Technology of Extracting Kiwi Fruit Seed Oil with Ultrasonic-Assisted Enzyme and Response Surface Method

Taken kiwi fruit as raw material, this paper extracted kiwi fruit seed oil with ultrasonic-assisted enzyme, researched the influence of factors such as liquid-to-solid ratio, granularity, type of enzyme, ultrasonic power, treating time, enzymolysis temperature, enzymolysis time, pH and enzyme additive on oil extraction, and optimized the extracting technology of kiwi fruit seed oil with response surface method. The result shows that the best technical parameter is: material granularity: 60, liquid-to-solid ratio: 1:10 (g/mL), ultrasonic power: 400 W, treating time: 30 min, enzyme amount: 2.50%, pH: 9.2, enzymolysis temperature: 53°C, enzymolysis time: 2.80 h;and the extracting ratio under such condition is 92.57%.

Preparation of Microcapsules and Half Life of the Kiwi Fruit Seed Oil by Complex Coacervation

The experiment adopts complex coacervation to prepare microcapsules. Through the experimental comparison, soybean protein isolated-maltodextrin is determined as the wall material for the experimental preparation of the microcapsules of kiwi fruit seed oil. This paper researched the effects of wall material concentration, core wall ratio and other factors on complex coacervation of kiwi fruit seed oil microcapsules embedding rate, determining that the best wall material concentration is 1%, core wall ratio is 1:1, and the optimum pH ratio is 3.0, temperature is 40°C, and the optimum curing time is 6 hours. The experiment carried out half life research on the microcapsules prepared by the complex coacervation of kiwi fruit seed oil microcapsule. By calculation: the degradation rate constant of kiwi fruit seed oil microcapsules prepared by complex coacervation is 2.793. According to the regression equation it can calculate the half life of kiwi fruit seed oil microcapsules is 18.58 months, about a year and a half.

STRAP binds to and promotes the repair of N1-methyldeoxyadenosine in DNA

N1-methyladenosine(m1A)is an important RNA modification that functions in various biological processes by interacting with cellular proteins.However,the binding proteins of N1-methyldeoxyadenosine(1mdA)in DNA remain largely unknown.Herein,we employed a quantitative proteomics strategy to identify the potential binding proteins of 1mdA in human cells.Our results revealed that serine–threonine kinase receptor-associated protein(STRAP)can bind to 1mdA-carrying DNA.We further demonstrated that STRAP participates in alkylating agent-induced DNA damage response and can promote the repair of 1mdA embedded in DNA.Moreover,we investigated the effects of STRAP on 1mdA-induced perturbation in transcription using a shuttle vector-and next-generation sequencing-based assay,and found that STRAP is involved in the transcriptional bypass of 1mdA in human cells.Together,our study revealed STRAP as a novel 1mdA-binding protein in human cells and provided new insight into the biological implications of STRAP and 1mdA modification in human diseases.