LAMP2(lysosome associated membrane glycoprotein 2)is an important autophagy protein but its role in estrogenic regulation on spatial learning and memory remains unclear.In this study,we first infused aromatase(estroge...LAMP2(lysosome associated membrane glycoprotein 2)is an important autophagy protein but its role in estrogenic regulation on spatial learning and memory remains unclear.In this study,we first infused aromatase(estrogen synthase)-specific RNA interference AAV into the hippocampus of mice,label-free quantitative proteomics analysis revealed that LAMP2 was the most significantly upregulated protein.This upregulation was further proved with RT-PCR,WB,IHC in ovariectomy(OVX)mice.Then,LAMP2-overexpressing(oLAMP2)and RNA interference(shLAMP2)virus vectors were injected into the hippocampus of OVX mice,respectively.展开更多
文摘LAMP2(lysosome associated membrane glycoprotein 2)is an important autophagy protein but its role in estrogenic regulation on spatial learning and memory remains unclear.In this study,we first infused aromatase(estrogen synthase)-specific RNA interference AAV into the hippocampus of mice,label-free quantitative proteomics analysis revealed that LAMP2 was the most significantly upregulated protein.This upregulation was further proved with RT-PCR,WB,IHC in ovariectomy(OVX)mice.Then,LAMP2-overexpressing(oLAMP2)and RNA interference(shLAMP2)virus vectors were injected into the hippocampus of OVX mice,respectively.
