Construction of Three-in-one Industry and Cultural Inheritance of Musical Instruments of Li People in Hainan from the Perspective of Rural Revitalization

From the perspective of industrial integration,how the musical instruments of Li people in Hainan can solve the current development problems such as lack of endogenous power and market enthusiasm under the strategy of rural revitalization to walk out a unique road of three-in-one industry construction was studied.

Molecular imaging:design mechanism and bioapplications

Molecular imaging is a non-invasive method to image and analyze the concentration and activity of functional biomolecules in cells or in vivo at molecular level,and plays an increasing role in deep understanding of biological processes,early and accurate diagnosis of diseases,and evaluation of treatment.Nowadays,numerous novel molecular imaging probes have been developed,involving every biomedical imaging modality,such as optical imaging,photoacoustic imaging,magnetic resonance imaging,single-photon-emission computed tomography,and positron emission tomography.In this review,we summarize the development of current state-of-the-art molecular imaging probes.We introduce the design strategies of molecular probes and detailed imaging modalities,and highlight the properties of probes and biomedical imaging applications in cells and in vivo,including disease diagnosis,drug tracking,and imaging-guided surgery.Then we discuss the perspectives and challenges in this emerging field.We expect this review could inspire more effective molecular imaging probes to be developed,achieving the goal towards clinical practices.

Photoactivatable Cell-Surface DNAzyme Sensor for Real-Time Monitoring of the Metal Ion-Induced AβAggregation

Precisely monitoring the distribution and local fluctuation of metal ions,which play vital roles in many physiological and pathological processes,contributes largely to the study of metal ion-induced pathogenesis.However,current reactive fluorescent sensors can hardly realize the monitoring of metal ions in situ due to the premature activation and aimless dispersion of the sensors,which hinder their applications in biological samples.Herein,we report a photoactivatable cell-surface DNAzyme sensor to monitor metal ions in the microenvironment of living cells or tissues with high spatial resolution and temporal control.The sensor can efficiently anchor to the cell membrane through amphipathic interaction,and the activity of the sensor can be restored after the irradiation by light,which avoids false positive signals from the reaction with metal cofactors during delivery.First,the properties of the sensor were investigated either in buffer or on amodel cell surface,inwhich the sensor responded to Zn2+with superior temporal and spatial resolution under light activation.Then,the sensor was applied tomonitor the dynamic changes ofmetal ions during the formation of Aβaggregates in the cellular microenvironment of brain cells and brain tissues.This strategy provides a potential tool to understand the roles ofmetal ions in the progression of diseases,such as Alzheimer’s disease.

Framework nucleic acid-based confined enzyme cascade for efficient synergistic cancer therapy in vivo

Artificial enzyme cascade systems with confinement effect are highly important in synthetic biology and biomedicine.Herein,a framework nucleic acid-based confined enzyme cascade(FNA-CEC)for synergistic cancer therapy in vivo was developed.The FNA-CEC consisted of glucose oxidase and horseradish peroxidase precisely assembled on an addressable DNA tetrahedron scaffold within few nanometers.Glucose oxidase(GOx)can trigger efficient glucose depletion for tumor starvation therapy,and increase the local concentration of H_(2)O_(2) in situ for enhanced downstream horseradish peroxidase(HRP)-activated prodrug therapy.Due to the spatial-confinement on DNA tetrahedron scaffold,the efficiency of intermediate metabolites transportation between the enzyme cascades was improved.Moreover,FNA-CEC was applied for efficient synergistic cancer therapy in vitro and in vivo.As a simple and efficient approach,the FNA-CEC is expected to expand the toolbox of technologies in synthetic biology and biomedicine.