<strong>Aim:</strong> Diabetes Mellitus is a global public health challenge with major and potentially devastating complications, and concomitant complications include retinopathy and neuropathy due to hyp...<strong>Aim:</strong> Diabetes Mellitus is a global public health challenge with major and potentially devastating complications, and concomitant complications include retinopathy and neuropathy due to hypoxia and microvascular dysfunction. In this study, we investigated the effect of hyperbaric oxygen therapy as a method to transiently improve tissue oxygenation on diabetic retinopathy and neuropathy in a streptozotocin induced type-1 diabetic rat model (Wistar). <strong>Methods:</strong> Streptozotocin induced type-1 diabetic rats received 10 sessions of 2-h hyperbaric oxygen exposures (pO<sub>2</sub> = 309 kPa) over 2 weeks. Animals were exposed to light stimuli to produce light evoked potentials to estimate the effect of oxygen treatment on diabetic retinopathy. Sciatic nerves were exposed and stimulated to produce muscle evoked potential, which were recorded in the muscles of the foot and subsequently used to evaluate the effect of oxygen treatment on diabetic neuropathy. <strong>Results:</strong> We found significantly shorter light evoked potential latency and increased amplitude in hyperbaric oxygen treated animals. No change was found in nerve conduction. <strong>Conclusions:</strong> This study showed that hyperbaric oxygen therapy is a potentially effective treatment for diabetic retinopathy, improving both latency and amplitude of light evoked potentials.展开更多
Background: The aim of this study was to evaluate the feasibility of a dualbolus protocol, where a first bolus of an intravascular tracer is used to measure perfusion, followed by a second bolus of a freely filtered g...Background: The aim of this study was to evaluate the feasibility of a dualbolus protocol, where a first bolus of an intravascular tracer is used to measure perfusion, followed by a second bolus of a freely filtered gadolinium-containing agent to measure filtration capacity. Methods: The study was conducted in mice subjected to complete unilateral ureteral obstruction (UUO), and sham operated mice were used as controls. Dynamic contrast- en-hanced MRI was performed 2 days after surgery. Results and discussions: Mean signal-time curves of the renal cortex, renal medulla and abdominal aorta were used to calculate the relative renal blood flow (rRBF), relative renal blood volume (rRBV), mean transit time (MTT) and the glomerular transfer rate Ktrans. We demonstrated that kidneys suffering from two days of UUO showed a decrease in cortical as well as medullary rRBF compared to kidneys from sham-operated mice. Further, we found no changes in rRBV and MTT among groups, neither in the cortex nor in the medulla. The renal functional parameter Ktrans showed a tendency (but statistically insignificant) to be reduced in the ob-structed kidney compared to the sham-operated mice. Conclusions: We showed our first experiences with the consecutive use of intra- and extra-vascularly distributed agents in a renal-diseased mouse model, allowing analysis of both functional haemo- dyamics and filtration capacity in kidneys.展开更多
文摘<strong>Aim:</strong> Diabetes Mellitus is a global public health challenge with major and potentially devastating complications, and concomitant complications include retinopathy and neuropathy due to hypoxia and microvascular dysfunction. In this study, we investigated the effect of hyperbaric oxygen therapy as a method to transiently improve tissue oxygenation on diabetic retinopathy and neuropathy in a streptozotocin induced type-1 diabetic rat model (Wistar). <strong>Methods:</strong> Streptozotocin induced type-1 diabetic rats received 10 sessions of 2-h hyperbaric oxygen exposures (pO<sub>2</sub> = 309 kPa) over 2 weeks. Animals were exposed to light stimuli to produce light evoked potentials to estimate the effect of oxygen treatment on diabetic retinopathy. Sciatic nerves were exposed and stimulated to produce muscle evoked potential, which were recorded in the muscles of the foot and subsequently used to evaluate the effect of oxygen treatment on diabetic neuropathy. <strong>Results:</strong> We found significantly shorter light evoked potential latency and increased amplitude in hyperbaric oxygen treated animals. No change was found in nerve conduction. <strong>Conclusions:</strong> This study showed that hyperbaric oxygen therapy is a potentially effective treatment for diabetic retinopathy, improving both latency and amplitude of light evoked potentials.
文摘Background: The aim of this study was to evaluate the feasibility of a dualbolus protocol, where a first bolus of an intravascular tracer is used to measure perfusion, followed by a second bolus of a freely filtered gadolinium-containing agent to measure filtration capacity. Methods: The study was conducted in mice subjected to complete unilateral ureteral obstruction (UUO), and sham operated mice were used as controls. Dynamic contrast- en-hanced MRI was performed 2 days after surgery. Results and discussions: Mean signal-time curves of the renal cortex, renal medulla and abdominal aorta were used to calculate the relative renal blood flow (rRBF), relative renal blood volume (rRBV), mean transit time (MTT) and the glomerular transfer rate Ktrans. We demonstrated that kidneys suffering from two days of UUO showed a decrease in cortical as well as medullary rRBF compared to kidneys from sham-operated mice. Further, we found no changes in rRBV and MTT among groups, neither in the cortex nor in the medulla. The renal functional parameter Ktrans showed a tendency (but statistically insignificant) to be reduced in the ob-structed kidney compared to the sham-operated mice. Conclusions: We showed our first experiences with the consecutive use of intra- and extra-vascularly distributed agents in a renal-diseased mouse model, allowing analysis of both functional haemo- dyamics and filtration capacity in kidneys.
