The accumulation of excessive reactive oxygen species can exacerbate any injury of retinal tissue because free radicals can trigger lipid peroxidation,protein damage and DNA fragmentation.Increased oxidative stress is...The accumulation of excessive reactive oxygen species can exacerbate any injury of retinal tissue because free radicals can trigger lipid peroxidation,protein damage and DNA fragmentation.Increased oxidative stress is associated with the common pathological process of many eye diseases,such as glaucoma,diabetic retinopathy and ischemic optic neuropathy.Many studies have demonstrated that Lycium barbarum polysaccharides(LBP)protects against oxidative injury in numerous cells and tissues.For the model of hypoxia we used cultured retinal ganglion cells and induced hypoxia by incubating with 200μM cobalt chloride(CoCl2)for 24 hours.To investigate the protective effect of LBP and its mechanism of action against oxidative stress injury,the retinal tissue was pretreated with 0.5 mg/mL LBP for 24 hours.The results of flow cytometric analysis showed LBP could effectively reduce the CoCl2-induced retinal ganglion cell apoptosis,inhibited the generation of reactive oxygen species and the reduction of mitochondrial membrane potential.These findings suggested that LBP could protect retinal ganglion cells from CoCl2-induced apoptosis by reducing mitochondrial membrane potential and reactive oxygen species.展开更多
We used discrete dipole approximation(DDA)to examine the scattering and absorption characteristics of spherical ice crystal particles.On this basis,we studied the scattering characteristics of spherical ice crystal pa...We used discrete dipole approximation(DDA)to examine the scattering and absorption characteristics of spherical ice crystal particles.On this basis,we studied the scattering characteristics of spherical ice crystal particles at different frequencies and non-spherical ice crystal particles with different shapes,aspect ratios,and spatial orientations.The results indicate that the DDA and Mie methods yield almost the same results for spherical ice crystal particles,illustrating the superior calculation accuracy of the DDA method.Compared with the millimeter wave band,the terahertz band particles have richer scattering characteristics and can detect ice crystal particles more easily.Different frequencies,shapes,aspect ratios,and spatial orientations have specific effects on the scattering and absorption characteristics o f ice crystal particles.The results provide an important theoretical basis for the design of terahertz cloud radars and related cirrus detection methods.展开更多
基金supported by grants from Project of Administration of Traditional Chinese Medicine of Guangdong Province of China,No.20161071(to LL)Medical Scientific Research Foundation of Guangdong Province of China,No.A2019098(to LL)
文摘The accumulation of excessive reactive oxygen species can exacerbate any injury of retinal tissue because free radicals can trigger lipid peroxidation,protein damage and DNA fragmentation.Increased oxidative stress is associated with the common pathological process of many eye diseases,such as glaucoma,diabetic retinopathy and ischemic optic neuropathy.Many studies have demonstrated that Lycium barbarum polysaccharides(LBP)protects against oxidative injury in numerous cells and tissues.For the model of hypoxia we used cultured retinal ganglion cells and induced hypoxia by incubating with 200μM cobalt chloride(CoCl2)for 24 hours.To investigate the protective effect of LBP and its mechanism of action against oxidative stress injury,the retinal tissue was pretreated with 0.5 mg/mL LBP for 24 hours.The results of flow cytometric analysis showed LBP could effectively reduce the CoCl2-induced retinal ganglion cell apoptosis,inhibited the generation of reactive oxygen species and the reduction of mitochondrial membrane potential.These findings suggested that LBP could protect retinal ganglion cells from CoCl2-induced apoptosis by reducing mitochondrial membrane potential and reactive oxygen species.
基金Project supported by the National Natural Science Foundation of China(Grant Nos.61527805 and 41776181).
文摘We used discrete dipole approximation(DDA)to examine the scattering and absorption characteristics of spherical ice crystal particles.On this basis,we studied the scattering characteristics of spherical ice crystal particles at different frequencies and non-spherical ice crystal particles with different shapes,aspect ratios,and spatial orientations.The results indicate that the DDA and Mie methods yield almost the same results for spherical ice crystal particles,illustrating the superior calculation accuracy of the DDA method.Compared with the millimeter wave band,the terahertz band particles have richer scattering characteristics and can detect ice crystal particles more easily.Different frequencies,shapes,aspect ratios,and spatial orientations have specific effects on the scattering and absorption characteristics o f ice crystal particles.The results provide an important theoretical basis for the design of terahertz cloud radars and related cirrus detection methods.
