Deciphering the population structure and genetic basis of growth traits from whole-genome resequencing of the leopard coral grouper(Plectropomus leopardus)

The leopard coral grouper(Plectropomus leopardus)is a species of significant economic importance.Although artificial cultivation of P.leopardus has thrived in recent decades,the advancement of selective breeding has been hindered by the lack of comprehensive population genomic data.In this study,we identified over 8.73 million single nucleotide polymorphisms(SNPs)through whole-genome resequencing of 326 individuals spanning six distinct groups.Furthermore,we categorized 226 individuals with high-coverage sequencing depth(≥14×)into eight clusters based on their genetic profiles and phylogenetic relationships.Notably,four of these clusters exhibited pronounced genetic differentiation compared with the other populations.To identify potentially advantageous loci for P.leopardus,we examined genomic regions exhibiting selective sweeps by analyzing the nucleotide diversity(θπ)and fixation index(FST)in these four clusters.Using these high-coverage resequencing data,we successfully constructed the first haplotype reference panel specific to P.leopardus.This achievement holds promise for enabling high-quality,cost-effectiveimputationmethods.Additionally,we combined low-coverage sequencing data with imputation techniques for a genome-wide association study,aiming to identify candidate SNP loci and genes associated with growth traits.A significant concentration of these genes was observed on chromosome 17,which is primarily involved in skeletal muscle and embryonic development and cell proliferation.Notably,our detailed investigation of growth-related SNPs across the eight clusters revealed that cluster 5 harbored the most promising candidate SNPs,showing potential for genetic selective breeding efforts.These findings provide a robust toolkit and valuable insights into the management of germplasm resources and genome-driven breeding initiatives targeting P.leopardus.

Study on the thermal deformation behavior and microstructure of FGH96 heat extrusion alloy during two-pass hot deformation

The change rules associated with hot deformation of FGH96 alloy were investigated by isothermal two-pass hot deformation tests in the temperature range 1050–1125°C and at strain rates ranging from 0.001 to 0.1 s^(-1) on a Gleeble 3500 thermo-simulation machine. The results showed that the softening degree of the alloy between passes decreases with increasing temperature and decreasing strain rates. The critical strain of the first-pass is greater than that of the second-pass. The true stress–true strain curves showed that single-peak dynamic recrystallization, multi-peak dynamic recrystallization, and dynamic response occur when the strain rate is 0.1, 0.01, and 0.001 s^(-1), respectively. The alloy contains three different grain structures after hot deformation: partially recrystallized tissue, completely fine recrystallized tissue, coarse-grained grains. The small-angle grain boundaries increase with increasing temperature. Increasing strain rates cause the small-angle grain boundaries to first increase and then decrease.

Preparation and radar-absorbing properties of Al2O3/TiO2/Fe2O3/Yb2O3 composite powder

Al2O3/TiOe/FeeO3/Yb2O3 composite powder was synthesized via the sol-gel method. The structure, morphology, and ra- dar-absorption properties of the composite powder were characterized by transmission electron microscopy, X-ray diffraction analysis and RF impedance analysis. The results show that two types of particles exist in the composite powder. One is irregular flakes （100-200 rim） and the other is spherical A1203 particles （smaller than 80 rim）. Electromagnetic wave attenuation is mostly achieved by dielectric loss. The maximum value of the dissipation factor reaches 0.76 （at 15.68 GHz） in the frequency range of 2-18 GHz. The electromagnetic absorption of waves covers 2-18 GHz with the matching thicknesses of 1.5-4.5 mm. The absorption peak shifts to the lower-frequency area with increas- ing matching thickness. The effective absorption hand covers the frequency range of 2.16-9.76 GHz, and the maximum absorption peak reaches -20.18 dB with a matching thickness of 3.5 mm at a frequency of 3.52 GHz.

Association between Mycoplasma pneumoniae infection and acute coronary syndrome

Objective: To investigate the association between Mycoplasma pneumoniae (MP) infection and the occurrence of acute coronary syndromes (ACS), and its associated mechanism in ACS development. Methods: A total of 134 patients with confirmed ACS were selected as the ACS group, and another 102 healthy subjects were enrolled as the control group. Serum triglycerides (TG), cholesterol (TC), high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C), apolipoprotein A1 (ApoA1), and apolipoprotein B (ApoB) were detected using immuno-transmission turbidimetry in the ACS and control groups. An enzyme-linked immunosorbent assay was used to detect MP-specific IgG antibodies in the serum. Results:The MP infection rate in the ACS group was significantly higher than that in of the healthy control group. Although there were significant differences in the expression of TC, HDL, LDL, and ApoB between the ACS and control groups, there were no significant differences between the MP IgG-positive and negative groups for any the six serum lipid indexes in the ACS patients. The level of high-sensitivity C-reactive protein (hs-CRP) expression in ACS patients was significantly higher in the MP IgG-positive group compared with the negative group. Conclusions: MP infection is associated with ACS and may be a risk factor for ACS. MP infection may not affect blood lipid levels but rather induce the development of ACS by affecting the long-term inflammatory environment.