Periodontitis is a type of chronic inflammation in the gingival tissue caused by infectious bacteria colonizing the surface of the teeth,leading to the destruction of tooth-supporting tissues and loss of alveolar bone...Periodontitis is a type of chronic inflammation in the gingival tissue caused by infectious bacteria colonizing the surface of the teeth,leading to the destruction of tooth-supporting tissues and loss of alveolar bone.Suberoylanilide hydroxamic acid(SAHA),a class of histone deacetylase(HDAC)inhibitor,has the potential to stimulate osteoblast differentiation by acetylating histone proteins,and thus suppressing the expression of adipogenic and chondrogenic genes.However,the effect of SAHA on the differentiation of human periodontal ligament stem cells(hPDLSCs)is yet to be elucidated.Herein,we investigated the effects of SAHA on in vitro proliferation and differentiation of hPDLSCs by MTT assay,Alizarin Red-S,and alkaline phosphatase staining,and real-time PCR.Notably,300 ng/mL SAHA treatment enhanced the proliferation and mineralization of hPDLSCs,indicating their osteogenic potential.Moreover,a significant enhancement of osteogenesis gene markers and proteins was observed.We also demonstrated that ERK is a positive regulator of Runx2 transcription factors during osteoblast differentiation.These results indicate that SAHA may be a useful osteogenic induction agent for periodontal bone regeneration.展开更多
Gaining a thorough understanding of the theoretical principles of rock breaking with a disc cutter is a critical issue in tunnel boring machine(TBM) technology.To fully consider the complexity and importance of the ba...Gaining a thorough understanding of the theoretical principles of rock breaking with a disc cutter is a critical issue in tunnel boring machine(TBM) technology.To fully consider the complexity and importance of the basic principles of rock breaking during tunnel excavation,in this paper we use a new method, the smooth particle hydrodynamics(SPH), to study the rock-breaking mechanism and verify its accuracy and feasibility. Using the SPH method, we induce the rock fragmentation process with two cutters in synchronous and sequential orders. The results show that when the cutters act on rock sequentially,the second indentation influences the crack evolution of the first indentation. With increased cutter spacing, the second crack gradually becomes independent of the first crack.Under synchronous action of the two cutters, a bursiform nucleus is generated beneath the cutters and the area of the nucleus increases with increased cutter spacing. Whether the cutters act on the rock sequentially or synchronously,we found the optimum cutter spacing of our chosen rock type to be 60 mm. Our analyses results show that the efficiency of sequential rock cutting is superior to synchronous cutting, both with respect to crack evolution and cutter force.展开更多
Pancreatic ductal adenocarcinoma(PDAC)is universally acknowledged as the cancer with the highest mortality rate.Berberine has high medicinal value and has been used as an anti-cancer agent.Hence the purpose of this st...Pancreatic ductal adenocarcinoma(PDAC)is universally acknowledged as the cancer with the highest mortality rate.Berberine has high medicinal value and has been used as an anti-cancer agent.Hence the purpose of this study was to investigate the anti-cancer effect of berberine in PDAC.Berberine was shown to have a selective anti-cancer effect on PDAC by MTT assay in vitro.Pancreatic cancer stem cells(PCSCs),regulated by epithelial-mesenchymal transition(EMT),could promote the proliferation of PDAC cells.However,berberine suppressed the proliferation and stemness of PCSCs through immunofluorescence staining,stem cell sphere assays and so forth in vitro.In vivo,berberine reduced tumor size and decreased the expression levels of Ki67,a marker of cellular proliferation,in orthotopic pancreatic tumors.In addition,berberine inhibited the EMT signaling pathway by RT-PCR and Western blotting methods both in vitro and in vivo.Our study indicates that berberine inhibits the proliferation of PDAC cells both in vivo and in vitro.The mechanism of the anti-cancer effect of berberine likely involves the inhibition of EMT.Therefore,berberine may be a novel antineoplastic drug with clinical efficacy in PDAC.展开更多
Maintaining the stemness of the transplanted stem cell spheroids in an inflammatory microenvironment is challenging but important in regenerative medicine. Direct delivery of stem cells to repair periodontal defects m...Maintaining the stemness of the transplanted stem cell spheroids in an inflammatory microenvironment is challenging but important in regenerative medicine. Direct delivery of stem cells to repair periodontal defects may yield suboptimal effects due to the complexity of the periodontal inflammatory environment. Herein, stem cell spheroid is encapsulated by interfacial assembly of metal-phenolic network(MPN) nanofilm to form a stem cell microsphere capsule. Specifically, periodontal ligament stem cells(PDLSCs) spheroid was coated with FeⅢ/tannic acid coordination network to obtain spheroid@[FeⅢ-TA] microcapsules. The formed biodegradable MPN biointerface acted as a cytoprotective barrier and exhibited antioxidative, antibacterial and anti-inflammatory activities, effectively remodeling the inflammatory microenvironment and maintaining the stemness of PDLSCs. The stem cell microencapsulation proposed in this study can be applied to multiple stem cells with various functional metal ion/polyphenol coordination, providing a simple yet efficient delivery strategy for stem cell stemness maintenance in an inflammatory environment toward a better therapeutic outcome.展开更多
基金supported by the scientific research project of Baishanzu National Park(2023JBGS07)the National Natural Science Foundation of China(31970447,32370500)+1 种基金the starting fund for doctoral research of Lishui University(6004LMM01Z)special funds for Scientific Research of Postdoctoral Work Station Assessment in Zhejiang Province,China(2023).
基金partly supported by the scientific research project of Baishanzu National Park(2021KFLY08)the National Natural Science Foundation of China(31970447)+1 种基金the Starting Fund for Doctoral Research of Lishui University(6004LMM01Z)the Special Fund for Scientific Research of Postdoctoral Work Station Assessment in Zhejiang Province,China(2021,2023)。
基金supported by the starting fund for doctoral research of Lishui University(6004LMM01Z)the National Natural Science Foundation of China(31970447)+1 种基金the Scientific Research Project of Baishanzu National Park(2021KFLY08)special funds for scientific research of postdoctoral work station assessment of Lishui City(2021,2023)。
基金partly supported by the starting fund for doctoral research of Lishui University(6004LMM01Z)the National Natural Science Foundation of China(31970447)+1 种基金the Scientific Research Project of Baishanzu National Park(2021KFLY08)Special Fund for Scientific Research of Postdoctoral Work Station Assessment in Zhejiang Province,China(2021,2023)。
基金partly supported by the Natural Science Foundation of Zhejiang Province (LY18C040001)the National Natural Science Foundation of China (31672344,31501885)
文摘记述中国安徽和江西境内钩瓣叶蜂属Macrophya Dahlbom女贞钩瓣叶蜂种团M.ligustri group 2新种:官山钩瓣叶蜂M.guanshanicus Li,Liu&Wei sp.nov.和黑角钩瓣叶蜂M.nigroscapila Li,Liu&Wei sp.nov.。
基金partly supported by the Natural Science Foundation of Zhejiang Province (LY18C040001)the National Natural Science Foundation of China (31672344, 31501885)+1 种基金the open fund from Key Laboratory of Insect Evolution and Pest Management for Higher Education in Hunan Province (010211)Central South University of Forestry and Technology
基金partly supported by the Natural Science Foundation of Zhejiang Province(LY18C040001)the National Natural Science Foundation of China (31672344,31501885)the open fund from Common College Key Laboratory of Insect Systematic Evolution and Integrated Management of Hunan(010211),Central South University of Forestry and Technology
基金This research was partly supported by the Zhejiang Provincial Natural Science Foundation of China(LY18C040001)the National Natural Science Foundation of China(31672344).
基金the National Research Foundation of Korea(NRF)and funded by the Ministry of Education(No.2018R1A6A1A03025582)the National Research Foundation of Korea(NRF-2019R1D1A3A03103828).
文摘Periodontitis is a type of chronic inflammation in the gingival tissue caused by infectious bacteria colonizing the surface of the teeth,leading to the destruction of tooth-supporting tissues and loss of alveolar bone.Suberoylanilide hydroxamic acid(SAHA),a class of histone deacetylase(HDAC)inhibitor,has the potential to stimulate osteoblast differentiation by acetylating histone proteins,and thus suppressing the expression of adipogenic and chondrogenic genes.However,the effect of SAHA on the differentiation of human periodontal ligament stem cells(hPDLSCs)is yet to be elucidated.Herein,we investigated the effects of SAHA on in vitro proliferation and differentiation of hPDLSCs by MTT assay,Alizarin Red-S,and alkaline phosphatase staining,and real-time PCR.Notably,300 ng/mL SAHA treatment enhanced the proliferation and mineralization of hPDLSCs,indicating their osteogenic potential.Moreover,a significant enhancement of osteogenesis gene markers and proteins was observed.We also demonstrated that ERK is a positive regulator of Runx2 transcription factors during osteoblast differentiation.These results indicate that SAHA may be a useful osteogenic induction agent for periodontal bone regeneration.
基金supported by the National Natural Science Foundation of China (No. 51275339, No. 51675374 and No. 5157051735)
文摘Gaining a thorough understanding of the theoretical principles of rock breaking with a disc cutter is a critical issue in tunnel boring machine(TBM) technology.To fully consider the complexity and importance of the basic principles of rock breaking during tunnel excavation,in this paper we use a new method, the smooth particle hydrodynamics(SPH), to study the rock-breaking mechanism and verify its accuracy and feasibility. Using the SPH method, we induce the rock fragmentation process with two cutters in synchronous and sequential orders. The results show that when the cutters act on rock sequentially,the second indentation influences the crack evolution of the first indentation. With increased cutter spacing, the second crack gradually becomes independent of the first crack.Under synchronous action of the two cutters, a bursiform nucleus is generated beneath the cutters and the area of the nucleus increases with increased cutter spacing. Whether the cutters act on the rock sequentially or synchronously,we found the optimum cutter spacing of our chosen rock type to be 60 mm. Our analyses results show that the efficiency of sequential rock cutting is superior to synchronous cutting, both with respect to crack evolution and cutter force.
基金This research was supported by 81803024(National Natural Science Foundation of China)and DUT21LK23(Fundamental Research Funds for the Central Universities).
文摘Pancreatic ductal adenocarcinoma(PDAC)is universally acknowledged as the cancer with the highest mortality rate.Berberine has high medicinal value and has been used as an anti-cancer agent.Hence the purpose of this study was to investigate the anti-cancer effect of berberine in PDAC.Berberine was shown to have a selective anti-cancer effect on PDAC by MTT assay in vitro.Pancreatic cancer stem cells(PCSCs),regulated by epithelial-mesenchymal transition(EMT),could promote the proliferation of PDAC cells.However,berberine suppressed the proliferation and stemness of PCSCs through immunofluorescence staining,stem cell sphere assays and so forth in vitro.In vivo,berberine reduced tumor size and decreased the expression levels of Ki67,a marker of cellular proliferation,in orthotopic pancreatic tumors.In addition,berberine inhibited the EMT signaling pathway by RT-PCR and Western blotting methods both in vitro and in vivo.Our study indicates that berberine inhibits the proliferation of PDAC cells both in vivo and in vitro.The mechanism of the anti-cancer effect of berberine likely involves the inhibition of EMT.Therefore,berberine may be a novel antineoplastic drug with clinical efficacy in PDAC.
基金supported by the National Natural Science Foundation of China(Nos.82100977,82170964,81873716,and 82001042)China Postdoctoral Science Foundation(2021M691930)+7 种基金Shandong Province Key Research and Development Program(No.2021ZDSYS18)Shandong Province Major Scientific and Technical Innovation Project(No.2021SFGC0502)the Youth Scientific Research Funds of School of Stomatology,Shandong University(2020QNJJ03)the Opening Research Fund from Key Laboratory of Shaanxi Province for Craniofacial Precision Medicine Research,College of Stomatology,Xi’an Jiaotong University(2022LHM-KFKT004)the Construction Engineering Special Fund of“Taishan Scholars”of Shandong Province(No.ts20190975 and tsqn201909180)Collaborative Innovation Center of Technology and Equipment for Biological Diagnosis,Therapy in Universities of Shandong,Rongxiang Regenerative Medicine Foundation of Shandong University(No.2019SDRX-14,2019SDRX-15)the Fundamental Research Funds of Shandong UniversityQilu Young Scholar Foundation of Shandong University。
文摘Maintaining the stemness of the transplanted stem cell spheroids in an inflammatory microenvironment is challenging but important in regenerative medicine. Direct delivery of stem cells to repair periodontal defects may yield suboptimal effects due to the complexity of the periodontal inflammatory environment. Herein, stem cell spheroid is encapsulated by interfacial assembly of metal-phenolic network(MPN) nanofilm to form a stem cell microsphere capsule. Specifically, periodontal ligament stem cells(PDLSCs) spheroid was coated with FeⅢ/tannic acid coordination network to obtain spheroid@[FeⅢ-TA] microcapsules. The formed biodegradable MPN biointerface acted as a cytoprotective barrier and exhibited antioxidative, antibacterial and anti-inflammatory activities, effectively remodeling the inflammatory microenvironment and maintaining the stemness of PDLSCs. The stem cell microencapsulation proposed in this study can be applied to multiple stem cells with various functional metal ion/polyphenol coordination, providing a simple yet efficient delivery strategy for stem cell stemness maintenance in an inflammatory environment toward a better therapeutic outcome.
基金supported by the scientific research project of Baishanzu National Park(2021KFLY08)National Natural Science Foundation of China(31970447)+1 种基金Starting Fund for Doctoral Research of Lishui University(6004LMM01Z)Special Fund for Scientific Research of Postdoctoral Work Station Assessment in Zhejiang,China(2021,2023)。
基金partly supported by the National Natural Science Foundation of China(31970447)starting fund for doctoral research of Lishui University(6004LMM01Z)special fund for scientific research of postdoctoral research station assessment in Zhejiang Province,China(2021)。
基金partly supported by the National Natural Science Foundation of China(31970447)starting fund for doctoral research of Lishui University(6004LMM01Z)+1 种基金special fund for scientific research of postdoctoral work station assessment in Zhejiang ProvinceChina and GDAS Special Project of Science and Technology Development(2020GDASYL-20200102021,2020GDASYL-20200301003)。
