,Ganoderma lucidum.These compounds exhibit antitumor,anti-hyperlipidemic,and immune-modulatory pharmacological activities.This study focused on GT accumulation in mycelia of G.lucidum mediated by the heme oxygenase-1(HO-1)/carbon monoxide(CO)signaling.Compared with the control,hemin(10μmol/L)induced an increase of 60.1%in GT content and 57.1%in HO-1 activity.Moreover,carbon monoxide-releasing molecule-2(CORM-2),CO donor,increased GT content by 56.0%and HO-1 activity by 18.1%.Zn protoporphyrin IX(ZnPPIX),a specific HO-1 inhibitor,significantly reduced GT content by 26.0%and HO-1 activity by 15.8%,while hemin supplementation reversed these effects.Transcriptome sequencing showed that HO-1/CO could function directly as a regulator involved in promoting GT accumulation by regulating gene expression in the mevalonate pathway,and modulating the reactive oxygen species(ROS)and Ca2+pathways.The results of this study may help enhance large-scale GT production and support further exploration of GT metabolic networks and relevant signaling cross-talk.展开更多
基金supported by the Applied Basic Research Project of Shanxi Province(Nos.202203021221135,201901D 211402,and 202103021223255),China.
文摘Ganoderma lucidum is a mushroom widely used for its edible and medicinal properties.Primary bioactive constituents of G.lucidum are ganoderic triterpenoids(GTs),which exhibit important pharmacological activity.Abscisic acid(ABA),a plant hormone,is associated with plant growth,development,and stress responses.ABA can also affect the growth,metabolism,and physiological activities of different fungi and participates in the regulation of the tetracyclic triterpenes of some plants.Our findings indicated that ABA treatment promoted GT accumulation by regulating the gene expression levels(squalene synthase(sqs),3-hydroxy-3-methylglutaryl-CoA reductase(hmgr),and lanosterol synthase(ls)),and also activated cytosolic Ca2 channels.Furthermore,under ABA mediation.
基金We acknowledge the financial support from the National Natural Science Foundation of China(Nos.U1662104 and 21576288).
文摘Large-scale electrolysis of water to produce high-purity hydrogen is one of the effective ways to solve the energy crisis and environmental pollution problems.However,efficient,cheap and stable catalysts are one of the bottlenecks for industrial application in water splitting.Herein,a facile one-step hydrothermal process was applied to fabricate Mn-doped nickel ferrite nanosheets(Mn-NiFe_(2)O_(4))which shown a low overpotential of 200 mV at 50 mA·cm^(-2)and a small Tafel slope of 47 mV·dec^(-1),together with a prominent turnover frequency(TOF)value(0.14 s^(-1))and robust stability.The in-situ UV-vis spectroscopy unveiled the surface reconstruction to generate NiOOH as active sites during oxygen evolution reaction(OER).The excellent electrocatalytic activity of Mn-NiFe_(2)O_(4)is attributed to the vertically grown nanosheets for exposure more active sites,rich oxygen vacancies,and the hybridization between Ni 3d and O 2p orbitals caused by Mn doping.This work should provide a facile strategy by Mn-doping to simultaneously engineer oxygen vacancies and electronic structure for synergistically triggering oxygen evolution reaction.
基金the Applied Basic Research Project of Shanxi Province(No.201901D211402),China。
文摘Ganoderic triterpenoids(GTs)are the primary bioactive constituents of the Basidiomycotina fungus,Ganoderma lucidum.These compounds exhibit antitumor,anti-hyperlipidemic,and immune-modulatory pharmacological activities.This study focused on GT accumulation in mycelia of G.lucidum mediated by the heme oxygenase-1(HO-1)/carbon monoxide(CO)signaling.Compared with the control,hemin(10μmol/L)induced an increase of 60.1%in GT content and 57.1%in HO-1 activity.Moreover,carbon monoxide-releasing molecule-2(CORM-2),CO donor,increased GT content by 56.0%and HO-1 activity by 18.1%.Zn protoporphyrin IX(ZnPPIX),a specific HO-1 inhibitor,significantly reduced GT content by 26.0%and HO-1 activity by 15.8%,while hemin supplementation reversed these effects.Transcriptome sequencing showed that HO-1/CO could function directly as a regulator involved in promoting GT accumulation by regulating gene expression in the mevalonate pathway,and modulating the reactive oxygen species(ROS)and Ca2+pathways.The results of this study may help enhance large-scale GT production and support further exploration of GT metabolic networks and relevant signaling cross-talk.
