DMRT1(Double sex and Mab-3 related transcription factor 1)是鸡性腺分化和发育的关键候选基因,其详细的功能和作用途径还未被阐明。为了解明DMRT1在鸡性腺分化和发育过程中的功能,研究首次利用在线sg RNA平台设计4条鸡DMRT1 sg RNA...DMRT1(Double sex and Mab-3 related transcription factor 1)是鸡性腺分化和发育的关键候选基因,其详细的功能和作用途径还未被阐明。为了解明DMRT1在鸡性腺分化和发育过程中的功能,研究首次利用在线sg RNA平台设计4条鸡DMRT1 sg RNA序列,构建了4个sg RNA表达载体:Guide-1、Guide-2、Guide-3和Guide-4。转染鸡DF-1成纤维细胞后,利用T7E1内切酶的方法检测4个表达载体的打靶效率。结果显示,成功构建了4条正确的鸡DMRT1 sg RNA表达载体,并且都能高效地在DF-1细胞中表达,其中Guide-4表达载体能有效地打靶DMRT1基因,打靶效率为24.82%。研究结果为进一步在鸡胚敲除DMRT1基因,研究其功能奠定了基础。展开更多
Salicylic acid(SA) or 2-hydroxybenzoic acid,better known as the active ingredient in aspirin,is a phenolic plant hormone that plays an essential role in plant defense against biotrophic and semi-biotrophic pathogens(F...Salicylic acid(SA) or 2-hydroxybenzoic acid,better known as the active ingredient in aspirin,is a phenolic plant hormone that plays an essential role in plant defense against biotrophic and semi-biotrophic pathogens(Fu and Dong,2013).展开更多
文摘DMRT1(Double sex and Mab-3 related transcription factor 1)是鸡性腺分化和发育的关键候选基因,其详细的功能和作用途径还未被阐明。为了解明DMRT1在鸡性腺分化和发育过程中的功能,研究首次利用在线sg RNA平台设计4条鸡DMRT1 sg RNA序列,构建了4个sg RNA表达载体:Guide-1、Guide-2、Guide-3和Guide-4。转染鸡DF-1成纤维细胞后,利用T7E1内切酶的方法检测4个表达载体的打靶效率。结果显示,成功构建了4条正确的鸡DMRT1 sg RNA表达载体,并且都能高效地在DF-1细胞中表达,其中Guide-4表达载体能有效地打靶DMRT1基因,打靶效率为24.82%。研究结果为进一步在鸡胚敲除DMRT1基因,研究其功能奠定了基础。
基金The work in the Fu lab is supported by the National Science Foundation(EAGER grant 1464527 and grant IOS-1758994 to Z.Q.F.).
文摘Salicylic acid(SA) or 2-hydroxybenzoic acid,better known as the active ingredient in aspirin,is a phenolic plant hormone that plays an essential role in plant defense against biotrophic and semi-biotrophic pathogens(Fu and Dong,2013).
