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Pathway engineering strategies for improved product yield in yeast-based industrial ethanol production 被引量:1
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作者 Aafke C.A.van Aalsta Sophie C.de Valk +3 位作者 Walter M.van Gulik mickel l.a.jansen Jack T.Pronka Robert Mans 《Synthetic and Systems Biotechnology》 SCIE 2022年第1期554-566,共13页
Product yield on carbohydrate feedstocks is a key performance indicator for industrial ethanol production with the yeast Saccharomyces cerevisiae.This paper reviews pathway engineering strategies for improving ethanol... Product yield on carbohydrate feedstocks is a key performance indicator for industrial ethanol production with the yeast Saccharomyces cerevisiae.This paper reviews pathway engineering strategies for improving ethanol yield on glucose and/or sucrose in anaerobic cultures of this yeast by altering the ratio of ethanol production,yeast growth and glycerol formation.Particular attention is paid to strategies aimed at altering energy coupling of alcoholic fermentation and to strategies for altering redox-cofactor coupling in carbon and nitrogen meta-bolism that aim to reduce or eliminate the role of glycerol formation in anaerobic redox metabolism.In addition to providing an overview of scientific advances we discuss context dependency,theoretical impact and potential for industrial application of different proposed and developed strategies. 展开更多
关键词 Saccharomyces cerevisiae Synthetic biology Metabolic engineering Biofuels Redox metabolism Energy metabolism
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