AIM: To analyze the clinical efficacy of intravitreal injection of ranibizumab(IVR) on diabetic macular edema(DME) with multifocal electroretinography(mf ERG) and optical coherence tomography(OCT).METHODS: A total of ...AIM: To analyze the clinical efficacy of intravitreal injection of ranibizumab(IVR) on diabetic macular edema(DME) with multifocal electroretinography(mf ERG) and optical coherence tomography(OCT).METHODS: A total of 41 patients(41 eyes) with DME were treated with IVR. The best corrected visual acuity(BCVA), mf ERG results, and OCT were analyzed to compare to the baselines at 1 wk, 1, and 3 mo after operation.RESULTS: The BCVA was significantly improved in all eyes at each time point(P<0.001). The macular area leakage and edema were reduced 1 wk and 1 mo after IVR, and the central fovea thickness(CFT) was significantly reduced compared to baseline(P<0.001). The mf ERG, twodimensional and three-dimensional images all showed that the macular fovea(1 ring) response density decreased, and the fovea and macular area spikes significantly decreased or disappeared. The amplitude density of the P1 wave was increased, and the latency of the P1 wave was shortened than preoperation(P<0.001). At 1 wk and 1 mo after the operation, there was a negative correlation between the amplitude density of P1 waves and CFT.CONCLUSION: OCT and mf ERG fully demonstrate the importance of IVR for DME patients from the macular morphology and function, especially the significance of mf ERG in this disease.展开更多
Polysaccharides from genus Ganoderma have prominent anti-aging effect,but their mechanisms are incompletely clarified.In our previous experiments,Ganoderma atrum polysaccharide(PSG)was exhibited to significantly allev...Polysaccharides from genus Ganoderma have prominent anti-aging effect,but their mechanisms are incompletely clarified.In our previous experiments,Ganoderma atrum polysaccharide(PSG)was exhibited to significantly alleviate senescence and DNA damage of A375 cells.To investigate its underlying mechanism,we conducted RNA-Seq analysis and found that PSG upregulated autophagy and mitochondria pathways compared to the model group.Further experiments showed that PSG relieved mitochondrial dysfunction via inhibiting the accumulation of reactive oxygen species(ROS)and loss of the mitochondrial membrane potential(MMP).Meanwhile,PSG activated autophagy and enhanced lysosomal function through transcription factor EB(TFEB).Interestingly,the pretreatment of autophagy inhibitors bafilomycin A1(Baf A1)reversed the ROS decline induced by PSG,which subsequently increased cellular senescence of A375.In addition,PSG had a similar anti-aging effect on normal fibroblast WI-38.Taken together,PSG might reduce ROS levels through activating autophagy,which in turn suppressed mitochondrial dysfunction and cellular senescence.展开更多
文摘AIM: To analyze the clinical efficacy of intravitreal injection of ranibizumab(IVR) on diabetic macular edema(DME) with multifocal electroretinography(mf ERG) and optical coherence tomography(OCT).METHODS: A total of 41 patients(41 eyes) with DME were treated with IVR. The best corrected visual acuity(BCVA), mf ERG results, and OCT were analyzed to compare to the baselines at 1 wk, 1, and 3 mo after operation.RESULTS: The BCVA was significantly improved in all eyes at each time point(P<0.001). The macular area leakage and edema were reduced 1 wk and 1 mo after IVR, and the central fovea thickness(CFT) was significantly reduced compared to baseline(P<0.001). The mf ERG, twodimensional and three-dimensional images all showed that the macular fovea(1 ring) response density decreased, and the fovea and macular area spikes significantly decreased or disappeared. The amplitude density of the P1 wave was increased, and the latency of the P1 wave was shortened than preoperation(P<0.001). At 1 wk and 1 mo after the operation, there was a negative correlation between the amplitude density of P1 waves and CFT.CONCLUSION: OCT and mf ERG fully demonstrate the importance of IVR for DME patients from the macular morphology and function, especially the significance of mf ERG in this disease.
基金This work was supported by the National Science Fund for Distinguished Young Scholars of China(31825020)the Technology Innovation Guidance Program of Jiangxi Province(20203AEI91008)Key laboratory of Bioactive Polysaccharides of Jiangxi Province(20212BCD42016).
文摘Polysaccharides from genus Ganoderma have prominent anti-aging effect,but their mechanisms are incompletely clarified.In our previous experiments,Ganoderma atrum polysaccharide(PSG)was exhibited to significantly alleviate senescence and DNA damage of A375 cells.To investigate its underlying mechanism,we conducted RNA-Seq analysis and found that PSG upregulated autophagy and mitochondria pathways compared to the model group.Further experiments showed that PSG relieved mitochondrial dysfunction via inhibiting the accumulation of reactive oxygen species(ROS)and loss of the mitochondrial membrane potential(MMP).Meanwhile,PSG activated autophagy and enhanced lysosomal function through transcription factor EB(TFEB).Interestingly,the pretreatment of autophagy inhibitors bafilomycin A1(Baf A1)reversed the ROS decline induced by PSG,which subsequently increased cellular senescence of A375.In addition,PSG had a similar anti-aging effect on normal fibroblast WI-38.Taken together,PSG might reduce ROS levels through activating autophagy,which in turn suppressed mitochondrial dysfunction and cellular senescence.
