In vivo analysis of intestinal permeability following hemorrhagic shock

AIM: To determine the time course of intestinal permeability changes to proteolytically-derived bowel peptides in experimental hemorrhagic shock. METHODS: We injected fluorescently-conjugated casein protein into the small bowel of anesthetized Wistar rats prior to induction of experimental hemorrhagic shock. These molecules, which fluoresce when proteolytically cleaved, were used as markers for the ability of proteolytically cleaved intestinal products to access the central circulation. Blood was serially sampled to quantify the relative change in concentration of proteolytically-cleaved particles in the systemic circulation. To provide spatial resolution of their location, particles in the mesenteric microvasculature were imaged using in vivo intravital fluorescent microscopy. The experiments were then repeated using an alternate measurement technique, fluorescein isothiocyanate(FITC)-labeled dextrans 20, to semi-quantitatively verify the ability of bowel-derived low-molecular weight molecules(< 20 k D) to access the central circulation.RESULTS: Results demonstrate a significant increase in systemic permeability to gut-derived peptides within 20 min after induction of hemorrhage(1.11 ± 0.19 vs 0.86 ± 0.07, P < 0.05) compared to control animals. Reperfusion resulted in a second, sustained increase in systemic permeability to gut-derived peptides in hemorrhaged animals compared to controls(1.2 ± 0.18 vs 0.97 ± 0.1, P < 0.05). Intravital microscopy of the mesentery also showed marked accumulation of fluorescent particles in the microcirculation of hemorrhaged animals compared to controls. These results were replicated using FITC dextrans 20 [10.85 ± 6.52 vs 3.38 ± 1.11 fluorescent intensity units(× 105, P < 0.05, hemorrhagic shock vs controls)], confirming that small bowel ischemia in response to experimental hemorrhagic shock results in marked and early increases in gut membrane permeability. CONCLUSION: Increased small bowel permeability in hemorrhagic shock may allow for systemic absorption of otherwise retained proteolytically-generated peptides, with consequent hemodynamic instability and remote organ failure.

Observed Changes in Long-Term Climatic Conditions and Inner-Regional Differences in Urban Regions of the Baltic Sea Coast

This paper presents research outcomes from an investigation into climate change and urban impacts on climate development in urban regions of the Baltic Sea coast. The cities considered were Rostock and Stockholm, and their surrounding regions. The objectives were: 1) to determine whether significant changes in temperature and precipitation have occurred and, if so, to what extent;and 2) to establish whether there is a noticeable urban heat island effect in Stockholm and the medium-sized city of Rostock. Climatic trends were detected by linear regression and the Mann- Kendall test. Different precipitation trends were detected over the whole period of observation. Average annual temperatures increased significantly in both case studies, particularly from the 1970s with highest trends in winter and lowest in autumn (Rostock) and summer (Stockholm). Although changes in temperature extremes were detected for both regions, no overall long-term trend for precipitation extremes was observed. The average temperature in the city of Rostock (Stockholm) was approximately 0.3°C to 0.6°C (1.2°C) higher than in the surrounding rural areas had seasonal variations, with maxima in the warm season. The main outcomes were that significant changes in climatic conditions, particularly temperature patterns, have been occurring in the case study regions since the 1980s, and that there is a considerable urban heat island effect in both Stockholm and Rostock. This could encourage urban planners to consider specific climatic conditions and small-scale climatic influences also in relatively small coastal urban conglomerates in mid latitudes which can follow from land use changes.