Dear Editor,The expression profile demonstrates how each gene was expressed in the target tissue or cell at a given time.Cell heterogeneity is a biological concept characterized by the presence of numerous cells with ...Dear Editor,The expression profile demonstrates how each gene was expressed in the target tissue or cell at a given time.Cell heterogeneity is a biological concept characterized by the presence of numerous cells with varying functions and expression profiles within organ/tissue samples.There is also cell-to-cell signaling,which implies that the extracellular environment and spatial location influence intracellular transcription,resulting in spatial heterogeneity of gene expression within the same cell type.展开更多
Dear Editor,Using sc RNA-seq technology, researchers may distinguish between different cell types and tissue heterogeneity as well as perform transcriptome investigations at the single-cell level (Regev et al., 2017)....Dear Editor,Using sc RNA-seq technology, researchers may distinguish between different cell types and tissue heterogeneity as well as perform transcriptome investigations at the single-cell level (Regev et al., 2017). A significant amount of single-cell transcriptome data has been produced as a result of the technology’s quick development and widespread adoption.Research on other model species has also gathered a sizable quantity of transcriptome data at the single-cell level.展开更多
Diseases of grass carps often occur in juveniles but not in adults that may have established disease resistance during development.We performed both DNA bisulfite and transcriptome sequencing on liver libraries of 1-a...Diseases of grass carps often occur in juveniles but not in adults that may have established disease resistance during development.We performed both DNA bisulfite and transcriptome sequencing on liver libraries of 1-and 3-year-old grass carps.Differential DNA-methylation analysis exhibited a declined methylation level through development.Functional annotation revealed that identified differentially methylated genes(DMGs)and differentially expressed genes were enriched in immune-related pathways such as PI3K-Akt signaling pathway and its related pathways.Both differentially methylated and differentially expressed genes were clustered into the growth-and immune-related function networks.Subcellular localization analysis indicated that the DMGs localized on cell membrane were significantly enriched in calcium signaling pathway and neuroactive ligand-receptor interaction pathway,implying the importance of G protein-coupled receptors to development.These findings will broaden our understanding of the key genes and pathways that affect the immune system at different development stages and the developing protective strategies in grass carp.展开更多
基金supported by the National Key Research and Development Program of China(2021YFD1200804,2018YFD0901201)the Strategic Priority Research Program of the Chinese Academy of Sciences(XDA24010206)。
文摘Dear Editor,The expression profile demonstrates how each gene was expressed in the target tissue or cell at a given time.Cell heterogeneity is a biological concept characterized by the presence of numerous cells with varying functions and expression profiles within organ/tissue samples.There is also cell-to-cell signaling,which implies that the extracellular environment and spatial location influence intracellular transcription,resulting in spatial heterogeneity of gene expression within the same cell type.
基金supported by the National Key Research and Development Program of China (2021YFD1200804 and2018YFD0901201)the Strategic Priority Research Program of the Chinese Academy of Sciences (XDA24010206)supported by the Wuhan Branch,Supercomputing Center,Chinese Academy of Sciences,China。
文摘Dear Editor,Using sc RNA-seq technology, researchers may distinguish between different cell types and tissue heterogeneity as well as perform transcriptome investigations at the single-cell level (Regev et al., 2017). A significant amount of single-cell transcriptome data has been produced as a result of the technology’s quick development and widespread adoption.Research on other model species has also gathered a sizable quantity of transcriptome data at the single-cell level.
基金This work is supported by the National Key Research and Development Program of China(2018YFD0900601,2018YFD0900101)Doctor Fund of Shanghai Ocean University(A2-2006-00-200301)Science and Technology Development Fund of Shanghai Ocean University(A2-2006-00-200205).
文摘Diseases of grass carps often occur in juveniles but not in adults that may have established disease resistance during development.We performed both DNA bisulfite and transcriptome sequencing on liver libraries of 1-and 3-year-old grass carps.Differential DNA-methylation analysis exhibited a declined methylation level through development.Functional annotation revealed that identified differentially methylated genes(DMGs)and differentially expressed genes were enriched in immune-related pathways such as PI3K-Akt signaling pathway and its related pathways.Both differentially methylated and differentially expressed genes were clustered into the growth-and immune-related function networks.Subcellular localization analysis indicated that the DMGs localized on cell membrane were significantly enriched in calcium signaling pathway and neuroactive ligand-receptor interaction pathway,implying the importance of G protein-coupled receptors to development.These findings will broaden our understanding of the key genes and pathways that affect the immune system at different development stages and the developing protective strategies in grass carp.
