Cytoplasmic male sterility exists widely in most natural populations of welsh onion （Alliumfistulosum L.）, which makes it possible to breed out many male sterile lines for heterosis utilization. Unfortunately, the b...Cytoplasmic male sterility exists widely in most natural populations of welsh onion （Alliumfistulosum L.）, which makes it possible to breed out many male sterile lines for heterosis utilization. Unfortunately, the breeding of cytoplasmic male sterility in welsh onion has a little progress due to the limitation of its biological characteristic and traditional selection approach. To study the feasibility and the efficiency of utilizing marker assisted selection for male sterile lines in welsh onion, one SCAR marker, SCS13, and one RAPD marker, S2002400, which could distinguish between N and S cytoplasm in several welsh onion cultivars, were identified. The two markers were then confirmed by Southern blotting, and used to screen the N or S cytoplasm of individual plants in seven welsh onion cultivars in this study. Male sterile and fertile plants were evaluated by aceto-carmine dying. The frequency of N-cytoplasmic plants and maintainer genotype was calculated in the seven open populations of welsh onion. The minimum number of plants needed to identify a maintainer was evaluated to be 95% reliable. Results showed that 20 to 80% decrease of crosses and self-crosses for identifying a maintainer genotype could be achieved by the marker-assisted selection compared with traditional selection method. It was proved that the molecular markers could precisely identify cytoplasmic types individually, performed by one generation of cross and two generations of testcrosses and self-crosses. Finally, several maintainer genotype plants were selected with the help of the two markers in the seven cultivars. The screened markers could assist and accelerate sterile and maintainer lines selection with less labor and cost.展开更多
Several studies have shown a positive relationship between high quality outdoor environments and users’health.Education of agricultural professionals also demands high quality outdoor environments in order to promote...Several studies have shown a positive relationship between high quality outdoor environments and users’health.Education of agricultural professionals also demands high quality outdoor environments in order to promote students’wellbeing,which in turn supports scientific researches.This study analyzed relationship between students’actual uses and campus outdoor environments,identified what environmental characteristics that students prefered,how they used and why they participated in the outdoor activities.The results could be used as guidance of campus design and management.Investigation of three universities in Harbin provided 686 valid questionnaires.The results suggested that health awareness effectively promoted students’use frequencies of outdoor environments.Environment with"natural"and"cultural"characteristics,especially undulating landform,vibrant plants and landmarks in a serpentins design style attracted more uses.Female students showed more preferences on social interaction activities than males.Students who had good economic consumption levels and participated in outdoor activities frequntly in childhood prefered to join all the kinds of activities after university enrollment.展开更多
目的合成一系列嘧啶联苯类化合物,测试其对8-羟基鸟嘌呤核苷酸酶(Mut T homolog 1,MTH1酶)和癌细胞增殖的体外抑制活性。方法以对溴硝基苯和二氯嘧啶为起始原料,经Suzuki偶联、铁酸还原和取代等反应得到4个目标化合物。酶实验根据MTH1...目的合成一系列嘧啶联苯类化合物,测试其对8-羟基鸟嘌呤核苷酸酶(Mut T homolog 1,MTH1酶)和癌细胞增殖的体外抑制活性。方法以对溴硝基苯和二氯嘧啶为起始原料,经Suzuki偶联、铁酸还原和取代等反应得到4个目标化合物。酶实验根据MTH1酶水解d GTP的特性,结合分光光度法,计算酶抑制率。细胞实验选用SW480和Hep G2细胞株,采用MTT法测定。结果与结论化合物6a-1、6a-2、6a-3、4b具有体外MTH1酶抑制活性,化合物3a、5a-1、5a-3、6a-1、6a-2、6a-3、3b、4b具有一定癌细胞增殖抑制活性。展开更多
基金supported by the National Natural Science Foundation of China (39770520)
文摘Cytoplasmic male sterility exists widely in most natural populations of welsh onion （Alliumfistulosum L.）, which makes it possible to breed out many male sterile lines for heterosis utilization. Unfortunately, the breeding of cytoplasmic male sterility in welsh onion has a little progress due to the limitation of its biological characteristic and traditional selection approach. To study the feasibility and the efficiency of utilizing marker assisted selection for male sterile lines in welsh onion, one SCAR marker, SCS13, and one RAPD marker, S2002400, which could distinguish between N and S cytoplasm in several welsh onion cultivars, were identified. The two markers were then confirmed by Southern blotting, and used to screen the N or S cytoplasm of individual plants in seven welsh onion cultivars in this study. Male sterile and fertile plants were evaluated by aceto-carmine dying. The frequency of N-cytoplasmic plants and maintainer genotype was calculated in the seven open populations of welsh onion. The minimum number of plants needed to identify a maintainer was evaluated to be 95% reliable. Results showed that 20 to 80% decrease of crosses and self-crosses for identifying a maintainer genotype could be achieved by the marker-assisted selection compared with traditional selection method. It was proved that the molecular markers could precisely identify cytoplasmic types individually, performed by one generation of cross and two generations of testcrosses and self-crosses. Finally, several maintainer genotype plants were selected with the help of the two markers in the seven cultivars. The screened markers could assist and accelerate sterile and maintainer lines selection with less labor and cost.
基金Supported by the Natural Fund Project of Heilongjiang Province(E2018009)Heilongjiang Higher Education Teaching Reform Project(SJGY20170111)Great Northern Agricultural Scholar Program(SBJY 17024)。
文摘Several studies have shown a positive relationship between high quality outdoor environments and users’health.Education of agricultural professionals also demands high quality outdoor environments in order to promote students’wellbeing,which in turn supports scientific researches.This study analyzed relationship between students’actual uses and campus outdoor environments,identified what environmental characteristics that students prefered,how they used and why they participated in the outdoor activities.The results could be used as guidance of campus design and management.Investigation of three universities in Harbin provided 686 valid questionnaires.The results suggested that health awareness effectively promoted students’use frequencies of outdoor environments.Environment with"natural"and"cultural"characteristics,especially undulating landform,vibrant plants and landmarks in a serpentins design style attracted more uses.Female students showed more preferences on social interaction activities than males.Students who had good economic consumption levels and participated in outdoor activities frequntly in childhood prefered to join all the kinds of activities after university enrollment.
