Due to the wide application of rare earth elements,lanthanum(La) is gradually accumulated in our living environment.Lanthanum can enter the body through a variety of routes,which has a series of effects on various sys...Due to the wide application of rare earth elements,lanthanum(La) is gradually accumulated in our living environment.Lanthanum can enter the body through a variety of routes,which has a series of effects on various systems.As a consequence,the safety of lanthanum deserves our attention.This study aims to compare the toxicity of lanthanum oxide nanoparticles(La_(2)O_(3) NPs) and lanthanum chloride(LaCl_(3)) and enrich the toxicity evaluation of lanthanum.Therefore,a 30 d intragastal experiment was conducted.C57BL/6j mice were given by La_(2)O_(3) NPs solution and LaCl_(3) solution respectively at doses of 0,30,60 and120 mg/kg BW(lanthanum content).The results show that,compared with the control group,both La_(2)O_(3)NPs and LaCl_(3) can reduce the body weight of female mice in the high-dose group,but for male mice,the high-dose La_(2)O_(3) NPs solution can increase the body weight,while LaCl_(3) has the opposite effect.The coefficients of liver,kidney,heart,lung,uterine and ovaries increase first and then decrease with the exposure dose,but testes and epididymides coefficient keep increasing with the exposure of LaCl_(3) and La_(2)O_(3) NPs.La_(2)O_(3) NPs and LaCl_(3) can reduce the concentration of triglycerides(TG) and increase the level of low density lipoprotein(LDL),but the effect of La_(2)O_(3) NPs is more obvious.La_(2)O_(3) NPs and LaCl_(3) can reduce the concentration of malondialdehyde(MDA),increase the total antioxidant capacity(T-AOC)and enhance the activity of catalase(CAT),but LaCl_(3) has more obvious protective effects on oxidative stress.La_(2)O_(3) NPs and LaCl_(3) have potential risks to liver,and the toxicity of La_(2)O_(3) NPs might be higher than that of LaCl_(3).展开更多
Tyrosine Phosphorylation(pTyr)is a critical and ubiquitous regulation mechanism in biology that plays a central role in controlling intracellular signaling networks.Precise recognition and specific detection of pTyr p...Tyrosine Phosphorylation(pTyr)is a critical and ubiquitous regulation mechanism in biology that plays a central role in controlling intracellular signaling networks.Precise recognition and specific detection of pTyr peptides have been of great importance for both discoveries of disease biomarkers and screening of therapeutic drugs,especially cancers.Here we report a label-free,versatile,realtime,and high-throughput detection strategy for phosphopeptide(PP)based on reversible configuration freeze of a unique hemicyanine-labeled 2-(2′-hydroxyphenyl)-4-methyloxazole(H-HPMO).By taking advantage of the“OFF–ON”transition of fluorescence,H-HPMO–Cu^(2+)complex displays a highly sensitive and selective response to PPs with modified sites on serine,threonine,and tyrosine.Specific recognition of Tyr PPs is achieved by performing a simple logic gate operation and introducing Ca^(2+)interference as an input.This PP detection approach is universal for various peptide sequences and displays high potential in large-scale kinase inhibitor screening,which will promote the development of targeted anticancer drugs.展开更多
Nature has selected,produced,and evolved numerous molecular architectural motifs over billions of years for particular bio-functions.During this process,molecular self-assembly plays a critically important role.Inspir...Nature has selected,produced,and evolved numerous molecular architectural motifs over billions of years for particular bio-functions.During this process,molecular self-assembly plays a critically important role.Inspired by these delicate assemblies,scientists devote themselves to developing various sophisticated complexes assembled by diverse and numerous structural motifs.By far,most of the examples focus on single-molecule self-assembly or co-assembly between two molecules,seldom works report the co-assembly among three building blocks due to the substantially increased complexity and decreased controllability.Here we report a novel three-component co-assembly among L-glutathione(L-GSH),AgNO_(3),and an aggregation-induced emission luminogen(AIEgen),namely triphenylamine-pyridinium(abbreviated to TPA-Py).TPA-Py,L-GSH,and Ag^(+)co-assemble into numerous long and ordered nanowires with left-handed helices,which show remarkable AIE properties that can be observed by naked eyes.This effect is only detected in this system,the replacement of TPA-Py with other AIEgens,L-GSH with other cysteine derivatives or anions,Ag^(+)with other metal ions,could not lead to the unique AIE effect,which endows this system with high specificity and controllability.This work provides new insight into the design of biomolecule recognition systems and discloses that ternary co-assembly could become a facile route to build sophisticated nanobiomaterials.展开更多
Volatile organic compounds(VOCs)with high toxicity and carcinogenicity are emitted from kinds of industries,which endanger human health and the environment.Adsorption is a promising method for the treatment of VOCs du...Volatile organic compounds(VOCs)with high toxicity and carcinogenicity are emitted from kinds of industries,which endanger human health and the environment.Adsorption is a promising method for the treatment of VOCs due to its low cost and high efficiency.In recent years,activated carbons,zeolites,and mesoporous materials are widely used to remove VOCs because of their high specific surface area and abundant porosity.However,the hydrophilic nature and low desorption rate of those materials limit their commercial application.Furthermore,the adsorption capacities of VOCs still need to be improved.Porous organic polymers(POPs)with extremely high porosity,structural diversity,and hydrophobic have been considered as one of the most promising candidates for VOCs adsorption.This review generalized the superiority of POPs for VOCs adsorption compared to other porous materials and summarized the studies of VOCs adsorption on different types of POPs.Moreover,the mechanism of competitive adsorption between water and VOCs on the POPs was discussed.Finally,a concise outlook for utilizing POPs for VOCs adsorption was discussed,noting areas in which further work is needed to develop the next-generation POPs for practical applications.展开更多
Multifunctional nanocarriers with multilayer core-shell architecture were prepared by coating superparamagnetic Fe3O4 nanoparticles with diblock copolymer folate-poly(ethylene glycol)-b-poly(glycerol monomethacrylate)...Multifunctional nanocarriers with multilayer core-shell architecture were prepared by coating superparamagnetic Fe3O4 nanoparticles with diblock copolymer folate-poly(ethylene glycol)-b-poly(glycerol monomethacrylate)(FA-PEG-bPGMA), and triblock copolymer methoxy poly(ethylene glycol)-b-poly(2-(dimethylamino) ethyl methacrylate)-bpoly(glycerol monomethacrylate)(MPEG-b-PDMA-b-PGMA). The PGMA segment was attached to the surfaces of Fe3O4 nanoparticles, and the outer PEG shell imparted biocompatibility. In addition, folate was conjugated onto the surfaces of the nanocarriers. Cisplatin was then loaded into the nanocarrier by coordination between the Pt atom in cisplatin and the amine groups in the inner shell of the multilayer architecture. The loaded cisplatin showed pH-responsive release: slower release at pH 7.4(i.e. mimicking the blood environment) and faster release at more acidic pH(i.e. mimicking endosome/lysosome conditions). All of the cisplatin-loaded nanoparticles showed concentration-dependent cytotoxicity in HeLa cells. However, the folate-conjugated cisplatin-loaded carriers exhibited higher cytotoxicity in HeLa cells than non-folate conjugated cisplatin-loaded carriers.展开更多
An experimental study to investigate various features of subsurface vortices and their evolutions inside a closed pump intake under different pressure conditions at free surface,and to analyze the influence of a pipe ...An experimental study to investigate various features of subsurface vortices and their evolutions inside a closed pump intake under different pressure conditions at free surface,and to analyze the influence of a pipe bell with different mean axial velocities at the same water level on flow patterns is presented.For all cases,three different flow regimes are closely observed:various side-wall attached,back-wall attached and submerged vortices structures,and their evolutions are subject to the free-surface pressure for a critical flow rate,the number and strength of all these vortex rise as the absolute value of the pressure increases for a higher flow rate,for a lower flow rate,whether the side-wall vortices occur or not is related closely to the pressure magnitude,and there are no vortices for lower absolute pressure values,the number and the strength of the side-wall vortices increase as the absolute pressure values increase.Besides,based on statistical analyses,the side-wall vortex probability on the right side along the stream-wise direction is much higher than that on the opposite side for all operation cases,particularly single vortex probability,and the back-wall vortex probabilities are quite higher with values of more than 90%,meanwhile,the multi-vortices probability is much higher than that of the single-vortex.The captured vortices have been qualitatively classified into different“vortex zones”:no vortex zones and vortex zones including transition,single-and multi-vortex.The Reynolds number and the absolute value pressure are the most important parameters which influence the existence and the number of these vortices,and the increase of the two parameters reduces the no vortex transition zone and causes bigger difference in the back-wall and side-wall vortex zones.展开更多
基金Project supported by the National Natural Science Foundation of China(81903364)the National Natural Science Foundation of Jiangxi Province(20202BABL206123)the Postgraduate Innovation Special Fund Project of Jiangxi Province(YC2021-S795)。
文摘Due to the wide application of rare earth elements,lanthanum(La) is gradually accumulated in our living environment.Lanthanum can enter the body through a variety of routes,which has a series of effects on various systems.As a consequence,the safety of lanthanum deserves our attention.This study aims to compare the toxicity of lanthanum oxide nanoparticles(La_(2)O_(3) NPs) and lanthanum chloride(LaCl_(3)) and enrich the toxicity evaluation of lanthanum.Therefore,a 30 d intragastal experiment was conducted.C57BL/6j mice were given by La_(2)O_(3) NPs solution and LaCl_(3) solution respectively at doses of 0,30,60 and120 mg/kg BW(lanthanum content).The results show that,compared with the control group,both La_(2)O_(3)NPs and LaCl_(3) can reduce the body weight of female mice in the high-dose group,but for male mice,the high-dose La_(2)O_(3) NPs solution can increase the body weight,while LaCl_(3) has the opposite effect.The coefficients of liver,kidney,heart,lung,uterine and ovaries increase first and then decrease with the exposure dose,but testes and epididymides coefficient keep increasing with the exposure of LaCl_(3) and La_(2)O_(3) NPs.La_(2)O_(3) NPs and LaCl_(3) can reduce the concentration of triglycerides(TG) and increase the level of low density lipoprotein(LDL),but the effect of La_(2)O_(3) NPs is more obvious.La_(2)O_(3) NPs and LaCl_(3) can reduce the concentration of malondialdehyde(MDA),increase the total antioxidant capacity(T-AOC)and enhance the activity of catalase(CAT),but LaCl_(3) has more obvious protective effects on oxidative stress.La_(2)O_(3) NPs and LaCl_(3) have potential risks to liver,and the toxicity of La_(2)O_(3) NPs might be higher than that of LaCl_(3).
基金This work was financially supported by the National Natural Science Foundation of China(grant nos.21775116,21922411,and 21934005)Dalian Institute of Chemical Physics(DICP)Innovation Funding(grant nos.RC201801 and I202008)the LiaoNing Revitalization Talents Program(grant no.XLYC1802109).
文摘Tyrosine Phosphorylation(pTyr)is a critical and ubiquitous regulation mechanism in biology that plays a central role in controlling intracellular signaling networks.Precise recognition and specific detection of pTyr peptides have been of great importance for both discoveries of disease biomarkers and screening of therapeutic drugs,especially cancers.Here we report a label-free,versatile,realtime,and high-throughput detection strategy for phosphopeptide(PP)based on reversible configuration freeze of a unique hemicyanine-labeled 2-(2′-hydroxyphenyl)-4-methyloxazole(H-HPMO).By taking advantage of the“OFF–ON”transition of fluorescence,H-HPMO–Cu^(2+)complex displays a highly sensitive and selective response to PPs with modified sites on serine,threonine,and tyrosine.Specific recognition of Tyr PPs is achieved by performing a simple logic gate operation and introducing Ca^(2+)interference as an input.This PP detection approach is universal for various peptide sequences and displays high potential in large-scale kinase inhibitor screening,which will promote the development of targeted anticancer drugs.
基金National Natural Science Foundation of China,Grant/Award Numbers:21501055,21922411,22174138DICP Innovation Funding,Grant/Award Numbers:RC201801,I202008Dalian Outstanding Young Scientific Talent,Grant/Award Number:2020RJ01。
文摘Nature has selected,produced,and evolved numerous molecular architectural motifs over billions of years for particular bio-functions.During this process,molecular self-assembly plays a critically important role.Inspired by these delicate assemblies,scientists devote themselves to developing various sophisticated complexes assembled by diverse and numerous structural motifs.By far,most of the examples focus on single-molecule self-assembly or co-assembly between two molecules,seldom works report the co-assembly among three building blocks due to the substantially increased complexity and decreased controllability.Here we report a novel three-component co-assembly among L-glutathione(L-GSH),AgNO_(3),and an aggregation-induced emission luminogen(AIEgen),namely triphenylamine-pyridinium(abbreviated to TPA-Py).TPA-Py,L-GSH,and Ag^(+)co-assemble into numerous long and ordered nanowires with left-handed helices,which show remarkable AIE properties that can be observed by naked eyes.This effect is only detected in this system,the replacement of TPA-Py with other AIEgens,L-GSH with other cysteine derivatives or anions,Ag^(+)with other metal ions,could not lead to the unique AIE effect,which endows this system with high specificity and controllability.This work provides new insight into the design of biomolecule recognition systems and discloses that ternary co-assembly could become a facile route to build sophisticated nanobiomaterials.
基金supported by the National Key R&D Pro-gram of China(Nos.2019YFC1904100,2019YFC1904102,2019YFC1903902,and 2016YFC0205300)the National En-gineering Laboratory for Mobile Source Emission Control Technology of China(No.NELMS2017A03)+3 种基金the Natural Na-tional Science Foundation of China(Nos.21503144,21690083)Tianjin Research Program of Ecological Environmental Treat-ment(Nos.18ZXSZSF00210,18ZXSZSF00060)the Tianjin Research Program of Application Foundation and Advanced Technique(No.16JCQNJC05400)Major Science and Tech-nology Project for Ecological Environment Management in Tianjin(No.18ZXSZSF00210)。
文摘Volatile organic compounds(VOCs)with high toxicity and carcinogenicity are emitted from kinds of industries,which endanger human health and the environment.Adsorption is a promising method for the treatment of VOCs due to its low cost and high efficiency.In recent years,activated carbons,zeolites,and mesoporous materials are widely used to remove VOCs because of their high specific surface area and abundant porosity.However,the hydrophilic nature and low desorption rate of those materials limit their commercial application.Furthermore,the adsorption capacities of VOCs still need to be improved.Porous organic polymers(POPs)with extremely high porosity,structural diversity,and hydrophobic have been considered as one of the most promising candidates for VOCs adsorption.This review generalized the superiority of POPs for VOCs adsorption compared to other porous materials and summarized the studies of VOCs adsorption on different types of POPs.Moreover,the mechanism of competitive adsorption between water and VOCs on the POPs was discussed.Finally,a concise outlook for utilizing POPs for VOCs adsorption was discussed,noting areas in which further work is needed to develop the next-generation POPs for practical applications.
基金financially supported by the National Natural Science Foundation of China(Nos.51373080,81001417 and 20974052)PCSIRT(No.IRT1257)+1 种基金the National Key Technologies R&D Program for New Drugs of China(No.2009ZX09301-002)the Natural Science Foundation of Tianjin Municipality(No.09JCZDJC22900)
文摘Multifunctional nanocarriers with multilayer core-shell architecture were prepared by coating superparamagnetic Fe3O4 nanoparticles with diblock copolymer folate-poly(ethylene glycol)-b-poly(glycerol monomethacrylate)(FA-PEG-bPGMA), and triblock copolymer methoxy poly(ethylene glycol)-b-poly(2-(dimethylamino) ethyl methacrylate)-bpoly(glycerol monomethacrylate)(MPEG-b-PDMA-b-PGMA). The PGMA segment was attached to the surfaces of Fe3O4 nanoparticles, and the outer PEG shell imparted biocompatibility. In addition, folate was conjugated onto the surfaces of the nanocarriers. Cisplatin was then loaded into the nanocarrier by coordination between the Pt atom in cisplatin and the amine groups in the inner shell of the multilayer architecture. The loaded cisplatin showed pH-responsive release: slower release at pH 7.4(i.e. mimicking the blood environment) and faster release at more acidic pH(i.e. mimicking endosome/lysosome conditions). All of the cisplatin-loaded nanoparticles showed concentration-dependent cytotoxicity in HeLa cells. However, the folate-conjugated cisplatin-loaded carriers exhibited higher cytotoxicity in HeLa cells than non-folate conjugated cisplatin-loaded carriers.
基金supported by the National Natural Science Foundation of China(Grant Nos.51479196,51779257)the National Natural Science Foundation for the Youth of China(Grant No.51909132).
文摘An experimental study to investigate various features of subsurface vortices and their evolutions inside a closed pump intake under different pressure conditions at free surface,and to analyze the influence of a pipe bell with different mean axial velocities at the same water level on flow patterns is presented.For all cases,three different flow regimes are closely observed:various side-wall attached,back-wall attached and submerged vortices structures,and their evolutions are subject to the free-surface pressure for a critical flow rate,the number and strength of all these vortex rise as the absolute value of the pressure increases for a higher flow rate,for a lower flow rate,whether the side-wall vortices occur or not is related closely to the pressure magnitude,and there are no vortices for lower absolute pressure values,the number and the strength of the side-wall vortices increase as the absolute pressure values increase.Besides,based on statistical analyses,the side-wall vortex probability on the right side along the stream-wise direction is much higher than that on the opposite side for all operation cases,particularly single vortex probability,and the back-wall vortex probabilities are quite higher with values of more than 90%,meanwhile,the multi-vortices probability is much higher than that of the single-vortex.The captured vortices have been qualitatively classified into different“vortex zones”:no vortex zones and vortex zones including transition,single-and multi-vortex.The Reynolds number and the absolute value pressure are the most important parameters which influence the existence and the number of these vortices,and the increase of the two parameters reduces the no vortex transition zone and causes bigger difference in the back-wall and side-wall vortex zones.
