Diabetic kidney disease(DKD)is the most serious microvascular complication during the development of diabetes with the characterizations of glomerular basement membrane thickening,mesangial expansion,and glomerular sc...Diabetic kidney disease(DKD)is the most serious microvascular complication during the development of diabetes with the characterizations of glomerular basement membrane thickening,mesangial expansion,and glomerular sclerosis,eventually leading to end-stage renal disease.This study aimed to investigate the melioration effect of Codonopisis tangshen Oliv.(COD)on the DKD model,which was established by unilateral nephrectomy(UN)-high fat diet feeding(HFD)combined with streptozotocin(STZ).After the DKD rats were oral treated with COD at a dose of 2.7 mg/kg for 4 consecutive weeks,the blood glucose,lipid metabolism,renal function,inflammatory mediators,and fibrosis-associated proteins were examined.In vivo,the COD administration obviously relieved the weight loss,water intake,and blood glucose;decreased the total cholesterol,triglyceride,and low-density lipoprotein cholesterol levels;and improved the renal function by reducing the expression of serum creatinine,uric acid,and urinary protein compared with the model group.The levels of pro-inflammatory cytokines of tumor necrosis factor-a,interleukin-1β,and IL-6 were significantly inhibited by COD.Meanwhile,the deposition of collagen fiber was markedly increased,and the protein and mRNA expressions of transforming growth factor-b1 and a-smooth muscle actin were markedly elevated in DKD rats,but they were decreased to some extent after the COD treatment.In conclusion,COD exhibited a protective effect on the UN-HFD feeding combined with STZ-induced DKD model by improving the blood glucose and lipid metabolism,relieving the inflammatory response,and mitigating the renal fibrosis,which provided scientific evidence for its applications in clinic.展开更多
Since 2019,an epidemic of pneumonia of unknown cause occurred around the world.[1]The analysis of respiratory tract samples led to the identification of a novel coronavirus,which was named 2019 novel coronavirus(2019-...Since 2019,an epidemic of pneumonia of unknown cause occurred around the world.[1]The analysis of respiratory tract samples led to the identification of a novel coronavirus,which was named 2019 novel coronavirus(2019-nCoV).Epidemiological data showed that 2019-nCoV could spread rapidly from person to person and cause coronavirus disease-19(COVID-19).The high infectivity of COVID-19 has led to a rapid increase in the number of cases and caused a global pandemic.To date,2019-nCoV has infected millions of people and caused hundreds of thousands of deaths worldwide.These numbers are updated every day and are expected to increase further at a great rate.COVID-19 has now become a serious threat to public health worldwide.展开更多
基金This research was funded by the National Natural Science Foundation of China Grant Numbers 81503376 and 81774213Applied Science and Technology Research Foundation of Guangdong Province Grant Number 2016B020237005+1 种基金College Students Entrepreneurship Training Program of Southern Medical University(201712121083)Scientific Enlightenment Program of Southern Medical University(b1000501).
文摘Diabetic kidney disease(DKD)is the most serious microvascular complication during the development of diabetes with the characterizations of glomerular basement membrane thickening,mesangial expansion,and glomerular sclerosis,eventually leading to end-stage renal disease.This study aimed to investigate the melioration effect of Codonopisis tangshen Oliv.(COD)on the DKD model,which was established by unilateral nephrectomy(UN)-high fat diet feeding(HFD)combined with streptozotocin(STZ).After the DKD rats were oral treated with COD at a dose of 2.7 mg/kg for 4 consecutive weeks,the blood glucose,lipid metabolism,renal function,inflammatory mediators,and fibrosis-associated proteins were examined.In vivo,the COD administration obviously relieved the weight loss,water intake,and blood glucose;decreased the total cholesterol,triglyceride,and low-density lipoprotein cholesterol levels;and improved the renal function by reducing the expression of serum creatinine,uric acid,and urinary protein compared with the model group.The levels of pro-inflammatory cytokines of tumor necrosis factor-a,interleukin-1β,and IL-6 were significantly inhibited by COD.Meanwhile,the deposition of collagen fiber was markedly increased,and the protein and mRNA expressions of transforming growth factor-b1 and a-smooth muscle actin were markedly elevated in DKD rats,but they were decreased to some extent after the COD treatment.In conclusion,COD exhibited a protective effect on the UN-HFD feeding combined with STZ-induced DKD model by improving the blood glucose and lipid metabolism,relieving the inflammatory response,and mitigating the renal fibrosis,which provided scientific evidence for its applications in clinic.
文摘Since 2019,an epidemic of pneumonia of unknown cause occurred around the world.[1]The analysis of respiratory tract samples led to the identification of a novel coronavirus,which was named 2019 novel coronavirus(2019-nCoV).Epidemiological data showed that 2019-nCoV could spread rapidly from person to person and cause coronavirus disease-19(COVID-19).The high infectivity of COVID-19 has led to a rapid increase in the number of cases and caused a global pandemic.To date,2019-nCoV has infected millions of people and caused hundreds of thousands of deaths worldwide.These numbers are updated every day and are expected to increase further at a great rate.COVID-19 has now become a serious threat to public health worldwide.
