目的 探讨溶血三项试验及碳氧血红蛋白(COHb)在胎儿新生儿溶血病(hemolytic disease of the fetus and newborn, HDFN)诊断中的价值。方法 选取2019年1月1日—2022年12月31日于本院新生儿科住院、疑似溶血病并送血清学检查的患儿,其中...目的 探讨溶血三项试验及碳氧血红蛋白(COHb)在胎儿新生儿溶血病(hemolytic disease of the fetus and newborn, HDFN)诊断中的价值。方法 选取2019年1月1日—2022年12月31日于本院新生儿科住院、疑似溶血病并送血清学检查的患儿,其中临床诊断为HDFN的患儿作为溶血组,排除HDFN的患儿作为对照组,对患儿临床及实验室资料进行回顾性收集及分析。COHb水平通过血气分析仪检测获得。结果 溶血组共纳入378例,对照组纳入217例,其中溶血组以足月儿为主(348/378, 92.1%),胎龄39.1(38.3, 40.0)周,354例(354/378,93.7%)为ABO-HDFN,其余为Rh-HDFN;溶血组与对照组间在入院时血清总胆红素、血红蛋白水平、COHb、网织红细胞百分比方面差异有统计学意义(P<0.05)。溶血组溶血三项试验阳性率随日龄增大而降低,2 d内阳性检出率高达80%以上,日龄与COHb之间存在负相关(rs=-0.434,P<0.001)。溶血组释放试验(69.0%)阳性率高于游离试验(55.6%)和直接抗人球蛋白试验(DAT)(36.0%)。建立受试者工作(ROC)曲线,取COHb≥1.15%为最佳截断值,灵敏度51.8%,与溶血三项试验联合并不能显著增加后者对HDFN的诊断效能(Z=-0.727,P>0.05),但其灵敏度高于单项DAT(36.0%)。结论 溶血三项试验对HDFN的诊断有重要价值,释放试验灵敏度最高。COHb对HDFN有一定诊断价值,但联合检测并不能显著增加溶血三项诊断效能。对于存在溶血风险的患儿,生后应尽早送检溶血试验及/或COHb检测。展开更多
Network crewn ether polymer with pendant sulfide side ohain in the networkstructure units has been synthesized via ring- opening copolymerization of β-ethylthioethyl glycidyl ether and diethylene glycol bisglycidyl e...Network crewn ether polymer with pendant sulfide side ohain in the networkstructure units has been synthesized via ring- opening copolymerization of β-ethylthioethyl glycidyl ether and diethylene glycol bisglycidyl ether. A kind of active catalyst suitable for this reaction was suggested. The title polymer was found tobe a good ligand for platinous chloride, and the platinous complex could catalyze thehydrosilylation of olefins with triethoxysilane efficiently.展开更多
Acrylate terpolymer- bound Se, N bidentate ligand was synthesized fromthe side chain chlorine of copolymer and β- dimethylamino -β - hydroxyl diethyl selenoether. The polymer - supported platinum complex exhibited h...Acrylate terpolymer- bound Se, N bidentate ligand was synthesized fromthe side chain chlorine of copolymer and β- dimethylamino -β - hydroxyl diethyl selenoether. The polymer - supported platinum complex exhibited highcatalytic activity in the hodrosilylation of olefins with triethoxysilane.展开更多
目的分析新生儿三尖瓣腱索断裂引起的三尖瓣脱垂合并重度肺动脉高压的临床特点及治疗方法。方法对河北省儿童医院新生儿科2018年11月收治的1例三尖瓣腱索断裂、三尖瓣脱垂合并重度肺动脉高压新生儿的临床资料进行回顾性分析。中文以&qu...目的分析新生儿三尖瓣腱索断裂引起的三尖瓣脱垂合并重度肺动脉高压的临床特点及治疗方法。方法对河北省儿童医院新生儿科2018年11月收治的1例三尖瓣腱索断裂、三尖瓣脱垂合并重度肺动脉高压新生儿的临床资料进行回顾性分析。中文以"新生儿、三尖瓣脱垂、乳头肌或腱索断裂、肺动脉高压"为检索词在中国知网、万方数据库,英文以"(neonate OR newborn) AND (tricuspid valve prolapsed) AND (rupture OR necrosis) AND (papillary muscle OR chordae tendineae) AND (pulmonary hypertension)"为检索式在PubMed数据库中检索自建库至2020年9月报道的临床资料完整的文献,总结该病的临床特点及治疗方法。结果患儿男,9小时龄,因"生后皮肤青紫9 h"入院。患儿系足月剖宫产出生,生后10 min出现全身皮肤发绀、呼吸发憋,出生医院正压通气不能缓解,予气管插管、呼吸机辅助通气,生后9 h病情无好转转至河北省儿童医院。入院查血气分析提示动脉氧分压22.5 mmHg(1 mmHg=0.133 kPa),胸部正位X线片提示双肺透光度正常,超声心动图表现为三尖瓣前叶脱垂、三尖瓣大量反流、重度肺动脉高压、卵圆孔未闭,卵圆孔水平右向左分流。予继续呼吸机辅助呼吸,吸入一氧化氮、静脉用曲前列尼尔联合降肺动脉压治疗,患儿低氧血症逐渐缓解,好转出院。患儿在11月龄时行三尖瓣瓣叶成形术+三尖瓣瓣环成形术+卵圆孔未闭缝合术,术中证实为三尖瓣腱索断裂导致三尖瓣前叶脱垂,随访至22月龄发育状况良好。文献检索未发现中文文献,检索到英文文献12篇,加上本例共分析21例患儿资料,其中仅1例出生胎龄33周早产儿,其余20例均为足月新生儿;21例患儿中男12例。21例患儿均在生后早期出现发绀,接受气管插管机械通气,其他治疗包括吸入一氧化氮、静脉用米力农、血管活性药、利尿剂、前列环素类药物等,6例患儿接受了体外膜氧合治疗。早期报道的2例生后因心肺功能衰竭死亡,其余病例均在接受外科手术后存活,术中或尸检均发现三尖瓣脱垂、三尖瓣乳头肌或腱索断裂。结论乳头肌或腱索断裂导致三尖瓣脱垂在新生儿期罕见,患儿可发生严重低氧血症,早期识别、充分心肺支持稳定血流动力学,适时外科手术可显著降低病死率。展开更多
This study shows that C_(60) can degrade pBR322 plasmid DNA at room temperature without photoactivation. The degradation was enhanced by increasing incubation temperature, reaction time or C_(60) concentration. We als...This study shows that C_(60) can degrade pBR322 plasmid DNA at room temperature without photoactivation. The degradation was enhanced by increasing incubation temperature, reaction time or C_(60) concentration. We also found that superoxide radical anions (O 2 · ) were formed in the C_(60) solution. Superoxide dismutase significantly inhibited DNA cleavage and O 2 · generation induced by C_(60) . These results suggest that DNA cleavage was caused by the formation of reactive oxygen species induced by C_(60) at room temperature. Furthermore, we demonstrate that the DNA degradation was significantly inhibited by acid amide chemicals such as formamide, and by increased ionic strength of the reaction solution. These results indicate that the DNA conformation stability and the surface properties of C_(60) are important factors regulating DNA degradation. We propose that C_(60) can bind DNA, decrease DNA conformation stability, and induce the formation of reactive oxygen species and DNA cleavage at room temperature. Our results provide a possible explanation for the genotoxicity of C_(60) , which should be considered in future use of this particular nano-material.展开更多
文摘目的 探讨溶血三项试验及碳氧血红蛋白(COHb)在胎儿新生儿溶血病(hemolytic disease of the fetus and newborn, HDFN)诊断中的价值。方法 选取2019年1月1日—2022年12月31日于本院新生儿科住院、疑似溶血病并送血清学检查的患儿,其中临床诊断为HDFN的患儿作为溶血组,排除HDFN的患儿作为对照组,对患儿临床及实验室资料进行回顾性收集及分析。COHb水平通过血气分析仪检测获得。结果 溶血组共纳入378例,对照组纳入217例,其中溶血组以足月儿为主(348/378, 92.1%),胎龄39.1(38.3, 40.0)周,354例(354/378,93.7%)为ABO-HDFN,其余为Rh-HDFN;溶血组与对照组间在入院时血清总胆红素、血红蛋白水平、COHb、网织红细胞百分比方面差异有统计学意义(P<0.05)。溶血组溶血三项试验阳性率随日龄增大而降低,2 d内阳性检出率高达80%以上,日龄与COHb之间存在负相关(rs=-0.434,P<0.001)。溶血组释放试验(69.0%)阳性率高于游离试验(55.6%)和直接抗人球蛋白试验(DAT)(36.0%)。建立受试者工作(ROC)曲线,取COHb≥1.15%为最佳截断值,灵敏度51.8%,与溶血三项试验联合并不能显著增加后者对HDFN的诊断效能(Z=-0.727,P>0.05),但其灵敏度高于单项DAT(36.0%)。结论 溶血三项试验对HDFN的诊断有重要价值,释放试验灵敏度最高。COHb对HDFN有一定诊断价值,但联合检测并不能显著增加溶血三项诊断效能。对于存在溶血风险的患儿,生后应尽早送检溶血试验及/或COHb检测。
文摘Network crewn ether polymer with pendant sulfide side ohain in the networkstructure units has been synthesized via ring- opening copolymerization of β-ethylthioethyl glycidyl ether and diethylene glycol bisglycidyl ether. A kind of active catalyst suitable for this reaction was suggested. The title polymer was found tobe a good ligand for platinous chloride, and the platinous complex could catalyze thehydrosilylation of olefins with triethoxysilane efficiently.
文摘Acrylate terpolymer- bound Se, N bidentate ligand was synthesized fromthe side chain chlorine of copolymer and β- dimethylamino -β - hydroxyl diethyl selenoether. The polymer - supported platinum complex exhibited highcatalytic activity in the hodrosilylation of olefins with triethoxysilane.
文摘目的分析新生儿三尖瓣腱索断裂引起的三尖瓣脱垂合并重度肺动脉高压的临床特点及治疗方法。方法对河北省儿童医院新生儿科2018年11月收治的1例三尖瓣腱索断裂、三尖瓣脱垂合并重度肺动脉高压新生儿的临床资料进行回顾性分析。中文以"新生儿、三尖瓣脱垂、乳头肌或腱索断裂、肺动脉高压"为检索词在中国知网、万方数据库,英文以"(neonate OR newborn) AND (tricuspid valve prolapsed) AND (rupture OR necrosis) AND (papillary muscle OR chordae tendineae) AND (pulmonary hypertension)"为检索式在PubMed数据库中检索自建库至2020年9月报道的临床资料完整的文献,总结该病的临床特点及治疗方法。结果患儿男,9小时龄,因"生后皮肤青紫9 h"入院。患儿系足月剖宫产出生,生后10 min出现全身皮肤发绀、呼吸发憋,出生医院正压通气不能缓解,予气管插管、呼吸机辅助通气,生后9 h病情无好转转至河北省儿童医院。入院查血气分析提示动脉氧分压22.5 mmHg(1 mmHg=0.133 kPa),胸部正位X线片提示双肺透光度正常,超声心动图表现为三尖瓣前叶脱垂、三尖瓣大量反流、重度肺动脉高压、卵圆孔未闭,卵圆孔水平右向左分流。予继续呼吸机辅助呼吸,吸入一氧化氮、静脉用曲前列尼尔联合降肺动脉压治疗,患儿低氧血症逐渐缓解,好转出院。患儿在11月龄时行三尖瓣瓣叶成形术+三尖瓣瓣环成形术+卵圆孔未闭缝合术,术中证实为三尖瓣腱索断裂导致三尖瓣前叶脱垂,随访至22月龄发育状况良好。文献检索未发现中文文献,检索到英文文献12篇,加上本例共分析21例患儿资料,其中仅1例出生胎龄33周早产儿,其余20例均为足月新生儿;21例患儿中男12例。21例患儿均在生后早期出现发绀,接受气管插管机械通气,其他治疗包括吸入一氧化氮、静脉用米力农、血管活性药、利尿剂、前列环素类药物等,6例患儿接受了体外膜氧合治疗。早期报道的2例生后因心肺功能衰竭死亡,其余病例均在接受外科手术后存活,术中或尸检均发现三尖瓣脱垂、三尖瓣乳头肌或腱索断裂。结论乳头肌或腱索断裂导致三尖瓣脱垂在新生儿期罕见,患儿可发生严重低氧血症,早期识别、充分心肺支持稳定血流动力学,适时外科手术可显著降低病死率。
基金the National Natural Science Foundation of China (20907017, 20890112)the National Basic Research Program of China (2009CB421605)the National High Technology Research and Development Program of China (2007BAC27B01)
文摘This study shows that C_(60) can degrade pBR322 plasmid DNA at room temperature without photoactivation. The degradation was enhanced by increasing incubation temperature, reaction time or C_(60) concentration. We also found that superoxide radical anions (O 2 · ) were formed in the C_(60) solution. Superoxide dismutase significantly inhibited DNA cleavage and O 2 · generation induced by C_(60) . These results suggest that DNA cleavage was caused by the formation of reactive oxygen species induced by C_(60) at room temperature. Furthermore, we demonstrate that the DNA degradation was significantly inhibited by acid amide chemicals such as formamide, and by increased ionic strength of the reaction solution. These results indicate that the DNA conformation stability and the surface properties of C_(60) are important factors regulating DNA degradation. We propose that C_(60) can bind DNA, decrease DNA conformation stability, and induce the formation of reactive oxygen species and DNA cleavage at room temperature. Our results provide a possible explanation for the genotoxicity of C_(60) , which should be considered in future use of this particular nano-material.
