AIM: To investigate whether Tg737 is regulated by micro RNA-548a-5p(mi R-548a-5p), and correlates with hepatocellular carcinoma(HCC) cell proliferation and apoptosis.METHODS: Assays of loss of function of Tg737 were p...AIM: To investigate whether Tg737 is regulated by micro RNA-548a-5p(mi R-548a-5p), and correlates with hepatocellular carcinoma(HCC) cell proliferation and apoptosis.METHODS: Assays of loss of function of Tg737 were performed by the colony formation assay, CCK assay and cell cycle assay in HCC cell lines. The interaction between mi R-548a-5p and its downstream target, Tg737, was evaluated by a dual-luciferase reporter assay and quantitative real-time polymerase chain reaction. Tg737 was then up-regulated in HCC cells to evaluate its effect on mi R-548a-5p regulation. Hep G2 cells stably overexpressing mi R-548a-5p or mi R-control were also subcutaneously inoculated into nude mice to evaluate the effect of mi R-548a-5p up-regulation on in vivo tumor growth. As the final step, the effect of mi R-548a-5p on the apoptosis induced by cisplatin was evaluated by flow cytometry.RESULTS: Down-regulation of Tg737, which is a target gene of mi R-548a-5p, accelerated HCC cell proliferation, and mi R-548a-5p promoted HCC cell proliferation in vitro and in vivo. Like the downregulation of Tg737, overexpression of mi R-548a-5p in HCC cell lines promoted cell proliferation, increased colony forming ability and hampered cell apoptosis. In addition, mi R-548a-5p overexpression increased HCC cell growth in vivo. Mi R-548a-5p downregulated Tg737 expression through direct contact with its 3' untranslated region(UTR), and mi R-548a-5p expression was negatively correlated with Tg737 levels in HCC specimens. Restoring Tg737(without the 3'UTR) significantly hampered mi R-548a-5p induced cell proliferation, and rescued the mi R-548a-5p induced cell proliferation inhibition and apoptosis induced by cisplatin.CONCLUSION: Mi R-548a-5p negatively regulates the tumor inhibitor gene Tg737 and promotes tumorigenesis in vitro and in vivo, indicating its potential as a novel therapeutic target for HCC.展开更多
评价某高校教职工的个性化健康管理效果。方法采用简单随机抽样方法,选取212名某高校教职工为研究对象,依照其是否参与健康管理分为观察组和对照组,各106人。对照组人群予常规跟踪管理,观察组人群在常规跟踪管理的基础上予健康管理干预...评价某高校教职工的个性化健康管理效果。方法采用简单随机抽样方法,选取212名某高校教职工为研究对象,依照其是否参与健康管理分为观察组和对照组,各106人。对照组人群予常规跟踪管理,观察组人群在常规跟踪管理的基础上予健康管理干预。干预12个月后,比较2组健康管理前后相关生理生化指标的变化情况。结果干预后,与对照组人群比较,观察组人群饮食合理率和运动达标率均升高(61.3% vs 26.4%,52.8% vs 18.9%,P<0.01),体质指数、腰围、舒张压、空腹血糖和血尿酸水平均下降(P<0.05),空腹血糖、腰围、舒张压的异常率均降低(P<0.05)。结论健康管理可有效改善慢性病高危人群的部分健康指标。展开更多
基金Supported by National Natural Science Foundation of ChinaNo.81272648
文摘AIM: To investigate whether Tg737 is regulated by micro RNA-548a-5p(mi R-548a-5p), and correlates with hepatocellular carcinoma(HCC) cell proliferation and apoptosis.METHODS: Assays of loss of function of Tg737 were performed by the colony formation assay, CCK assay and cell cycle assay in HCC cell lines. The interaction between mi R-548a-5p and its downstream target, Tg737, was evaluated by a dual-luciferase reporter assay and quantitative real-time polymerase chain reaction. Tg737 was then up-regulated in HCC cells to evaluate its effect on mi R-548a-5p regulation. Hep G2 cells stably overexpressing mi R-548a-5p or mi R-control were also subcutaneously inoculated into nude mice to evaluate the effect of mi R-548a-5p up-regulation on in vivo tumor growth. As the final step, the effect of mi R-548a-5p on the apoptosis induced by cisplatin was evaluated by flow cytometry.RESULTS: Down-regulation of Tg737, which is a target gene of mi R-548a-5p, accelerated HCC cell proliferation, and mi R-548a-5p promoted HCC cell proliferation in vitro and in vivo. Like the downregulation of Tg737, overexpression of mi R-548a-5p in HCC cell lines promoted cell proliferation, increased colony forming ability and hampered cell apoptosis. In addition, mi R-548a-5p overexpression increased HCC cell growth in vivo. Mi R-548a-5p downregulated Tg737 expression through direct contact with its 3' untranslated region(UTR), and mi R-548a-5p expression was negatively correlated with Tg737 levels in HCC specimens. Restoring Tg737(without the 3'UTR) significantly hampered mi R-548a-5p induced cell proliferation, and rescued the mi R-548a-5p induced cell proliferation inhibition and apoptosis induced by cisplatin.CONCLUSION: Mi R-548a-5p negatively regulates the tumor inhibitor gene Tg737 and promotes tumorigenesis in vitro and in vivo, indicating its potential as a novel therapeutic target for HCC.
文摘评价某高校教职工的个性化健康管理效果。方法采用简单随机抽样方法,选取212名某高校教职工为研究对象,依照其是否参与健康管理分为观察组和对照组,各106人。对照组人群予常规跟踪管理,观察组人群在常规跟踪管理的基础上予健康管理干预。干预12个月后,比较2组健康管理前后相关生理生化指标的变化情况。结果干预后,与对照组人群比较,观察组人群饮食合理率和运动达标率均升高(61.3% vs 26.4%,52.8% vs 18.9%,P<0.01),体质指数、腰围、舒张压、空腹血糖和血尿酸水平均下降(P<0.05),空腹血糖、腰围、舒张压的异常率均降低(P<0.05)。结论健康管理可有效改善慢性病高危人群的部分健康指标。
