Plant-based fermentations provide an untapped source for novel biotechnological applications.In this study,a probiotic named Lactobacillus fermentum 21828 was introduced to ferment Lentinus edodes.Polysaccharides were...Plant-based fermentations provide an untapped source for novel biotechnological applications.In this study,a probiotic named Lactobacillus fermentum 21828 was introduced to ferment Lentinus edodes.Polysaccharides were extracted from fermented and non-fermented L.edodes and purified via DEAE-52 and Sephadex G-100.The components designated F-LEP-2a and NF-LEP-2a were analyzed by FT-IR,HPGPC,HPAEC,SEM,GC-MS and NMR.The results revealed that probiotic fermentation increased the molecular weight from 1.16×10^(4) Da to 1.87×10^(4) Da and altered the proportions of glucose,galactose and mannose,in which glucose increased from 45.94%to 48.16%.Methylation analysis and NMR spectra indicated that F-LEP-2a and NF-LEP-2a had similar linkage patterns.Furthermore,their immunomodulatory activities were evaluated with immunosuppressive mice.NF-LEP and F-LEP improved immune organ indices,immunoglobulin(Ig G and Ig M)and cytokines concentrations;restored the antioxidation capacity of liver;and maintained the balance of gut microbiota.F-LEP displayed better moderating effects on the spleen index,immunoglobulin,cytokines and the diversity of gut microbiota than NF-LEP(200,400 mg/kg).Our study provides an efficient and environment-friendly way for the structural modification of polysaccharides,which helps to enhance their biological activity and promote their wide application in food,medicine and other fields.展开更多
This paper used Active Radio Frequency Identification （Active RFID） technology to identify in which rooms fathers and their chitd tend to stay together and talk, and in which rooms they stay separately in seven one-...This paper used Active Radio Frequency Identification （Active RFID） technology to identify in which rooms fathers and their chitd tend to stay together and talk, and in which rooms they stay separately in seven one-child families living in Chinese urban apartment houses. The father was found to stay together with the child 0.5%-25% of the time when both father and chitd stayed at home. The use of the living room as the place in which the child stays with the father and tatks was found to be highest （five out of seven families）, fotlowed by the dining room and the child＇s room. In over half of the cases when the child stays with,the father in the living room or dining room and either of them tatk, the chitd spoke over 1.6 times more than the father. However, in the child＇s room, the child always spoke tess than the father, and the duration of the child＇s speech was less than 70% of that of the father. Findings showed that the instances in which child and father stay in different rooms fell into two groups. First, five of the seven subject fathers tended to stay in the living room, whereas the children stayed either in their room or in their parents＇ room to use the PC. Second, two fathers stayed in the studio or dining room to work, while their chitdren stayed in the living room or their own rooms. For both groups, the duration展开更多
基金supported by grants from the National Key R&D Program of China(2019YFC1606701)。
文摘Plant-based fermentations provide an untapped source for novel biotechnological applications.In this study,a probiotic named Lactobacillus fermentum 21828 was introduced to ferment Lentinus edodes.Polysaccharides were extracted from fermented and non-fermented L.edodes and purified via DEAE-52 and Sephadex G-100.The components designated F-LEP-2a and NF-LEP-2a were analyzed by FT-IR,HPGPC,HPAEC,SEM,GC-MS and NMR.The results revealed that probiotic fermentation increased the molecular weight from 1.16×10^(4) Da to 1.87×10^(4) Da and altered the proportions of glucose,galactose and mannose,in which glucose increased from 45.94%to 48.16%.Methylation analysis and NMR spectra indicated that F-LEP-2a and NF-LEP-2a had similar linkage patterns.Furthermore,their immunomodulatory activities were evaluated with immunosuppressive mice.NF-LEP and F-LEP improved immune organ indices,immunoglobulin(Ig G and Ig M)and cytokines concentrations;restored the antioxidation capacity of liver;and maintained the balance of gut microbiota.F-LEP displayed better moderating effects on the spleen index,immunoglobulin,cytokines and the diversity of gut microbiota than NF-LEP(200,400 mg/kg).Our study provides an efficient and environment-friendly way for the structural modification of polysaccharides,which helps to enhance their biological activity and promote their wide application in food,medicine and other fields.
文摘This paper used Active Radio Frequency Identification （Active RFID） technology to identify in which rooms fathers and their chitd tend to stay together and talk, and in which rooms they stay separately in seven one-child families living in Chinese urban apartment houses. The father was found to stay together with the child 0.5%-25% of the time when both father and chitd stayed at home. The use of the living room as the place in which the child stays with the father and tatks was found to be highest （five out of seven families）, fotlowed by the dining room and the child＇s room. In over half of the cases when the child stays with,the father in the living room or dining room and either of them tatk, the chitd spoke over 1.6 times more than the father. However, in the child＇s room, the child always spoke tess than the father, and the duration of the child＇s speech was less than 70% of that of the father. Findings showed that the instances in which child and father stay in different rooms fell into two groups. First, five of the seven subject fathers tended to stay in the living room, whereas the children stayed either in their room or in their parents＇ room to use the PC. Second, two fathers stayed in the studio or dining room to work, while their chitdren stayed in the living room or their own rooms. For both groups, the duration
