Background:Baliospermum solanifolium is a traditional medicinal plant,locally known as“dantigaacha”from Eu-phorbiaceae family,and has been used in different diseases treatments likes helminthiasis,skin diseases,diab...Background:Baliospermum solanifolium is a traditional medicinal plant,locally known as“dantigaacha”from Eu-phorbiaceae family,and has been used in different diseases treatments likes helminthiasis,skin diseases,diabetes,snake-bite,and leukoderma.Objectives:Depending on the traditional uses of B.solanifolium and the lack of scientific works,we aimed to conduct some pharmacological tests on its leaves.Methods:Active constituents of B.solanifolium were assessed by qualitative screening.DPPH(2,2-diphenyl-1-picrylhydrazyl)was used for free radical scavenging assay.Behavioral change and mortality rate were used as indicators to assess the toxicity of B.solanifolium.The antidiabetic potential was evaluated by oral glucose toler-ance test(OGTT)and alpha-glucosidase enzyme inhibitory activity.In antidiarrheal activity,castor oil-induced mice were used.Antibacterial test was carried out by disk diffusion assay and MIC determination.Furthermore,analgesic activity was done by acetic acid-induced writhing method.Results:B.solanifolium contains alkaloids,carbohydrates,glycosides,flavonoids,tannis,phenolics etc.Acute toxicity test ensured the safety of the extract.In DPPH,the extract showed antioxidant activity with an IC50(con-centration needed to scavenge 50%DPPH)value of 61.65μg/mL.Total phenolic,flavonoid and tannin contents were determined as 124 mg GAE/g,440 mg QE/g and 344 mg GAE/g dried extract,respectively.In OGTT,the ex-tract reduced the blood glucose level significantly.In the alpha-glucosidase inhibitory activity,the plant showed a concentration-dependent reduction of the enzyme at IC50 of 8.53 mg/mL.In the antidiarrheal test,B.solanifolium inhibit defecation 30.69%and 53.41%at dose 250 mg/kg and 500 mg/kg,respectively.The extract also showed antibacterial activity against four bacterial strains in the disk diffusion method and minimum inhibitory concen-tration(MIC)values were 62.5μg/mL,62.5μg/mL,125μg/mL and 62.5μg/mL against four bacterial strains,independently.In analgesic activity test,the extract reduced the writhing impulse up to 48.51%at 500 mg/kg dose.Conclusion:B.solanifolium contains many active phytoconstituents which may be the possible candidates for targeting diabetes,microbial infection,diarrhea and pain in the future.展开更多
文摘Background:Baliospermum solanifolium is a traditional medicinal plant,locally known as“dantigaacha”from Eu-phorbiaceae family,and has been used in different diseases treatments likes helminthiasis,skin diseases,diabetes,snake-bite,and leukoderma.Objectives:Depending on the traditional uses of B.solanifolium and the lack of scientific works,we aimed to conduct some pharmacological tests on its leaves.Methods:Active constituents of B.solanifolium were assessed by qualitative screening.DPPH(2,2-diphenyl-1-picrylhydrazyl)was used for free radical scavenging assay.Behavioral change and mortality rate were used as indicators to assess the toxicity of B.solanifolium.The antidiabetic potential was evaluated by oral glucose toler-ance test(OGTT)and alpha-glucosidase enzyme inhibitory activity.In antidiarrheal activity,castor oil-induced mice were used.Antibacterial test was carried out by disk diffusion assay and MIC determination.Furthermore,analgesic activity was done by acetic acid-induced writhing method.Results:B.solanifolium contains alkaloids,carbohydrates,glycosides,flavonoids,tannis,phenolics etc.Acute toxicity test ensured the safety of the extract.In DPPH,the extract showed antioxidant activity with an IC50(con-centration needed to scavenge 50%DPPH)value of 61.65μg/mL.Total phenolic,flavonoid and tannin contents were determined as 124 mg GAE/g,440 mg QE/g and 344 mg GAE/g dried extract,respectively.In OGTT,the ex-tract reduced the blood glucose level significantly.In the alpha-glucosidase inhibitory activity,the plant showed a concentration-dependent reduction of the enzyme at IC50 of 8.53 mg/mL.In the antidiarrheal test,B.solanifolium inhibit defecation 30.69%and 53.41%at dose 250 mg/kg and 500 mg/kg,respectively.The extract also showed antibacterial activity against four bacterial strains in the disk diffusion method and minimum inhibitory concen-tration(MIC)values were 62.5μg/mL,62.5μg/mL,125μg/mL and 62.5μg/mL against four bacterial strains,independently.In analgesic activity test,the extract reduced the writhing impulse up to 48.51%at 500 mg/kg dose.Conclusion:B.solanifolium contains many active phytoconstituents which may be the possible candidates for targeting diabetes,microbial infection,diarrhea and pain in the future.
