In this formulation study,biocompatible non steroidal anti-inflammatory(NSAIDs)-loaded nanoparticles were designed as models to be further integrated in a prosthesis surface functionalization.A modified spontaneous em...In this formulation study,biocompatible non steroidal anti-inflammatory(NSAIDs)-loaded nanoparticles were designed as models to be further integrated in a prosthesis surface functionalization.A modified spontaneous emulsion-solvent diffusion methodology was used to produce drug-loaded PLGA nanoparticles without any purification or solvent evaporation requirements.Formulation parameters,such as lactide/glycolide ratio,polymer concentration,solvent/non solvent ratio and non solvent phase,as well as the non ionic tensioactive P188 co-precipitation composition were systematically explored.The optimized formulation(mean size:145 nm,surface charge:-13 m V) was employed to encapsulate various amounts of NSAIDs in a simple and scalable manner.The drug release was characterized in vitro by a complete release for 48 h.These results encourage upcoming preliminary steps for in vivo experiments of prosthesis surface functionalization.展开更多
Deciphering breast cancer treatment resistance remains hindered by the lack of models that can successfully capture the four-dimensional dynamics of the tumor microenvironment.Here,we show that microextrusion bioprint...Deciphering breast cancer treatment resistance remains hindered by the lack of models that can successfully capture the four-dimensional dynamics of the tumor microenvironment.Here,we show that microextrusion bioprinting can reproducibly generate distinct cancer and stromal compartments integrating cells relevant to human pathology.Our findings unveil the functional maturation of this millimeter-sized model,showcasing the development of a hypoxic cancer core and an increased surface proliferation.Maturation was also driven by the presence of cancer-associated fibroblasts(CAF)that induced elevated microvascular-like structures complexity.Such modulation was concomitant to extracellular matrix remodeling,with high levels of collagen and matricellular proteins deposition by CAF,simultaneously increasing tumor stiffness and recapitulating breast cancer fibrotic development.Importantly,our bioprinted model faithfully reproduced response to treatment,further modulated by CAF.Notably,CAF played a protective role for cancer cells against radiotherapy,facilitating increased paracrine communications.This model holds promise as a platform to decipher interactions within the microenvironment and evaluate stroma-targeted drugs in a context relevant to human pathology.展开更多
基金financially supported by the European Erasmus program
文摘In this formulation study,biocompatible non steroidal anti-inflammatory(NSAIDs)-loaded nanoparticles were designed as models to be further integrated in a prosthesis surface functionalization.A modified spontaneous emulsion-solvent diffusion methodology was used to produce drug-loaded PLGA nanoparticles without any purification or solvent evaporation requirements.Formulation parameters,such as lactide/glycolide ratio,polymer concentration,solvent/non solvent ratio and non solvent phase,as well as the non ionic tensioactive P188 co-precipitation composition were systematically explored.The optimized formulation(mean size:145 nm,surface charge:-13 m V) was employed to encapsulate various amounts of NSAIDs in a simple and scalable manner.The drug release was characterized in vitro by a complete release for 48 h.These results encourage upcoming preliminary steps for in vivo experiments of prosthesis surface functionalization.
基金the financial support of INSERM(France)and"Fondation ARC pour la recherche sur le cancer".
文摘Deciphering breast cancer treatment resistance remains hindered by the lack of models that can successfully capture the four-dimensional dynamics of the tumor microenvironment.Here,we show that microextrusion bioprinting can reproducibly generate distinct cancer and stromal compartments integrating cells relevant to human pathology.Our findings unveil the functional maturation of this millimeter-sized model,showcasing the development of a hypoxic cancer core and an increased surface proliferation.Maturation was also driven by the presence of cancer-associated fibroblasts(CAF)that induced elevated microvascular-like structures complexity.Such modulation was concomitant to extracellular matrix remodeling,with high levels of collagen and matricellular proteins deposition by CAF,simultaneously increasing tumor stiffness and recapitulating breast cancer fibrotic development.Importantly,our bioprinted model faithfully reproduced response to treatment,further modulated by CAF.Notably,CAF played a protective role for cancer cells against radiotherapy,facilitating increased paracrine communications.This model holds promise as a platform to decipher interactions within the microenvironment and evaluate stroma-targeted drugs in a context relevant to human pathology.
