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An Experimental Study on the Expression of Heme Oxygenase-2 mRNA in Hirschsprung's Disease
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作者 ZHU min(朱珉) +3 位作者 WEI mingfa(魏明发) LIU Fang(刘芳) 《Journal of Huazhong University of Science and Technology(Medical Sciences)》 SCIE CAS 2002年第2期152-154,共3页
Summary: In order to investigate the relationship between the expression of heme oxygenase-2 (HO-2) mRNA and the pathogenesis of Hirschsprung's disease (HD), total ribonucleic acid (RNA) was extracted in the agang... Summary: In order to investigate the relationship between the expression of heme oxygenase-2 (HO-2) mRNA and the pathogenesis of Hirschsprung's disease (HD), total ribonucleic acid (RNA) was extracted in the aganglionic and ganglionic segments of colon respectively from 15 cases of HD. The single-stranded cDNA of HO-2 was synthesized and further amplified by reverse transcription-polymerase chain reaction (RT-PCR). The expression of HO-2 mRNA was normal in ganglionic segments, but absent in aganglionic segments. It is concluded that the absence of HO-2 mRNA expression may be an important mechanism responsible for HD. 展开更多
关键词 Hirschsprung's disease heme oxygenase-2 gene expression reverse transcription-polymerase chain reaction
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